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Sample GSM994854 Query DataSets for GSM994854
Status Public on Nov 01, 2012
Title Inflammatory Dendritic cells donor5
Sample type RNA
 
Source name Ovarian cancer donor 5
Organism Homo sapiens
Characteristics cell: cells isolated from ascites
cell population: inflammatory Dendritic cells
Extracted molecule total RNA
Extraction protocol RNA was extracted using Rneasy Microquit (Qiagen).
Label biotin
Label protocol 100 ng of polysomal-bound RNA were employed to synthesize double-stranded cDNA using 2 successive reverse-transcription reactions according to the std affymetrix protocol
 
Hybridization protocol Labelled DNA was hybrized on the Affymetrix Human Gene 1.1 ST processed on an Affymetrix GeneTitan device.
Scan protocol Array scanning was performed according to the manufacturer's instruction (Affymetrix)
Description RMA expression value derived from Genesprings GX 7.3 (Agilent)
Data processing Statistical analysis were performed using GenSpring GX 7.3 (Agilent). Expression data was normalized using the RMA algorithm. Probes with an expression lower than 25 Units were filtered out.
 
Submission date Aug 30, 2012
Last update date Nov 01, 2012
Contact name Maxime Touzot
E-mail(s) mtouzot@hotmail.com
Organization name Institut Curie
Lab U932
Street address 26 rue d'ULM
City PAris
ZIP/Postal code 75005
Country France
 
Platform ID GPL11532
Series (1)
GSE40484 Human inflammatory dendritic cells induce Th17 differentiation

Data table header descriptions
ID_REF
VALUE log(2) RMA

Data table
ID_REF VALUE
7892501 4.407354
7892502 4.379241
7892503 3.6988
7892504 9.405
7892505 3.792734
7892506 5.426863
7892507 4.291878
7892508 5.51729
7892509 10.87233
7892510 3.445292
7892511 4.201911
7892512 3.345087
7892513 2.609974
7892514 10.31059
7892515 9.854038
7892516 2.629086
7892517 4.783405
7892518 3.165712
7892519 3.748755
7892520 9.075474

Total number of rows: 33297

Table truncated, full table size 549 Kbytes.




Supplementary file Size Download File type/resource
GSM994854_IS09_ARN0015_s1h1_G07_hGene.CEL.gz 3.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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