Pola1 DNA polymerase alpha 1, catalytic subunit [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Pola1provided by RGD
Official Full Name: DNA polymerase alpha 1, catalytic subunitprovided by RGD
Primary source: RGD:621816
See related: Ensembl:ENSRNOG00000013322 AllianceGenome:RGD:621816
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Summary: Enables DNA-directed DNA polymerase activity; double-stranded DNA binding activity; and nucleotide binding activity. Involved in DNA biosynthetic process and DNA replication. Part of alpha DNA polymerase:primase complex. Human ortholog(s) of this gene implicated in Van Esch-O'Driscoll syndrome and X-linked reticulate pigmentary disorder. Orthologous to human POLA1 (DNA polymerase alpha 1, catalytic subunit). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in Thymus (RPKM 160.2), Spleen (RPKM 111.7) and 9 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: Xq22

Exon count:: 38

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	X	NC_086039.1 (62028475..62342455, complement)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	X	NC_051356.1 (58034617..58348612, complement)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	X	NC_005120.4 (62382604..62698830, complement)

Chromosome X - NC_086039.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

YB-1 mediates DNA polymerase alpha gene expression
Title: Transcription factor YB-1 mediates DNA polymerase alpha gene expression.

Submit: New GeneRIF Correction

General gene information

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Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA binding	ISO Inferred from Sequence Orthology more info
enables DNA binding	ISS Inferred from Sequence or Structural Similarity more info
enables DNA replication origin binding	IBA Inferred from Biological aspect of Ancestor more info
enables DNA-directed DNA polymerase activity	IBA Inferred from Biological aspect of Ancestor more info
enables DNA-directed DNA polymerase activity	IDA Inferred from Direct Assay more info	PubMed
enables DNA-directed DNA polymerase activity	IEA Inferred from Electronic Annotation more info
enables DNA-directed DNA polymerase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables DNA-directed DNA polymerase activity	ISO Inferred from Sequence Orthology more info
enables DNA-directed DNA polymerase activity	ISS Inferred from Sequence or Structural Similarity more info
enables chromatin binding	IBA Inferred from Biological aspect of Ancestor more info
enables chromatin binding	ISO Inferred from Sequence Orthology more info
enables chromatin binding	ISS Inferred from Sequence or Structural Similarity more info
enables double-stranded DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables nucleotide binding	IEA Inferred from Electronic Annotation more info
enables nucleotide binding	ISO Inferred from Sequence Orthology more info
enables nucleotide binding	ISS Inferred from Sequence or Structural Similarity more info
enables protein kinase binding	ISO Inferred from Sequence Orthology more info
enables purine nucleotide binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables pyrimidine nucleotide binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables single-stranded DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables zinc ion binding	ISO Inferred from Sequence Orthology more info
enables zinc ion binding	ISS Inferred from Sequence or Structural Similarity more info

Process	Evidence Code	Pubs
involved_in DNA biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in DNA biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
NOT involved_in DNA repair	ISO Inferred from Sequence Orthology more info
involved_in DNA repair	ISO Inferred from Sequence Orthology more info
NOT involved_in DNA repair	ISS Inferred from Sequence or Structural Similarity more info
involved_in DNA replication	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within DNA replication	ISO Inferred from Sequence Orthology more info
involved_in DNA replication	ISO Inferred from Sequence Orthology more info
involved_in DNA replication	ISS Inferred from Sequence or Structural Similarity more info
involved_in DNA replication initiation	ISO Inferred from Sequence Orthology more info
involved_in DNA replication initiation	ISS Inferred from Sequence or Structural Similarity more info
involved_in DNA replication, synthesis of primer	IEA Inferred from Electronic Annotation more info
involved_in DNA replication, synthesis of primer	ISO Inferred from Sequence Orthology more info
involved_in DNA replication, synthesis of primer	ISS Inferred from Sequence or Structural Similarity more info
involved_in DNA strand elongation involved in DNA replication	ISO Inferred from Sequence Orthology more info
involved_in DNA strand elongation involved in DNA replication	ISS Inferred from Sequence or Structural Similarity more info
involved_in DNA synthesis involved in DNA repair	ISO Inferred from Sequence Orthology more info
NOT involved_in DNA synthesis involved in UV-damage excision repair	ISO Inferred from Sequence Orthology more info
NOT involved_in DNA synthesis involved in UV-damage excision repair	ISS Inferred from Sequence or Structural Similarity more info
involved_in double-strand break repair via nonhomologous end joining	ISO Inferred from Sequence Orthology more info
involved_in double-strand break repair via nonhomologous end joining	ISS Inferred from Sequence or Structural Similarity more info
involved_in lagging strand elongation	IBA Inferred from Biological aspect of Ancestor more info
involved_in lagging strand elongation	ISO Inferred from Sequence Orthology more info
involved_in lagging strand elongation	ISS Inferred from Sequence or Structural Similarity more info
involved_in leading strand elongation	IBA Inferred from Biological aspect of Ancestor more info
involved_in leading strand elongation	ISO Inferred from Sequence Orthology more info
involved_in leading strand elongation	ISS Inferred from Sequence or Structural Similarity more info
involved_in mitotic DNA replication initiation	IBA Inferred from Biological aspect of Ancestor more info
involved_in mitotic DNA replication initiation	IEA Inferred from Electronic Annotation more info
NOT involved_in nucleotide-excision repair	ISO Inferred from Sequence Orthology more info
involved_in regulation of type I interferon production	ISO Inferred from Sequence Orthology more info
involved_in regulation of type I interferon production	ISS Inferred from Sequence or Structural Similarity more info

Component	Evidence Code	Pubs
part_of alpha DNA polymerase:primase complex	IBA Inferred from Biological aspect of Ancestor more info
part_of alpha DNA polymerase:primase complex	IC Inferred by Curator more info	PubMed
part_of alpha DNA polymerase:primase complex	IDA Inferred from Direct Assay more info	PubMed
part_of alpha DNA polymerase:primase complex	IEA Inferred from Electronic Annotation more info
part_of alpha DNA polymerase:primase complex	ISO Inferred from Sequence Orthology more info
part_of alpha DNA polymerase:primase complex	ISS Inferred from Sequence or Structural Similarity more info
part_of chromatin	ISO Inferred from Sequence Orthology more info
part_of chromatin	ISS Inferred from Sequence or Structural Similarity more info
located_in cytosol	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in cytosol	ISS Inferred from Sequence or Structural Similarity more info
located_in intracellular non-membrane-bounded organelle	IEA Inferred from Electronic Annotation more info
located_in nuclear envelope	ISO Inferred from Sequence Orthology more info
located_in nuclear envelope	ISS Inferred from Sequence or Structural Similarity more info
located_in nuclear lumen	IEA Inferred from Electronic Annotation more info
located_in nuclear matrix	ISO Inferred from Sequence Orthology more info
located_in nuclear matrix	ISS Inferred from Sequence or Structural Similarity more info
located_in nucleolus	ISO Inferred from Sequence Orthology more info
located_in nucleolus	ISS Inferred from Sequence or Structural Similarity more info
located_in nucleoplasm	IEA Inferred from Electronic Annotation more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	ISS Inferred from Sequence or Structural Similarity more info
located_in nucleus	ISO Inferred from Sequence Orthology more info
located_in nucleus	ISS Inferred from Sequence or Structural Similarity more info

General protein information

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Preferred Names: DNA polymerase alpha catalytic subunit

Names: DNA polymerase alpha catalytic subunit p180; DNA polymerase alpha subunit I; polymerase (DNA directed), alpha 1, catalytic subunit; polymerase (DNA) alpha 1, catalytic subunit

NP_445931.1

EC 2.7.7.7

XP_038956049.1

EC 2.7.7.7

XP_038956050.1

EC 2.7.7.7

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_053479.1 → NP_445931.1 DNA polymerase alpha catalytic subunit

Status: VALIDATED

Source sequence(s)

AI511322, AJ011605, CN543332, FM076387

UniProtKB/Swiss-Prot

O89042

Related

ENSRNOP00000068341.2, ENSRNOT00000075997.3

Conserved Domains (5) summary

cd05776
Location:542 → 774

DNA_polB_alpha_exo; inactive DEDDy 3'-5' exonuclease domain of eukaryotic DNA polymerase alpha, a family-B DNA polymerase

cd05532
Location:839 → 1249

POLBc_alpha; DNA polymerase type-B alpha subfamily catalytic domain. Three DNA-dependent DNA polymerases type B (alpha, delta, and epsilon) have been identified as essential for nuclear DNA replication in eukaryotes. DNA polymerase (Pol) alpha is almost exclusively ...

TIGR00592
Location:56 → 1232

pol2; DNA polymerase (pol2)

pfam08996
Location:1272 → 1461

zf-DNA_Pol; DNA Polymerase alpha zinc finger

pfam12254
Location:41 → 101

DNA_pol_alpha_N; DNA polymerase alpha subunit p180 N terminal

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086039.1 Reference GRCr8

Range

62028475..62342455 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_039100121.2 → XP_038956049.1 DNA polymerase alpha catalytic subunit isoform X1

Conserved Domains (1) summary

TIGR00592
Location:56 → 989

pol2; DNA polymerase (pol2)
XM_039100122.1 → XP_038956050.1 DNA polymerase alpha catalytic subunit isoform X2

Conserved Domains (1) summary

TIGR00592
Location:56 → 857

pol2; DNA polymerase (pol2)