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Sample GSM998447 Query DataSets for GSM998447
Status Public on Sep 01, 2013
Title lqz031 tumor
Sample type RNA
 
Source name SQUAMOUS CELL CARCINOMA OF SKIN
Organism Mus musculus
Characteristics tissue: SQUAMOUS CELL CARCINOMA OF SKIN
prom1 status: tumor N/A
strain: C57BL/6
disease state: tumor
Growth protocol A small chuck of normal or tumor tissue was collected and frozen on dry ice immediately after animal was euthanized. For Prom1+ and Prom1- cells isolation, tissues were dissociated into single cells and sorted using anti-Prom1 MicroBeads (Miltenyi Biotec).
Extracted molecule total RNA
Extraction protocol Total mRNA was extracted from frozen tissues or sorted Prom1+ and Prom1- cells using PicoPureTM extraction kit (Arcturus).
Label Biotin
Label protocol standard Affymetrix protocol
 
Hybridization protocol standard Affymetrix protocol
Scan protocol standard Affymetrix protocol
Description tumor
Data processing The data were analyzed with RMA as implemented in Partek Genomics Suite 6.6
 
Submission date Sep 05, 2012
Last update date Sep 01, 2013
Contact name David Finkelstein
E-mail(s) david.finkelstein@stjude.org
Phone 9014953931
Organization name St Jude Children's Research Hospital
Department Computational Biology
Street address 332 N. Lauderdale St.
City Memphis
State/province TN
ZIP/Postal code 38105
Country USA
 
Platform ID GPL11180
Series (1)
GSE40634 Cancers originate preferentially in adult tissue stem cells

Data table header descriptions
ID_REF
VALUE RMA defined log2 signal

Data table
ID_REF VALUE
1415670_PM_at 9.19506
1415671_PM_at 9.5313
1415672_PM_at 10.2795
1415673_PM_at 8.37128
1415674_PM_a_at 8.70018
1415675_PM_at 7.98399
1415676_PM_a_at 10.0638
1415677_PM_at 8.8738
1415678_PM_at 9.31463
1415679_PM_at 10.6865
1415680_PM_at 8.30628
1415681_PM_at 8.97238
1415682_PM_at 7.22918
1415683_PM_at 10.2715
1415684_PM_at 7.8092
1415685_PM_at 7.32576
1415686_PM_at 8.45055
1415687_PM_a_at 9.51716
1415688_PM_at 9.78061
1415689_PM_s_at 7.96073

Total number of rows: 45141

Table truncated, full table size 983 Kbytes.




Supplementary file Size Download File type/resource
GSM998447_lqz031-htmg430pm16.CEL.gz 2.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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