Nt5e 5' nucleotidase, ecto [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Nt5eprovided by RGD
Official Full Name: 5' nucleotidase, ectoprovided by RGD
Primary source: RGD:61956
See related: Ensembl:ENSRNOG00000011071 AllianceGenome:RGD:61956
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: Nt5; CD73
Summary: Enables 5'-nucleotidase activity and ferrous iron binding activity. Involved in several processes, including nucleobase-containing small molecule metabolic process; positive regulation of lipid biosynthetic process; and response to aluminum ion. Located in cell surface and synaptic membrane. Biomarker of status epilepticus. Human ortholog(s) of this gene implicated in hereditary arterial and articular multiple calcification syndrome. Orthologous to human NT5E (5'-nucleotidase ecto). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in Liver (RPKM 474.8), Kidney (RPKM 312.5) and 9 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 8q31

Exon count:: 10

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	8	NC_086026.1 (98150925..98195646)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	8	NC_051343.1 (89271046..89314918)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	8	NC_005107.4 (95969002..96012733)

Chromosome 8 - NC_086026.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Immune cell expression patterns of CD39/CD73 ectonucleotidases in rodent models of cardiac arrest and resuscitation.
Title: Immune cell expression patterns of CD39/CD73 ectonucleotidases in rodent models of cardiac arrest and resuscitation.
Expression of functionally distinct ecto-5'-nucleotidase/CD73 glycovariants in reactive astrocytes in experimental autoimmune encephalomyelitis and neuroinflammatory conditions in vitro.
Title: Expression of functionally distinct ecto-5'-nucleotidase/CD73 glycovariants in reactive astrocytes in experimental autoimmune encephalomyelitis and neuroinflammatory conditions in vitro.
Enhanced ATP release and CD73-mediated adenosine formation sustain adenosine A2A receptor over-activation in a rat model of Parkinson's disease.
Title: Enhanced ATP release and CD73-mediated adenosine formation sustain adenosine A(2A) receptor over-activation in a rat model of Parkinson's disease.
in experimental asthma, decreased CD73 expression and activity in airway vagal centers contribute to the augmentation of airway vagal activity
Title: Asthmatic Augmentation of Airway Vagal Activity Involves Decreased Central Expression and Activity of CD73 in Rats.
Studied effect of exercise on memory and cerebral cortex physiology of NTPDase-1 (CD39), 5' nucleotidase ecto (CD73), and adenosine A2a receptor (A2ar).
Title: Physical exercise prevents memory impairment in an animal model of hypertension through modulation of CD39 and CD73 activities and A2A receptor expression.
Our data show CD73 to be a novel regulator of carotid body sensory function and therefore suggest that this enzyme may offer a new target for reducing carotid body activity in selected cardiovascular diseases.
Title: Ecto-5'-nucleotidase (CD73) regulates peripheral chemoreceptor activity and cardiorespiratory responses to hypoxia.
CD73 is an interesting target for glioblastoma treatment and its inhibition may provide new opportunities to improve the treatment of brain tumors.
Title: CD73 Downregulation Decreases In Vitro and In Vivo Glioblastoma Growth.
Prospectively isolated mesenchymal stem/stromal cells are enriched in the CD73 population and exhibit efficacy after differentiation into chondrocytes and transplantation.
Title: Prospectively isolated mesenchymal stem/stromal cells are enriched in the CD73(+) population and exhibit efficacy after transplantation.
Fluvastatin increases adenosine concentrations in the heart ventricles as a result of activation of PKC, mediated by stimulation of alpha1-adrenoceptors, through activation of ecto-5'-nucleotidase.
Title: Fluvastatin, an HMG-CoA reductase inhibitor, facilitate adenosine production in the rat hearts via activation of ecto-5'-nucleotidase.
Epicardium-derived cells from post-myocardial infarction hearts can generate adenosine from both extracellular ATP and NAD via the action of CD73.
Title: CD73-derived adenosine and tenascin-C control cytokine production by epicardium-derived cells formed after myocardial infarction.

Submit: New GeneRIF Correction See all GeneRIFs (47)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

RH133920 (e-PCR)
- UniSTS:217203

BF410734 (e-PCR)
- UniSTS:246932

RH144635 (e-PCR)
- UniSTS:233880

BF399571 (e-PCR)
- UniSTS:247729

Nt5 (e-PCR), detects polymorphism
- UniSTS:144069

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables 5'-deoxynucleotidase activity	IEA Inferred from Electronic Annotation more info
enables 5'-deoxynucleotidase activity	ISO Inferred from Sequence Orthology more info
enables 5'-nucleotidase activity	IBA Inferred from Biological aspect of Ancestor more info
enables 5'-nucleotidase activity	IDA Inferred from Direct Assay more info	PubMed
enables 5'-nucleotidase activity	ISO Inferred from Sequence Orthology more info
enables GMP 5'-nucleotidase activity	IEA Inferred from Electronic Annotation more info
enables GMP 5'-nucleotidase activity	ISO Inferred from Sequence Orthology more info
enables IMP 5'-nucleotidase activity	IEA Inferred from Electronic Annotation more info
enables IMP 5'-nucleotidase activity	ISO Inferred from Sequence Orthology more info
enables XMP 5'-nucleosidase activity	IEA Inferred from Electronic Annotation more info
enables ferrous iron binding	IDA Inferred from Direct Assay more info	PubMed
enables identical protein binding	IEA Inferred from Electronic Annotation more info
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables nucleotidase activity	IEA Inferred from Electronic Annotation more info
enables nucleotide binding	IEA Inferred from Electronic Annotation more info
enables thymidylate 5'-phosphatase activity	IEA Inferred from Electronic Annotation more info
enables thymidylate 5'-phosphatase activity	ISO Inferred from Sequence Orthology more info
enables zinc ion binding	IEA Inferred from Electronic Annotation more info
enables zinc ion binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in ADP catabolic process	IEA Inferred from Electronic Annotation more info
involved_in ADP catabolic process	ISO Inferred from Sequence Orthology more info
involved_in AMP catabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in AMP catabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in AMP catabolic process	IEA Inferred from Electronic Annotation more info
involved_in AMP catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within AMP catabolic process	ISO Inferred from Sequence Orthology more info
involved_in AMP catabolic process	ISO Inferred from Sequence Orthology more info
involved_in ATP metabolic process	IEA Inferred from Electronic Annotation more info
involved_in ATP metabolic process	ISO Inferred from Sequence Orthology more info
involved_in adenosine biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in adenosine biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within adenosine biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in adenosine metabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in calcium ion homeostasis	IEA Inferred from Electronic Annotation more info
involved_in calcium ion homeostasis	ISO Inferred from Sequence Orthology more info
involved_in inhibition of non-skeletal tissue mineralization	IEA Inferred from Electronic Annotation more info
involved_in inhibition of non-skeletal tissue mineralization	ISO Inferred from Sequence Orthology more info
involved_in leukocyte cell-cell adhesion	IEA Inferred from Electronic Annotation more info
involved_in leukocyte cell-cell adhesion	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of inflammatory response	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within negative regulation of inflammatory response	ISO Inferred from Sequence Orthology more info
involved_in nucleotide catabolic process	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of lipid biosynthetic process	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to ATP	IEA Inferred from Electronic Annotation more info
involved_in response to ATP	ISO Inferred from Sequence Orthology more info
involved_in response to aluminum ion	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to inorganic substance	IEA Inferred from Electronic Annotation more info
involved_in response to inorganic substance	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in cell surface	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in external side of plasma membrane	IEA Inferred from Electronic Annotation more info
is_active_in external side of plasma membrane	ISO Inferred from Sequence Orthology more info
located_in membrane	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	IEA Inferred from Electronic Annotation more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
is_active_in plasma membrane	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
located_in side of membrane	IEA Inferred from Electronic Annotation more info
located_in synaptic membrane	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: 5'-nucleotidase

Names: 5'-NT; 5'-deoxynucleotidase; IMP-specific 5'-nucleotidase; ecto-5'-nucleotidase; thymidylate 5'-phosphatase

NP_067587.2

EC 3.1.3.35

EC 3.1.3.5

EC 3.1.3.89

EC 3.1.3.91

EC 3.1.3.99

XP_063122116.1

EC 3.1.3.35

EC 3.1.3.5

EC 3.1.3.89

EC 3.1.3.91

EC 3.1.3.99

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_021576.2 → NP_067587.2 5'-nucleotidase precursor

See identical proteins and their annotated locations for NP_067587.2

Status: PROVISIONAL

Source sequence(s)

BC098665

UniProtKB/Swiss-Prot

P21588

UniProtKB/TrEMBL

F7EWJ6, Q4G083, Q66HL0

Related

ENSRNOP00000015057.3, ENSRNOT00000015057.7

Conserved Domains (3) summary

COG0737
Location:23 → 534

UshA; 2',3'-cyclic-nucleotide 2'-phosphodiesterase/5'- or 3'-nucleotidase, 5'-nucleotidase family [Nucleotide transport and metabolism, Defense mechanisms]

cd07409
Location:31 → 312

MPP_CD73_N; CD73 ecto-5'-nucleotidase and related proteins, N-terminal metallophosphatase domain

pfam02872
Location:341 → 509

5_nucleotid_C; 5'-nucleotidase, C-terminal domain