Ppp1r9b protein phosphatase 1, regulatory subunit 9B [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Ppp1r9bprovided by MGI
Official Full Name: protein phosphatase 1, regulatory subunit 9Bprovided by MGI
Primary source: MGI:MGI:2387581
See related: Ensembl:ENSMUSG00000038976 AllianceGenome:MGI:2387581
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: SPL; Spn
Summary: Predicted to enable several functions, including D2 dopamine receptor binding activity; enzyme binding activity; and protein C-terminus binding activity. Involved in cellular response to morphine and regulation of opioid receptor signaling pathway. Acts upstream of or within several processes, including actin filament organization; calcium-mediated signaling; and dendrite development. Located in cortical actin cytoskeleton and filopodium. Is expressed in several structures, including alimentary system; brain; cardiovascular system; genitourinary system; and immune system. Orthologous to human PPP1R9B (protein phosphatase 1 regulatory subunit 9B). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Ubiquitous expression in frontal lobe adult (RPKM 75.4), cortex adult (RPKM 71.9) and 27 other tissues See more
Orthologs: human all
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Genomic context

Location:: 11 D; 11 59.01 cM

Exon count:: 10

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	11	NC_000077.7 (94882038..94897724)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	11	NC_000077.6 (94991212..95006898)

Chromosome 11 - NC_000077.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Spinophilin Limits Metabotropic Glutamate Receptor 5 Scaffolding to the Postsynaptic Density and Cell Type Specifically Mediates Excessive Grooming.
Title: Spinophilin Limits Metabotropic Glutamate Receptor 5 Scaffolding to the Postsynaptic Density and Cell Type Specifically Mediates Excessive Grooming.
Four-month treadmill exercise prevents the decline in spatial learning and memory abilities and the loss of spinophilin-immunoreactive puncta in the hippocampus of APP/PS1 transgenic mice.
Title: Four-month treadmill exercise prevents the decline in spatial learning and memory abilities and the loss of spinophilin-immunoreactive puncta in the hippocampus of APP/PS1 transgenic mice.
spinophilin/PP1 complex negatively controlled TRPV1 function in DRG neurons
Title: Spinophilin negatively controlled the function of transient receptor potential vanilloid 1 in dorsal root ganglia neurons of mice.
spinophilin fulfills an essential role in cocaine-induced behavioral sensitization, likely via ERK1/2 phosphorylation and induction of c-Fos and FosB in the striatum.
Title: Neuronal scaffolding protein spinophilin is integral for cocaine-induced behavioral sensitization and ERK1/2 activation.
two homologs neurabin and spinophilin play important yet distinct roles in the regulation of anxiety- and depression-like behaviors in an age-dependent manner.
Title: Age-dependent differential regulation of anxiety- and depression-related behaviors by neurabin and spinophilin.
results indicate that spinophilin plays an important role in regulating the activity of Group I mGluRs as well as their influence on synaptic activity.
Title: Role of Spinophilin in Group I Metabotropic Glutamate Receptor Endocytosis, Signaling, and Synaptic Plasticity.
COPS5 overexpression reduced spinophilin in both the cortex (19%, p < 0.05) and the hippocampus (20%, p < 0.05), leading to significant deficits in learning and memory skills
Title: COPS5 protein overexpression increases amyloid plaque burden, decreases spinophilin-immunoreactive puncta, and exacerbates learning and memory deficits in the mouse brain.
RanBP9 overexpression in transgenic mice led to striking reduction in the levels of spinophilin.
Title: RanBP9 aggravates synaptic damage in the mouse brain and is inversely correlated to spinophilin levels in Alzheimer's brain synaptosomes.
Canonical betaAR-mediated signaling coupled to PKA activation results in phosphorylation of spinophilin, disrupting its interaction with alpha2AARs and accelerating alpha2AAR endocytic responses.
Title: Cross-talk from β-adrenergic receptors modulates α2A-adrenergic receptor endocytosis in sympathetic neurons via protein kinase A and spinophilin.
Findings support the concept that the inhibitory effects of SPL on M3R activity are mediated by RGS4.
Title: Spinophilin as a novel regulator of M3 muscarinic receptor-mediated insulin release in vitro and in vivo.

Submit: New GeneRIF Correction See all GeneRIFs (27)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (1)
Targeted (3) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables D2 dopamine receptor binding	ISO Inferred from Sequence Orthology more info
enables actin binding	ISO Inferred from Sequence Orthology more info
enables actin binding	TAS Traceable Author Statement more info	PubMed
enables actin filament binding	IBA Inferred from Biological aspect of Ancestor more info
enables actin filament binding	ISO Inferred from Sequence Orthology more info
enables kinase binding	ISO Inferred from Sequence Orthology more info
enables nucleic acid binding	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase activity	ISO Inferred from Sequence Orthology more info
enables protein phosphatase 1 binding	ISO Inferred from Sequence Orthology more info
enables protein phosphatase inhibitor activity	ISO Inferred from Sequence Orthology more info
enables protein-containing complex binding	ISO Inferred from Sequence Orthology more info
enables transmembrane transporter binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within actin cytoskeleton organization	TAS Traceable Author Statement more info	PubMed
involved_in actin filament organization	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within actin filament organization	IPI Inferred from Physical Interaction more info	PubMed
involved_in calcium-mediated signaling	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within calcium-mediated signaling	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within cell differentiation	IEA Inferred from Electronic Annotation more info
involved_in cell migration	ISO Inferred from Sequence Orthology more info
involved_in cellular response to morphine	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within dendrite development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in filopodium assembly	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within multicellular organism development	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of cell growth	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within nervous system development	IEA Inferred from Electronic Annotation more info
involved_in neuron projection development	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of protein localization	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of protein localization to actin cortical patch	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of protein localization to plasma membrane	ISO Inferred from Sequence Orthology more info
involved_in protein localization to actin cytoskeleton	ISO Inferred from Sequence Orthology more info
involved_in protein localization to cell periphery	ISO Inferred from Sequence Orthology more info
involved_in regulation of opioid receptor signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of postsynapse assembly	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of postsynapse assembly	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within regulation of protein phosphorylation	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in actin cytoskeleton	IBA Inferred from Biological aspect of Ancestor more info
located_in actin cytoskeleton	ISO Inferred from Sequence Orthology more info
located_in anchoring junction	IEA Inferred from Electronic Annotation more info
located_in cell projection	IEA Inferred from Electronic Annotation more info
located_in cortical actin cytoskeleton	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytoplasmic side of dendritic spine plasma membrane	ISO Inferred from Sequence Orthology more info
located_in cytoskeleton	IEA Inferred from Electronic Annotation more info
is_active_in dendrite	IBA Inferred from Biological aspect of Ancestor more info
located_in dendritic spine	ISO Inferred from Sequence Orthology more info
located_in dendritic spine head	ISO Inferred from Sequence Orthology more info
located_in dendritic spine membrane	ISO Inferred from Sequence Orthology more info
located_in dendritic spine neck	ISO Inferred from Sequence Orthology more info
located_in filopodium	IDA Inferred from Direct Assay more info	PubMed
located_in filopodium	ISO Inferred from Sequence Orthology more info
located_in growth cone	ISO Inferred from Sequence Orthology more info
located_in lamellipodium	ISO Inferred from Sequence Orthology more info
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in neuronal cell body	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
located_in nucleus	IEA Inferred from Electronic Annotation more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
is_active_in postsynaptic density	IBA Inferred from Biological aspect of Ancestor more info
located_in ruffle membrane	ISO Inferred from Sequence Orthology more info
located_in synapse	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: neurabin-2

Names: neurabin-II; spinophilin

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_172261.3 → NP_758465.2 neurabin-2

See identical proteins and their annotated locations for NP_758465.2

Status: VALIDATED

Source sequence(s)

AI841686, AY508450

Consensus CDS

CCDS25268.1

UniProtKB/Swiss-Prot

Q6R891, Q8K0X7

Related

ENSMUSP00000041732.6, ENSMUST00000038696.12

Conserved Domains (3) summary

smart00228
Location:493 → 582

PDZ; Domain present in PSD-95, Dlg, and ZO-1/2

pfam07106
Location:682 → 763

TBPIP; Tat binding protein 1(TBP-1)-interacting protein (TBPIP)

cl23943
Location:745 → 811

PCRF; PCRF domain