|
| Status |
Public on Sep 01, 2013 |
| Title |
lqz014 tumor |
| Sample type |
RNA |
| |
|
| Source name |
HEPATOCELLULAR CARCINOMA
|
| Organism |
Mus musculus |
| Characteristics |
tissue: HEPATOCELLULAR CARCINOMA
prom1 status: tumor N/A
strain: C57BL/6
disease state: tumor
|
| Growth protocol |
A small chuck of normal or tumor tissue was collected and frozen on dry ice immediately after animal was euthanized. For Prom1+ and Prom1- cells isolation, tissues were dissociated into single cells and sorted using anti-Prom1 MicroBeads (Miltenyi Biotec).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total mRNA was extracted from frozen tissues or sorted Prom1+ and Prom1- cells using PicoPureTM extraction kit (Arcturus).
|
| Label |
Biotin
|
| Label protocol |
standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
standard Affymetrix protocol
|
| Scan protocol |
standard Affymetrix protocol
|
| Description |
tumor
|
| Data processing |
The data were analyzed with RMA as implemented in Partek Genomics Suite 6.6
|
| |
|
| Submission date |
Sep 05, 2012 |
| Last update date |
Sep 01, 2013 |
| Contact name |
David Finkelstein |
| E-mail(s) |
david.finkelstein@stjude.org
|
| Phone |
9014953931
|
| Organization name |
St Jude Children's Research Hospital
|
| Department |
Computational Biology
|
| Street address |
332 N. Lauderdale St.
|
| City |
Memphis |
| State/province |
TN |
| ZIP/Postal code |
38105 |
| Country |
USA |
| |
|
| Platform ID |
GPL11180 |
| Series (1) |
| GSE40634 |
Cancers originate preferentially in adult tissue stem cells |
|
