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    Tk1 thymidine kinase 1 [ Mus musculus (house mouse) ]

    Gene ID: 21877, updated on 9-May-2024

    Summary

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    Official Symbol
    Tk1provided by MGI
    Official Full Name
    thymidine kinase 1provided by MGI
    Primary source
    MGI:MGI:98763
    See related
    Ensembl:ENSMUSG00000025574 AllianceGenome:MGI:98763
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Mus musculus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
    Also known as
    Tk-1; Tk1a; Tk1b; D530002A18Rik
    Summary
    Enables thymidine kinase activity. Predicted to be involved in protein homotetramerization and thymidine metabolic process. Predicted to act upstream of or within DNA biosynthetic process. Predicted to be located in cytosol. Is expressed in several structures, including alimentary system; central nervous system; genitourinary system; heart; and hemolymphoid system. Orthologous to human TK1 (thymidine kinase 1). [provided by Alliance of Genome Resources, Apr 2022]
    Expression
    Broad expression in liver E14.5 (RPKM 50.7), liver E14 (RPKM 47.2) and 19 other tissues See more
    Orthologs
    human all
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    Genomic context

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    See Tk1 in Genome Data Viewer
    Location:
    11 E2; 11 82.96 cM
    Exon count:
    9
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCm39 (GCF_000001635.27) 11 NC_000077.7 (117706345..117716913, complement)
    108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 11 NC_000077.6 (117815519..117826087, complement)

    Chromosome 11 - NC_000077.7Genomic Context describing neighboring genes Neighboring gene RIKEN cDNA 6030468B19 gene Neighboring gene STARR-positive B cell enhancer ABC_E5305 Neighboring gene STARR-positive B cell enhancer ABC_E5306 Neighboring gene synaptogyrin 2 Neighboring gene STARR-positive B cell enhancer ABC_E11501 Neighboring gene STARR-positive B cell enhancer ABC_E4020 Neighboring gene CapStarr-seq enhancer MGSCv37_chr11:117693509-117693710 Neighboring gene arylformamidase Neighboring gene CapStarr-seq enhancer MGSCv37_chr11:117693767-117693876 Neighboring gene CapStarr-seq enhancer MGSCv37_chr11:117710178-117710379 Neighboring gene baculoviral IAP repeat-containing 5 Neighboring gene VISTA enhancer mm780 Neighboring gene transmembrane protein 235

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: Mouse ENCODE transcriptome data
    • Description: RNA profiling data sets generated by the Mouse ENCODE project.
    • BioProject: PRJNA66167
    • Publication: PMID 25409824
    • Analysis date: n/a

    Bibliography

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    Related articles in PubMed

    1. Micronucleated erythrocyte frequency in control and azidothymidine-treated Tk+/+, Tk+/- and Tk-/- mice. Dobrovolsky VN, et al. Mutat Res, 2005 Mar 1. PMID 15708581
    2. Analysis of mutations in the Tk gene of Tk(+/-) mice treated as neonates with 3'-azido-3'-deoxythymidine (AZT). Mittelstaedt RA, et al. Mutat Res, 2004 Mar 22. PMID 15013700
    3. Mice deficient for cytosolic thymidine kinase gene develop fatal kidney disease. Dobrovolsky VN, et al. Mol Genet Metab, 2003 Jan. PMID 12559842
    4. Mutant frequencies and loss of heterozygosity induced by N-ethyl-N-nitrosourea in the thymidine kinase gene of L5178Y/TK(+/-)-3.7.2C mouse lymphoma cells. Chen T, et al. Mutagenesis, 2002 Mar. PMID 11880538
    5. Development of a novel mouse tk+/- embryonic stem cell line for use in mutagenicity studies. Dobrovolsky VN, et al. Environ Mol Mutagen, 1996. PMID 8991081

    See all (81) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Transcription factor Dp-1 knockdown downregulates thymidine kinase 1 expression to protect against proliferation and epithelial-mesenchymal transition in cervical cancer.
      Title: Transcription factor Dp-1 knockdown downregulates thymidine kinase 1 expression to protect against proliferation and epithelial-mesenchymal transition in cervical cancer.
    2. The down-regulation of Tk1 activity unmasks Tk2 deficiency in Tk2-/- mice and correlates with the onset of mtDNA depletion in the brain and the heart.
      Title: Onset and organ specificity of Tk2 deficiency depends on Tk1 down-regulation and transcriptional compensation.
    3. blood cells are less dependent on mitochondrial Tk2 compared with several other tissues; these cells can synthesize deoxyribonucleotides required for mtDNA replication by alternative pathways such as phosphorylation of thymidine by cytosolic Tk1
      Title: Hematopoiesis in the thymidine kinase 2 deficient mouse model of mitochondrial DNA depletion syndrome.
    4. Mutations in the TK1 gene were studied in neonatal transgenic mice treated with AZT.
      Title: Analysis of mutations in the Tk gene of Tk(+/-) mice treated as neonates with 3'-azido-3'-deoxythymidine (AZT).
    5. the small colony mutant phenotype in L5178Y lymphoma is not caused by deletion of both Trp53 and thymidine kinase 1
      Title: Loss of P53 heterozygosity is not responsible for the small colony thymidine kinase mutant phenotype in L5178Y mouse lymphoma cells.
    6. Alterations in Tk KO mice indicate that the pyrimidine nucleotide salvage pathway is indispensable in vivo.
      Title: Mice deficient for cytosolic thymidine kinase gene develop fatal kidney disease.
    Submit: New GeneRIF Correction

    Variation

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    Alleles

    Alleles of this type are documented at Mouse Genome Informatics  (MGI)
    • Endonuclease-mediated (3) 
    • Gene trapped (1) 
    • Targeted (1)  1 citation

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Markers

    Homology

    Gene Ontology Provided by MGI

    Function Evidence Code Pubs
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables identical protein binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables kinase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables nucleotide binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables thymidine kinase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables thymidine kinase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables thymidine kinase activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables transferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables zinc ion binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    Process Evidence Code Pubs
    acts_upstream_of_or_within DNA biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in DNA synthesis involved in mitotic DNA replication ISO
    Inferred from Sequence Orthology
    more info
    		  
    acts_upstream_of_or_within deoxyribonucleoside monophosphate biosynthetic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within nucleotide biosynthetic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within phosphorylation IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in protein homotetramerization ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in thymidine biosynthetic process ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in thymidine metabolic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in thymidine metabolic process ISO
    Inferred from Sequence Orthology
    more info
    		  
    Component Evidence Code Pubs
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in cytosol TAS
    Traceable Author Statement
    more info
    		  

    General protein information

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    Preferred Names
    thymidine kinase, cytosolic
    NP_001258658.1
    NP_033413.2
    XP_006533212.1
    XP_036012517.1

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001271729.1 → NP_001258658.1  thymidine kinase, cytosolic isoform 2

      See identical proteins and their annotated locations for NP_001258658.1

      Status: VALIDATED

      Description
      Transcript Variant: This variant (2) lacks an alternate coding exon compared to variant 1, that causes a frameshift and use of a downstream translation start site compared to variant 1. The resulting isoform (2) is shorter at the N-terminus compared to isoform 1.
      Source sequence(s)
      AA047942, AK139244, BY773001, CF732600
      UniProtKB/Swiss-Prot
      P04184
      Conserved Domains (1) summary
      cl23762
      Location:1 → 161
      TK; Thymidine kinase

    2. NM_009387.2 → NP_033413.2  thymidine kinase, cytosolic isoform 1

      See identical proteins and their annotated locations for NP_033413.2

      Status: VALIDATED

      Description
      Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).
      Source sequence(s)
      AA047942, AK139244, BY773001
      Consensus CDS
      CCDS25692.1
      UniProtKB/Swiss-Prot
      P04184, Q58EU0, Q8C3P8, Q921J8
      UniProtKB/TrEMBL
      A0A1S6GWJ3
      Related
      ENSMUSP00000026661.4, ENSMUST00000026661.4
      Conserved Domains (1) summary
      cl23762
      Location:18 → 197
      TK; Thymidine kinase

    RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCm39 C57BL/6J

    Genomic

    1. NC_000077.7 Reference GRCm39 C57BL/6J

      Range
      117706345..117716913 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_036156624.1 → XP_036012517.1  thymidine kinase, cytosolic isoform X2

      Conserved Domains (1) summary
      cl23762
      Location:1 → 161
      TK; Thymidine kinase

    2. XM_006533149.3 → XP_006533212.1  thymidine kinase, cytosolic isoform X1

      Conserved Domains (1) summary
      cl23762
      Location:41 → 199
      TK; Thymidine kinase

    RNA

    1. XR_004936934.1 RNA Sequence

    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P04184.3 GenPept UniProtKB/Swiss-Prot:P04184

    Gene LinkOut

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