Nln neurolysin [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Nlnprovided by RGD
Official Full Name: neurolysinprovided by RGD
Primary source: RGD:621518
See related: Ensembl:ENSRNOG00000011561 AllianceGenome:RGD:621518
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Summary: Enables metalloendopeptidase activity and oligopeptidase activity. Involved in proteolysis. Located in cytosol and mitochondrial intermembrane space. Orthologous to human NLN (neurolysin). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in Muscle (RPKM 156.9), Spleen (RPKM 145.0) and 9 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 2q13

Exon count:: 18

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	2	NC_086020.1 (36867436..36966749, complement)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	2	NC_051337.1 (35133634..35232927, complement)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	2	NC_005101.4 (34086970..34185835, complement)

Chromosome 2 - NC_086020.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Data indicate that the inhibitor binds away from the catalytic site of neuropeptidase neurolysin.
Title: Allosteric inhibition of the neuropeptidase neurolysin.
Neurolysin was identified as a non-AT1 and non-AT2 binding site for Angiotensin II, suggesting that neurolysin may significantly affect the activity of the renin-angiotensin system.
Title: Pharmacological characterization of a novel non-AT1, non-AT2 angiotensin binding site identified as neurolysin.
Ciliary epithelium expresses neurolysin.
Title: Molecular analysis of neurolysin expression in the rat and bovine ciliary body.
this is the first evidence of an in vivo role for EP 24.16 in brain function, specifically in regulating alterations in microvessel permeability induced by exogenous bradykinin
Title: Metalloendopeptidases EC 3.4.24.15/16 regulate bradykinin activity in the cerebral microvasculature.
EP24.16 activity in vascular endothelial cells may play an important role in the degradation of bradykinin and/or other peptides in the circulation
Title: Endopeptidases 3.4.24.15 and 24.16 in endothelial cells: potential role in vasoactive peptide metabolism.
Data report mapping of the positions of the 224 surface residues that differ in sequence between thimet oligopeptidase and neurolysin onto the surface of the neurolysin crystal structure.
Title: Mapping sequence differences between thimet oligopeptidase and neurolysin implicates key residues in substrate recognition.
Taken together, these data demonstrate that EP24.15/16 modulate intracellular, peptidergic signaling cascades through B2R in a physiologically relevant nociceptive system.
Title: Modulation of bradykinin signaling by EP24.15 and EP24.16 in cultured trigeminal ganglia.

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General gene information

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Markers

BM382907 (e-PCR)
- UniSTS:260959

D2Mgh1 (e-PCR)
- UniSTS:118648

RH132947 (e-PCR)
- UniSTS:216230

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables metalloendopeptidase activity	IBA Inferred from Biological aspect of Ancestor more info
enables metalloendopeptidase activity	IDA Inferred from Direct Assay more info	PubMed
enables metalloendopeptidase activity	IEA Inferred from Electronic Annotation more info
enables oligopeptidase activity	IDA Inferred from Direct Assay more info	PubMed
enables peptidase activity	IDA Inferred from Direct Assay more info	PubMed
enables peptidase activity	ISO Inferred from Sequence Orthology more info
enables peptide binding	IEA Inferred from Electronic Annotation more info
enables peptide binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in peptide catabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in peptide metabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein catabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in proteolysis	IBA Inferred from Biological aspect of Ancestor more info
involved_in proteolysis	IEA Inferred from Electronic Annotation more info
involved_in regulation of gluconeogenesis	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within regulation of gluconeogenesis	ISO Inferred from Sequence Orthology more info
involved_in regulation of skeletal muscle fiber differentiation	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within regulation of skeletal muscle fiber differentiation	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in cytosol	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
is_active_in mitochondrial intermembrane space	IBA Inferred from Biological aspect of Ancestor more info
located_in mitochondrial intermembrane space	IEA Inferred from Electronic Annotation more info
located_in mitochondrion	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	IEA Inferred from Electronic Annotation more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: neurolysin, mitochondrial

Names: MEP; microsomal endopeptidase; mitochondrial oligopeptidase M; neurolysin (metallopeptidase M3 family); neurotensin endopeptidase

NP_446422.2

EC 3.4.24.16

XP_006231942.1

EC 3.4.24.16

XP_038957511.1

EC 3.4.24.16

XP_038957512.1

EC 3.4.24.16

XP_038957513.1

EC 3.4.24.16

XP_063137241.1

EC 3.4.24.16

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_053970.2 → NP_446422.2 neurolysin, mitochondrial precursor

See identical proteins and their annotated locations for NP_446422.2

Status: PROVISIONAL

Source sequence(s)

BC072687

UniProtKB/Swiss-Prot

P42676, Q6GQQ4

UniProtKB/TrEMBL

A0A0G2JSY3, A6I5E6

Related

ENSRNOP00000059652.1, ENSRNOT00000066925.2

Conserved Domains (2) summary

cd06455
Location:58 → 699

M3A_TOP; Peptidase M3 Thimet oligopeptidase (TOP) also includes neurolysin

COG0339
Location:54 → 703

Dcp; Zn-dependent oligopeptidase [Posttranslational modification, protein turnover, chaperones]

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086020.1 Reference GRCr8

Range

36867436..36966749 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_039101583.2 → XP_038957511.1 neurolysin, mitochondrial isoform X1

UniProtKB/Swiss-Prot

P42676, Q6GQQ4

Conserved Domains (1) summary

cd09605
Location:58 → 663

M3A; Peptidase M3A family includes thimet oligopeptidase, dipeptidyl carboxypeptidase and mitochondrial intermediate peptidase
XM_039101584.2 → XP_038957512.1 neurolysin, mitochondrial isoform X2

UniProtKB/TrEMBL

A0A0G2JVK4

Conserved Domains (1) summary

cd09605
Location:35 → 640

M3A; Peptidase M3A family includes thimet oligopeptidase, dipeptidyl carboxypeptidase and mitochondrial intermediate peptidase
XM_006231880.5 → XP_006231942.1 neurolysin, mitochondrial isoform X3

UniProtKB/Swiss-Prot

P42676, Q6GQQ4

Conserved Domains (1) summary

cd06455
Location:58 → 661

M3A_TOP; Peptidase M3 Thimet oligopeptidase (TOP) also includes neurolysin
XM_063281171.1 → XP_063137241.1 neurolysin, mitochondrial isoform X5
XM_039101585.2 → XP_038957513.1 neurolysin, mitochondrial isoform X4

Conserved Domains (1) summary

cd06455
Location:58 → 509

M3A_TOP; Peptidase M3 thimet oligopeptidase (TOP), also includes neurolysin