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Sample GSM994845 Query DataSets for GSM994845
Status Public on Nov 01, 2012
Title Dendritic cells BDCA1 donor2
Sample type RNA
 
Source name Healthy donor2
Organism Homo sapiens
Characteristics cell: primary cells
cell population: Dendritic cells BDCA1
Extracted molecule total RNA
Extraction protocol RNA was extracted using Rneasy Microquit (Qiagen).
Label biotin
Label protocol 100 ng of polysomal-bound RNA were employed to synthesize double-stranded cDNA using 2 successive reverse-transcription reactions according to the std affymetrix protocol
 
Hybridization protocol Labelled DNA was hybrized on the Affymetrix Human Gene 1.1 ST processed on an Affymetrix GeneTitan device.
Scan protocol Array scanning was performed according to the manufacturer's instruction (Affymetrix)
Description RMA expression value derived from Genesprings GX 7.3 (Agilent)
Data processing Statistical analysis were performed using GenSpring GX 7.3 (Agilent). Expression data was normalized using the RMA algorithm. Probes with an expression lower than 25 Units were filtered out.
 
Submission date Aug 30, 2012
Last update date Nov 01, 2012
Contact name Maxime Touzot
E-mail(s) mtouzot@hotmail.com
Organization name Institut Curie
Lab U932
Street address 26 rue d'ULM
City PAris
ZIP/Postal code 75005
Country France
 
Platform ID GPL11532
Series (1)
GSE40484 Human inflammatory dendritic cells induce Th17 differentiation

Data table header descriptions
ID_REF
VALUE log(2) RMA

Data table
ID_REF VALUE
7892501 5.81431
7892502 7.96018
7892503 3.068514
7892504 8.431504
7892505 4.355692
7892506 7.84167
7892507 3.414527
7892508 8.627218
7892509 10.58099
7892510 6.569221
7892511 6.389482
7892512 4.746629
7892513 5.885626
7892514 10.76501
7892515 8.870969
7892516 3.074444
7892517 7.085774
7892518 3.63126
7892519 6.862917
7892520 8.624969

Total number of rows: 33297

Table truncated, full table size 549 Kbytes.




Supplementary file Size Download File type/resource
GSM994845_IS09_ARN0006_s1h1_F05_hGene.CEL.gz 4.6 Mb (ftp)(http) CEL
Processed data included within Sample table

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