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Sample GSM994844 Query DataSets for GSM994844
Status Public on Nov 01, 2012
Title CD14dimCD16+ monocytes donor2
Sample type RNA
 
Source name Healthy donor2
Organism Homo sapiens
Characteristics cell: primary cells
cell population: CD14dimCD16+ monocytes
Extracted molecule total RNA
Extraction protocol RNA was extracted using Rneasy Microquit (Qiagen).
Label biotin
Label protocol 100 ng of polysomal-bound RNA were employed to synthesize double-stranded cDNA using 2 successive reverse-transcription reactions according to the std affymetrix protocol
 
Hybridization protocol Labelled DNA was hybrized on the Affymetrix Human Gene 1.1 ST processed on an Affymetrix GeneTitan device.
Scan protocol Array scanning was performed according to the manufacturer's instruction (Affymetrix)
Description RMA expression value derived from Genesprings GX 7.3 (Agilent)
Data processing Statistical analysis were performed using GenSpring GX 7.3 (Agilent). Expression data was normalized using the RMA algorithm. Probes with an expression lower than 25 Units were filtered out.
 
Submission date Aug 30, 2012
Last update date Nov 01, 2012
Contact name Maxime Touzot
E-mail(s) mtouzot@hotmail.com
Organization name Institut Curie
Lab U932
Street address 26 rue d'ULM
City PAris
ZIP/Postal code 75005
Country France
 
Platform ID GPL11532
Series (1)
GSE40484 Human inflammatory dendritic cells induce Th17 differentiation

Data table header descriptions
ID_REF
VALUE log(2) RMA

Data table
ID_REF VALUE
7892501 4.986732
7892502 7.560878
7892503 4.594487
7892504 8.249457
7892505 3.437309
7892506 7.973574
7892507 4.261641
7892508 8.325884
7892509 10.16999
7892510 6.881866
7892511 6.734894
7892512 5.37173
7892513 4.399185
7892514 10.08766
7892515 8.525298
7892516 3.510496
7892517 6.579247
7892518 3.704725
7892519 6.526527
7892520 8.850888

Total number of rows: 33297

Table truncated, full table size 549 Kbytes.




Supplementary file Size Download File type/resource
GSM994844_IS09_ARN0005_s1h1_E05_hGene.CEL.gz 4.5 Mb (ftp)(http) CEL
Processed data included within Sample table

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