Dagla diacylglycerol lipase, alpha [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Daglaprovided by MGI
Official Full Name: diacylglycerol lipase, alphaprovided by MGI
Primary source: MGI:MGI:2677061
See related: Ensembl:ENSMUSG00000035735 AllianceGenome:MGI:2677061
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Nsddr
Summary: Enables hydrolase activity. Involved in G protein-coupled glutamate receptor signaling pathway; regulation of neuroinflammatory response; and retrograde trans-synaptic signaling by endocannabinoid. Acts upstream of or within several processes, including arachidonic acid metabolic process; endocannabinoid signaling pathway; and neurotransmitter biosynthetic process. Located in dendritic spine membrane; postsynaptic membrane; and varicosity. Is integral component of postsynaptic membrane. Is expressed in brain and olfactory epithelium. Orthologous to human DAGLA (diacylglycerol lipase alpha). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Broad expression in cortex adult (RPKM 16.8), cerebellum adult (RPKM 13.6) and 22 other tissues See more
Orthologs: human all
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Genomic context

Location:: 19 A; 19 6.55 cM

Exon count:: 22

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	19	NC_000085.7 (10222629..10282323, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	19	NC_000085.6 (10245265..10304877, complement)

Chromosome 19 - NC_000085.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Astrocytic Dagla Deletion Decreases Hedonic Feeding in Female Mice.
Title: Astrocytic Dagla Deletion Decreases Hedonic Feeding in Female Mice.
Tanycyte specific ablation of diacylglycerol lipase alpha stimulates the hypothalamic-pituitary-thyroid axis by decreasing the endocannabinoid mediated inhibition of TRH release.
Title: Tanycyte specific ablation of diacylglycerol lipase alpha stimulates the hypothalamic-pituitary-thyroid axis by decreasing the endocannabinoid mediated inhibition of TRH release.
Results demonstrate that mice with a deletion of the DAGL-alpha enzyme show a phenotype of increased emotional or stress-related behaviors. This is characterized by a reduced exploration of the central area of the open field, a maternal neglect behavior, a fear extinction deficit, increased behavioral despair, increased anxiety-related behaviors in the light/dark box, and reduced hippocampal neurogenesis
Title: Anxiety, Stress, and Fear Response in Mice With Reduced Endocannabinoid Levels.
Authors demonstrated that phospholipase D and diacylglycerol lipase were required for providing arachidonic acid for prostaglandin E(2) biosynthesis.
Title: Francisella tularensis LVS induction of prostaglandin biosynthesis by infected macrophages requires specific host phospholipases and lipid phosphatases.
In corpus striatum diacylglycerol lipase-alpha activity is regulated by calcium/calmodulin dependent protein kinase (CaMK-II).
Title: CaMKII regulates diacylglycerol lipase-α and striatal endocannabinoid signaling.
Endocannabinoid 2-arachidonoylglycerol produced by DGLalpha, but not by DGLbeta, mediates retrograde suppression at central synapses.
Title: The endocannabinoid 2-arachidonoylglycerol produced by diacylglycerol lipase alpha mediates retrograde suppression of synaptic transmission.
DAGLalpha is the major biosynthetic enzyme for 2-AG in the nervous system and reveal an essential role for this enzyme in regulating retrograde synaptic plasticity and adult neurogenesis.
Title: Loss of retrograde endocannabinoid signaling and reduced adult neurogenesis in diacylglycerol lipase knock-out mice.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (2)
Gene trapped (1)
Targeted (8) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables acylglycerol lipase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables hydrolase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables lipoprotein lipase activity	IBA Inferred from Biological aspect of Ancestor more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in G protein-coupled glutamate receptor signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in arachidonic acid metabolic process	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of arachidonic acid metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in arachidonic acid metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in arachidonic acid metabolic process	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within cannabinoid biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in diacylglycerol catabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in diacylglycerol catabolic process	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within lipid catabolic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within lipid metabolic process	IEA Inferred from Electronic Annotation more info
involved_in lipid metabolic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within monoacylglycerol biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within neuroblast proliferation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within neurogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of neuroinflammatory response	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in retrograde trans-synaptic signaling by endocannabinoid	IBA Inferred from Biological aspect of Ancestor more info
involved_in retrograde trans-synaptic signaling by endocannabinoid	IDA Inferred from Direct Assay more info	PubMed
involved_in retrograde trans-synaptic signaling by endocannabinoid	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in retrograde trans-synaptic signaling by endocannabinoid	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in cell projection	IEA Inferred from Electronic Annotation more info
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
is_active_in dendrite membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in dendrite membrane	ISO Inferred from Sequence Orthology more info
located_in dendritic spine membrane	IDA Inferred from Direct Assay more info	PubMed
located_in early endosome membrane	ISO Inferred from Sequence Orthology more info
located_in endosome	IEA Inferred from Electronic Annotation more info
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in postsynaptic density membrane	ISO Inferred from Sequence Orthology more info
is_active_in postsynaptic membrane	IBA Inferred from Biological aspect of Ancestor more info
is_active_in postsynaptic membrane	IDA Inferred from Direct Assay more info	PubMed
located_in synapse	IEA Inferred from Electronic Annotation more info
located_in varicosity	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: diacylglycerol lipase-alpha

Names: DAGL-alpha; DGL-alpha; neural stem cell-derived dendrite regulator; sn1-specific diacylglycerol lipase alpha

NP_932782.2

EC 3.1.1.116

XP_017173702.1

EC 3.1.1.116

XP_036017498.1

EC 3.1.1.116

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_198114.2 → NP_932782.2 diacylglycerol lipase-alpha

See identical proteins and their annotated locations for NP_932782.2

Status: VALIDATED

Source sequence(s)

AK147384, BC112414, BP765314, CF539357, CJ127451

Consensus CDS

CCDS29574.1

UniProtKB/Swiss-Prot

A8WFL4, Q6WQJ1, Q6ZQ76

UniProtKB/TrEMBL

A4FU75

Related

ENSMUSP00000046358.5, ENSMUST00000039327.11

Conserved Domains (1) summary

cd00519
Location:288 → 529

Lipase_3; Lipase (class 3). Lipases are esterases that can hydrolyze long-chain acyl-triglycerides into di- and monoglycerides, glycerol, and free fatty acids at a water/lipid interface. A typical feature of lipases is "interfacial activation," the process of ...

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000085.7 Reference GRCm39 C57BL/6J

Range

10222629..10282323 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_036161605.1 → XP_036017498.1 diacylglycerol lipase-alpha isoform X1

UniProtKB/Swiss-Prot

A8WFL4, Q6WQJ1, Q6ZQ76

UniProtKB/TrEMBL

A4FU75

Conserved Domains (1) summary

cd00519
Location:288 → 529

Lipase_3; Lipase (class 3). Lipases are esterases that can hydrolyze long-chain acyl-triglycerides into di- and monoglycerides, glycerol, and free fatty acids at a water/lipid interface. A typical feature of lipases is "interfacial activation," the process of ...
XM_017318213.2 → XP_017173702.1 diacylglycerol lipase-alpha isoform X1

UniProtKB/Swiss-Prot

A8WFL4, Q6WQJ1, Q6ZQ76

UniProtKB/TrEMBL

A4FU75

Conserved Domains (1) summary

cd00519
Location:288 → 529

Lipase_3; Lipase (class 3). Lipases are esterases that can hydrolyze long-chain acyl-triglycerides into di- and monoglycerides, glycerol, and free fatty acids at a water/lipid interface. A typical feature of lipases is "interfacial activation," the process of ...