|
| Status |
Public on Aug 29, 2012 |
| Title |
IDE2+FGF2a |
| Sample type |
RNA |
| |
|
| Source name |
Embryonic Stem Cells
|
| Organism |
Mus musculus |
| Characteristics |
cell line: E14TG2a
differentiation medium: 1uM IDE2 and then 500ng/ml of FGF2
|
| Treatment protocol |
Cells were cultured in differentiation medium containing 1uM IDE2 for the first 6 days, IDE2+FGF2 and IDE2+FGF10 conditions were then switched to differentiation medium containing either 500ng/ml of FGF2 or 25ng/ml of FGF10 for 5 days. IDE2 only condition continued differentiation in medim containing 1uM for all 11 days. Differentiation medium was comprised of Iscoves Medium and Ham F12 medium with additional supplements including N2, B27, 1% FCS, 1X PenStrep, 2uM L-Glutamine, 1-Thioglycerol and Ascorbic Acid.
|
| Growth protocol |
Cells were maintained in GMEM medium supplemented with 1X PenStrep, 1X Pyruvate, 10% FCS, B-Mercaptoethanol, 2uM L-Glutamine, and CHO-LIF Conditioned Medium on 35mm Plates until differentiation began
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by DNase I treatment with Ambion Turbo DNASE. Treated RNA was assessed and quantified with nanodrop spectrophotometer.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
Replicate 1
|
| Data processing |
The data were normalised using quantile normalisation with Illumina BeadStudio
|
| |
|
| Submission date |
Aug 28, 2012 |
| Last update date |
Aug 29, 2012 |
| Contact name |
Todd James Jensen |
| E-mail(s) |
tjensen@uchc.edu
|
| Organization name |
University of Connecticut Health Center
|
| Department |
Center for Vascular Biology
|
| Lab |
Finck Lab
|
| Street address |
263 Farmington Avenue MC3501
|
| City |
Farmington |
| State/province |
CT |
| ZIP/Postal code |
06030 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE40426 |
A modified lung allograft renal capsule model to investigate the differentiation of lung epithelial cells from murine embryonic stem cells |
|
