|
| Status |
Public on Nov 01, 2012 |
| Title |
CD14+CD16- monocytes donor2 |
| Sample type |
RNA |
| |
|
| Source name |
Healthy donor2
|
| Organism |
Homo sapiens |
| Characteristics |
cell: primary cells
cell population: CD14+CD16- monocytes
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using Rneasy Microquit (Qiagen).
|
| Label |
biotin
|
| Label protocol |
100 ng of polysomal-bound RNA were employed to synthesize double-stranded cDNA using 2 successive reverse-transcription reactions according to the std affymetrix protocol
|
| |
|
| Hybridization protocol |
Labelled DNA was hybrized on the Affymetrix Human Gene 1.1 ST processed on an Affymetrix GeneTitan device.
|
| Scan protocol |
Array scanning was performed according to the manufacturer's instruction (Affymetrix)
|
| Description |
RMA expression value derived from Genesprings GX 7.3 (Agilent)
|
| Data processing |
Statistical analysis were performed using GenSpring GX 7.3 (Agilent). Expression data was normalized using the RMA algorithm. Probes with an expression lower than 25 Units were filtered out.
|
| |
|
| Submission date |
Aug 30, 2012 |
| Last update date |
Nov 01, 2012 |
| Contact name |
Maxime Touzot |
| E-mail(s) |
mtouzot@hotmail.com
|
| Organization name |
Institut Curie
|
| Lab |
U932
|
| Street address |
26 rue d'ULM
|
| City |
PAris |
| ZIP/Postal code |
75005 |
| Country |
France |
| |
|
| Platform ID |
GPL11532 |
| Series (1) |
| GSE40484 |
Human inflammatory dendritic cells induce Th17 differentiation |
|
