|
Status |
Public on Oct 17, 2012 |
Title |
Xbp1 ko, IRE1 siRNA, biological rep3 |
Sample type |
RNA |
|
|
Source name |
Xbp1 ko, IRE1 siRNA injected, 8 days
|
Organism |
Mus musculus |
Characteristics |
tissue: Liver gender: male stain: c57BL/6 genotype: Xbp1 ko age: 9 weeks
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol (Invitrogen) extraction of total RNA followed by cleanup using RNeasy MinElute Cleanup Kit (Qiagen) was performed according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1.2 ug of total RNA.
|
|
|
Hybridization protocol |
Affymetrix Standard Protocol
|
Scan protocol |
AGCC GeneTitan Wash and Scan
|
Data processing |
We use the R bioconductor suite to normalize the Affymetrix data. The package we use for RMA is in the affy package and the version we currently use is 1.20.2 .
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|
|
Submission date |
Aug 31, 2012 |
Last update date |
Oct 17, 2012 |
Contact name |
Ann-Hwee Lee |
E-mail(s) |
ahlee@hsph.harvard.edu
|
Organization name |
Harvard School of public health
|
Street address |
651 Huntington Ave, FXB/205
|
City |
Boston |
State/province |
MA |
ZIP/Postal code |
02115 |
Country |
USA |
|
|
Platform ID |
GPL11180 |
Series (1) |
GSE40515 |
Expression data from IRE1a or XBP1 deficient mouse liver |
|