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    Oplah 5-oxoprolinase (ATP-hydrolysing) [ Mus musculus (house mouse) ]

    Gene ID: 75475, updated on 12-May-2024

    Summary

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    Official Symbol
    Oplahprovided by MGI
    Official Full Name
    5-oxoprolinase (ATP-hydrolysing)provided by MGI
    Primary source
    MGI:MGI:1922725
    See related
    Ensembl:ENSMUSG00000022562 AllianceGenome:MGI:1922725
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Mus musculus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
    Also known as
    5-OPase; 1700010G02Rik
    Summary
    Predicted to enable 5-oxoprolinase (ATP-hydrolyzing) activity and identical protein binding activity. Predicted to be involved in glutathione metabolic process. Is expressed in several structures, including alimentary system; cranium; genitourinary system; lung; and thymus. Orthologous to human OPLAH (5-oxoprolinase, ATP-hydrolysing). [provided by Alliance of Genome Resources, Apr 2022]
    Expression
    Broad expression in kidney adult (RPKM 41.9), adrenal adult (RPKM 38.5) and 24 other tissues See more
    Orthologs
    human all
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    Genomic context

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    See Oplah in Genome Data Viewer
    Location:
    15 D3; 15 35.67 cM
    Exon count:
    30
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCm39 (GCF_000001635.27) 15 NC_000081.7 (76180803..76215381, complement)
    108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 15 NC_000081.6 (76296603..76331181, complement)

    Chromosome 15 - NC_000081.7Genomic Context describing neighboring genes Neighboring gene spermatogenesis and centriole associated 1 Neighboring gene myocardial infraction associated transcript 2 Neighboring gene microRNA 3079 Neighboring gene sphingomyelin phosphodiesterase 5 Neighboring gene STARR-seq mESC enhancer starr_39050 Neighboring gene STARR-positive B cell enhancer ABC_E7384 Neighboring gene exosome component 4 Neighboring gene GPI anchor attachment protein 1 Neighboring gene ribosomal protein S6, pseudogene 1

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: Mouse ENCODE transcriptome data
    • Description: RNA profiling data sets generated by the Mouse ENCODE project.
    • BioProject: PRJNA66167
    • Publication: PMID 25409824
    • Analysis date: n/a

    Bibliography

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    Related articles in PubMed

    1. OPLAH ablation leads to accumulation of 5-oxoproline, oxidative stress, fibrosis, and elevated fillings pressures: a murine model for heart failure with a preserved ejection fraction. van der Pol A, et al. Cardiovasc Res, 2018 Dec 1. PMID 30032247
    2. Accumulation of 5-oxoproline in myocardial dysfunction and the protective effects of OPLAH. van der Pol A, et al. Sci Transl Med, 2017 Nov 8. PMID 29118264
    3. Metabolomic analysis via reversed-phase ion-pairing liquid chromatography coupled to a stand alone orbitrap mass spectrometer. Lu W, et al. Anal Chem, 2010 Apr 15. PMID 20349993, Free PMC Article
    4. A futile cycle, formed between two ATP-dependant gamma-glutamyl cycle enzymes, gamma-glutamyl cysteine synthetase and 5-oxoprolinase: the cause of cellular ATP depletion in nephrotic cystinosis? Kumar A, et al. J Biosci, 2010 Mar. PMID 20413906
    5. Lsd1 Ablation Triggers Metabolic Reprogramming of Brown Adipose Tissue. Duteil D, et al. Cell Rep, 2016 Oct 18. PMID 27760309, Free PMC Article

    See all (21) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Oplah full body knock-out mice had higher 5-oxoproline levels coupled to increased oxidative stress, myocardial fibrosis and elevated filling pressures reminiscent of heart failure with preserved ejection fraction.
      Title: OPLAH ablation leads to accumulation of 5-oxoproline, oxidative stress, fibrosis, and elevated fillings pressures: a murine model for heart failure with a preserved ejection fraction.
    2. OPLAH depletion occurred as a result of cardiac injury, leading to elevated 5-oxoproline and oxidative stress, whereas OPLAH overexpression improved cardiac function after ischemic injury.
      Title: Accumulation of 5-oxoproline in myocardial dysfunction and the protective effects of OPLAH.
    3. the cause of cellular ATP depletion in nephrotic cystinosis may be the futile cycle, formed between two ATP-dependant gamma-glutamyl cycle enzymes, gamma-glutamyl cysteine synthetase and 5-oxoprolinase
      Title: A futile cycle, formed between two ATP-dependant gamma-glutamyl cycle enzymes, gamma-glutamyl cysteine synthetase and 5-oxoprolinase: the cause of cellular ATP depletion in nephrotic cystinosis?
    Submit: New GeneRIF Correction

    Variation

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    Alleles

    Alleles of this type are documented at Mouse Genome Informatics  (MGI)
    • Endonuclease-mediated (2) 
    • Targeted (2) 

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Clone Names

    • MGC28663, MGC36824

    Gene Ontology Provided by MGI

    Function Evidence Code Pubs
    enables 5-oxoprolinase (ATP-hydrolyzing) activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables 5-oxoprolinase (ATP-hydrolyzing) activity ISS
    Inferred from Sequence or Structural Similarity
    more info
    		 PubMed 
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables catalytic activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables hydrolase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables identical protein binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables nucleotide binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in glutathione metabolic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within glutathione metabolic process ISS
    Inferred from Sequence or Structural Similarity
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    is_active_in cytosol IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  

    General protein information

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    Preferred Names
    5-oxoprolinase
    Names
    5-oxo-L-prolinase
    pyroglutamase
    NP_694762.1
    XP_006521570.1
    XP_006521571.1
    XP_006521572.1
    XP_030104666.1
    XP_030104667.1

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_153122.3 → NP_694762.1  5-oxoprolinase

      See identical proteins and their annotated locations for NP_694762.1

      Status: VALIDATED

      Source sequence(s)
      AC155074
      Consensus CDS
      CCDS27565.1
      UniProtKB/Swiss-Prot
      Q8K010, Q8R3K2
      Related
      ENSMUSP00000023222.7, ENSMUST00000023222.13
      Conserved Domains (1) summary
      PLN02666
      Location:1 → 1258
      PLN02666; 5-oxoprolinase

    RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCm39 C57BL/6J

    Genomic

    1. NC_000081.7 Reference GRCm39 C57BL/6J

      Range
      76180803..76215381 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_006521509.3 → XP_006521572.1  5-oxoprolinase isoform X3

      See identical proteins and their annotated locations for XP_006521572.1

      UniProtKB/Swiss-Prot
      Q8K010, Q8R3K2
      Conserved Domains (1) summary
      PLN02666
      Location:1 → 1258
      PLN02666; 5-oxoprolinase

    2. XM_030248806.2 → XP_030104666.1  5-oxoprolinase isoform X3

      UniProtKB/Swiss-Prot
      Q8K010, Q8R3K2
      Conserved Domains (1) summary
      PLN02666
      Location:1 → 1258
      PLN02666; 5-oxoprolinase

    3. XM_006521508.5 → XP_006521571.1  5-oxoprolinase isoform X2

      Conserved Domains (1) summary
      PLN02666
      Location:41 → 1298
      PLN02666; 5-oxoprolinase

    4. XM_006521507.5 → XP_006521570.1  5-oxoprolinase isoform X1

      Conserved Domains (2) summary
      PLN02666
      Location:97 → 1354
      PLN02666; 5-oxoprolinase
      pfam15058
      Location:1 → 138
      Speriolin_N; Speriolin N-terminus

    5. XM_030248807.2 → XP_030104667.1  5-oxoprolinase isoform X4

      Conserved Domains (1) summary
      PLN02666
      Location:17 → 1134
      PLN02666; 5-oxoprolinase

    RNA

    1. XR_384054.5 RNA Sequence

    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q8K010.1 GenPept UniProtKB/Swiss-Prot:Q8K010

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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