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Sample GSM995464 Query DataSets for GSM995464
Status Public on Oct 17, 2012
Title Xbp1 ko, IRE1 siRNA, biological rep3
Sample type RNA
 
Source name Xbp1 ko, IRE1 siRNA injected, 8 days
Organism Mus musculus
Characteristics tissue: Liver
gender: male
stain: c57BL/6
genotype: Xbp1 ko
age: 9 weeks
Extracted molecule total RNA
Extraction protocol Trizol (Invitrogen) extraction of total RNA followed by cleanup using RNeasy MinElute Cleanup Kit (Qiagen) was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1.2 ug of total RNA.
 
Hybridization protocol Affymetrix Standard Protocol
Scan protocol AGCC GeneTitan Wash and Scan
Data processing We use the R bioconductor suite to normalize the Affymetrix data. The package we use for RMA is in the affy package and the version we currently use is 1.20.2 .
 
Submission date Aug 31, 2012
Last update date Oct 17, 2012
Contact name Ann-Hwee Lee
E-mail(s) ahlee@hsph.harvard.edu
Organization name Harvard School of public health
Street address 651 Huntington Ave, FXB/205
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL11180
Series (1)
GSE40515 Expression data from IRE1a or XBP1 deficient mouse liver

Data table header descriptions
ID_REF
VALUE RMA Signal Intensity

Data table
ID_REF VALUE
1415670_PM_at 68
1415671_PM_at 213
1415672_PM_at 296
1415673_PM_at 20
1415674_PM_a_at 72
1415675_PM_at 39
1415676_PM_a_at 379
1415677_PM_at 282
1415678_PM_at 262
1415679_PM_at 491
1415680_PM_at 45
1415681_PM_at 228
1415682_PM_at 40
1415683_PM_at 382
1415684_PM_at 70
1415685_PM_at 71
1415686_PM_at 177
1415687_PM_a_at 267
1415688_PM_at 285
1415689_PM_s_at 42

Total number of rows: 45037

Table truncated, full table size 737 Kbytes.




Supplementary file Size Download File type/resource
GSM995464_Xbp1_ko_siLuc3.CEL.gz 1.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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