Rhbg Rhesus blood group-associated B glycoprotein [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Rhbgprovided by MGI
Official Full Name: Rhesus blood group-associated B glycoproteinprovided by MGI
Primary source: MGI:MGI:1927379
See related: Ensembl:ENSMUSG00000104445 AllianceGenome:MGI:1927379
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Summary: Enables ammonium transmembrane transporter activity. Involved in ammonium transmembrane transport. Located in basolateral plasma membrane. Is expressed in genitourinary system. Orthologous to human RHBG (Rh family B glycoprotein). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in kidney adult (RPKM 38.4), large intestine adult (RPKM 18.5) and 10 other tissues See more
Orthologs: human all
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Genomic context

Location:: 3 F1; 3 38.78 cM

Exon count:: 10

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	3	NC_000069.7 (88150181..88162046, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	3	NC_000069.6 (88242874..88254739, complement)

Chromosome 3 - NC_000069.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Utilizing a structural homology analysis to AmtB, we have identified residues in Rhbg that are implicated in ion transport function. We introduced mutations into these residues and evaluated their function using measurements of intracellular pH (pHi), surface pH (pHs), and whole cell currents (I) that can distinguish between NH3 and NH4+ signals. Our results identified critical amino acids that affect transport.
Title: Structural determinants of NH3 and NH4+ transport by mouse Rhbg, a renal Rh glycoprotein.
We characterized ammonia and ammonium (NH3/NH4 (+)) transport by the rhesus-associated (Rh) glycoproteins RhAG, Rhbg, and Rhcg expressed in Xenopus oocytes. We used ion-selective microelectrodes and two-electrode voltage clamp to measure changes in intracellular pH, surface pH, and whole cell currents induced by NH3/NH4 (+) and methyl amine/ammonium (MA/MA(+)).
Title: Mechanisms of ammonia and ammonium transport by rhesus-associated glycoproteins.
Rh B Glycoprotein (Rhbg) and Rh C Glycoprotein (Rhcg) are identified as ammonia transporters. They are expressed in specific cell populations and membrane domains in distal renal epithelial cells, where they facilitate ammonia secretion.
Title: Ammonia transport in the kidney by Rhesus glycoproteins.
Rhbg is critically important in the renal response to metabolic acidosis. in mice with intact Rhbg expression, metabolic acidosis does not induce maximal changes in PEPCK, NHE-3, and GS expression despite the presence of persistent metabolic acidosis.
Title: Effect of collecting duct-specific deletion of both Rh B Glycoprotein (Rhbg) and Rh C Glycoprotein (Rhcg) on renal response to metabolic acidosis.
highly expressed in specific cells in the male reproductive tract
Title: Expression of the rhesus glycoproteins, ammonia transporter family members, RHCG and RHBG in male reproductive organs.
It was concluded that hypokalemia increases Rhbg expression in intercalated cells in the cortex and outer medulla and that intercalated cell Rhbg expression is necessary for the normal increase in renal ammonia excretion in response to hypokalemia.
Title: Intercalated cell-specific Rh B glycoprotein deletion diminishes renal ammonia excretion response to hypokalemia.
These data indicate that transport of NH(4)(+) and methyl ammonium (+) by Rhbg is pH sensitive.
Title: pH sensitivity of ammonium transport by Rhbg.
Rhbg in connecting segment and principal cells may contribute to renal ammonia excretion
Title: Role of the Rhesus glycoprotein, Rh B glycoprotein, in renal ammonia excretion.
Rhbg and Rhcg may contribute to bronchial epithelial cell ammonia metabolism and suggest that they do not contribute to pulmonary carbon dioxide transport.
Title: Expression of the gas-transporting proteins, Rh B glycoprotein and Rh C glycoprotein, in the murine lung.
Characterization of the role of Rhbg in ammonium ion transport using voltage clamp techniques.
Title: Characteristics of renal Rhbg as an NH4(+) transporter.

Submit: New GeneRIF Correction See all GeneRIFs (21)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (2)
Gene trapped (1)
Targeted (2) 1 citation

General gene information

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Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ammonium transmembrane transporter activity	IBA Inferred from Biological aspect of Ancestor more info
enables ammonium transmembrane transporter activity	IDA Inferred from Direct Assay more info	PubMed
enables ammonium transmembrane transporter activity	ISO Inferred from Sequence Orthology more info
enables ankyrin binding	ISO Inferred from Sequence Orthology more info
enables carbon dioxide transmembrane transporter activity	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in ammonium homeostasis	IBA Inferred from Biological aspect of Ancestor more info
involved_in ammonium transmembrane transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in ammonium transmembrane transport	IDA Inferred from Direct Assay more info	PubMed
involved_in ammonium transmembrane transport	ISO Inferred from Sequence Orthology more info
involved_in transepithelial ammonium transport	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in basolateral plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in basolateral plasma membrane	ISO Inferred from Sequence Orthology more info
located_in membrane	IDA Inferred from Direct Assay more info	PubMed
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	ISS Inferred from Sequence or Structural Similarity more info	PubMed
located_in spectrin-associated cytoskeleton	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: ammonium transporter Rh type B

Names: Rh type B glycoprotein; rh family type B glycoprotein; rhesus blood group family type B glycoprotein

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_021375.3 → NP_067350.2 ammonium transporter Rh type B

See identical proteins and their annotated locations for NP_067350.2

Status: PROVISIONAL

Source sequence(s)

AK081882

Consensus CDS

CCDS17466.1

UniProtKB/Swiss-Prot

Q8BUX5, Q9QXP1

UniProtKB/TrEMBL

E9Q2A2

Related

ENSMUSP00000130767.2, ENSMUST00000171887.4

Conserved Domains (1) summary

cl03012
Location:58 → 403

Ammonium_transp; Ammonium Transporter Family

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000069.7 Reference GRCm39 C57BL/6J

Range

88150181..88162046 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006501826.4 → XP_006501889.1 ammonium transporter Rh type B isoform X1

UniProtKB/TrEMBL

E9Q2A2

Conserved Domains (1) summary

cl03012
Location:58 → 362

Ammonium_transp; Ammonium Transporter Family