BRE5 Bre5p [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: BRE5
Gene description: Bre5p
Primary source: FungiDB:YNR051C
Locus tag: YNR051C
See related: SGD:S000005334; AllianceGenome:SGD:S000005334
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables mRNA binding activity. Involved in several processes, including protein retention in Golgi apparatus; regulation of vesicle-mediated transport; and ribophagy. Located in P-body. Part of Ubp3-Bre5 deubiquitination complex. Orthologous to human G3BP1 (G3BP stress granule assembly factor 1). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See BRE5 in Genome Data Viewer

Location:: chromosome: XIV

Exon count:: 1

Sequence:: Chromosome: XIV; NC_001146.8 (716780..718327, complement)

Chromosome XIV - NC_001146.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001146.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

A novel mechanism for the retention of Golgi membrane proteins mediated by the Bre5p/Ubp3p deubiquitinase complex.
Title: A novel mechanism for the retention of Golgi membrane proteins mediated by the Bre5p/Ubp3p deubiquitinase complex.
Here, the authors identify a site of ubiquitination (K1246) in the catalytic subunit of RNAPII close to the DNA entry path. Ubiquitination was increased in the absence of the Bre5-Ubp3 ubiquitin protease complex.
Title: RNA polymerase II stalling at pre-mRNA splice sites is enforced by ubiquitination of the catalytic subunit.
show that mature ribosomes are rapidly degraded by autophagy upon nutrient starvation in Saccharomyces cerevisiae, and that selective degradation of ribosomes requires catalytic activity of the Ubp3p/Bre5p ubiquitin protease.
Title: Mature ribosomes are selectively degraded upon starvation by an autophagy pathway requiring the Ubp3p/Bre5p ubiquitin protease.
Ubp3/Bre5 functions antagonistically with Bul1 in DNA damage responses, but not in its other cellular functions
Title: The Bre5/Ubp3 ubiquitin protease complex from budding yeast contributes to the cellular response to DNA damage.
New insights into Ubp3 recognition by Bre5 and into protein recognition by NTF2-like domains.
Title: Molecular basis for bre5 cofactor recognition by the ubp3 deubiquitylating enzyme.
analysis of Ubp3 deubiquitinating enzyme interaction with its Bre5 cofactor
Title: Structural basis for interaction between the Ubp3 deubiquitinating enzyme and its Bre5 cofactor.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables RNA binding	IEA Inferred from Electronic Annotation more info
enables mRNA binding	HDA more info	PubMed
enables mRNA binding	IBA Inferred from Biological aspect of Ancestor more info

Process	Evidence Code	Pubs
involved_in cellular localization	IEA Inferred from Electronic Annotation more info
involved_in protein deubiquitination	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein deubiquitination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein retention in Golgi apparatus	IGI Inferred from Genetic Interaction more info	PubMed
involved_in protein retention in Golgi apparatus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of ER to Golgi vesicle-mediated transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of retrograde vesicle-mediated transport, Golgi to ER	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in ribophagy	IBA Inferred from Biological aspect of Ancestor more info
involved_in ribophagy	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in P-body	IDA Inferred from Direct Assay more info	PubMed
part_of Ubp3-Bre5 deubiquitination complex	IBA Inferred from Biological aspect of Ancestor more info
part_of Ubp3-Bre5 deubiquitination complex	IPI Inferred from Physical Interaction more info	PubMed
located_in cytoplasm	HDA more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytosol	HDA more info	PubMed
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
part_of ribonucleoprotein complex	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: Bre5p

NP_014449.1

Ubiquitin protease cofactor; forms deubiquitination complex with Ubp3p that coregulates anterograde and retrograde transport between the ER and Golgi compartments, deubiquitinating COPII and COPI vesicle coat constituents, Sec23p and Sec27p; involved along with Ubp3p in the steady-state retention of Golgi membrane proteins, such as glycosyltransferases; null is sensitive to brefeldin A

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001146.8 Reference assembly

Range

716780..718327 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001183228.1 → NP_014449.1 TPA: Bre5p [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_014449.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W1M6, P53741

UniProtKB/TrEMBL

A0A8H4F991, B5VR76, C7GLK6, N1NXU1

Conserved Domains (2) summary

cd00780
Location:4 → 142

NTF2; Nuclear transport factor 2 (NTF2) domain plays an important role in the trafficking of macromolecules, ions and small molecules between the cytoplasm and nucleus. This bi-directional transport of macromolecules across the nuclear envelope requires many ...

cl17169
Location:427 → 483

RRM_SF; RNA recognition motif (RRM) superfamily