|
| Status |
Public on Jun 20, 2013 |
| Title |
PDGF-BB, rep2 |
| Sample type |
RNA |
| |
|
| Source name |
exosomes derived from cardiomyocytes, PDGF-BB treated
|
| Organism |
Mus musculus |
| Characteristics |
cell type: exosomes derived from HL-1 cardiomyocytes
treatment: PDGF-BB
|
| Treatment protocol |
HL-1 cells were treated with or without growth factor (10ng/mL TGF-beta2 or 100ng/mL PDGF-BB, Invitrogen), and incubated in serum-free media for 23h before collection of media.
|
| Growth protocol |
HL-1 cells were grown in T75 flasks and kept according to protocol (serum was ultracentrifuged before use).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Whole exosome RNA was purified with the RNeasy Fibrous Tissue Kit (Qiagen).
|
| Label |
Cy3
|
| Label protocol |
Illumina TotalPrep RNA Amplification Kit was used.
|
| |
|
| Hybridization protocol |
Performed according to Illumina protocol.
|
| Scan protocol |
Illumina BeadStation 500GX according to Illumina protocol.
|
| Description |
5773660151_E
Exosomal RNA.
|
| Data processing |
Cubic spline normalization, subtracted background. Illumina GenomeStudio was used.
|
| |
|
| Submission date |
Aug 30, 2012 |
| Last update date |
Jun 20, 2013 |
| Contact name |
Nina Gennebäck |
| E-mail(s) |
nina.genneback@ncl.ac.uk
|
| Organization name |
Umeå University
|
| Department |
Public health and clinical medicin/medicin
|
| Street address |
NUS 6M
|
| City |
Umeå |
| ZIP/Postal code |
901 86 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE40503 |
Study of transcriptional content in exosomes derived from untreated and growth factor-treated cultured cardiomyocytes (HL-1) |
|
