Eef2k eukaryotic elongation factor-2 kinase [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Eef2kprovided by RGD
Official Full Name: eukaryotic elongation factor-2 kinaseprovided by RGD
Primary source: RGD:2538
See related: Ensembl:ENSRNOG00000016448 AllianceGenome:RGD:2538
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: SMEF2K
Summary: Enables elongation factor-2 kinase activity. Involved in several processes, including cellular response to anoxia; positive regulation of cellular component organization; and response to peptide hormone. Located in dendritic spine and postsynaptic density. Orthologous to human EEF2K (eukaryotic elongation factor 2 kinase). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in Muscle (RPKM 171.7), Heart (RPKM 69.4) and 9 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 1q36

Exon count:: 21

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	1	NC_086019.1 (184824420..184888048)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	1	NC_051336.1 (175393119..175456756)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	1	NC_005100.4 (190780211..190839926)

Chromosome 1 - NC_086019.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Eukaryotic elongation factor 2 kinase inhibitor, A484954 inhibits perivascular sympathetic nerve stimulation-induced vasoconstriction in isolated renal artery.
Title: Eukaryotic elongation factor 2 kinase inhibitor, A484954 inhibits perivascular sympathetic nerve stimulation-induced vasoconstriction in isolated renal artery.
Age-dependent increase in activity of eukaryotic elongation factor 2 kinase in mesenteric arteries from spontaneously hypertensive rats.
Title: Age-dependent increase in activity of eukaryotic elongation factor 2 kinase in mesenteric arteries from spontaneously hypertensive rats.
NMDA-induced nitric oxide generation and CREB activation in central nervous system is dependent on eukaryotic elongation factor 2 kinase.
Title: NMDA-induced nitric oxide generation and CREB activation in central nervous system is dependent on eukaryotic elongation factor 2 kinase.
EEF2K/eEF2 axis under GD condition mediates the inhibition of apoptotic H9c2 cell death.
Title: Eukaryotic elongation factor 2 (eEF2) kinase/eEF2 plays protective roles against glucose deprivation-induced cell death in H9c2 cardiomyoblasts.
HSP70 also interacted with C-terminal fragment of eEF2, which could reverse the nucleus translocation and cardiomyocyte apoptosis caused by N-terminal fragment of eEF2. HSP70 draw on advantage and avoid defect of MIR through regulating phosphorylation and nucleus translocation of eEF2
Title: Heat shock protein 70 protects cardiomyocytes through suppressing SUMOylation and nucleus translocation of phosphorylated eukaryotic elongation factor 2 during myocardial ischemia and reperfusion.
The eEF2K promotor in IUGR offspring also lost the protective histone covalent modifications associated with unmethylated CGIs.
Title: Intrauterine growth restriction inhibits expression of eukaryotic elongation factor 2 kinase, a regulator of protein translation.
Using an eEF2K gain-of-function design in vitro as well as eEF2K-KO mice, we show that eEF2K activity regulates the excitation/inhibition balance by downregulating vesicle release at inhibitory synapses and tonic inhibition. This translates into a reduced susceptibility of eEF2K-KO mice for seizures without major behavioral alterations with the exception of impaired contextual and trace fear memory
Title: eEF2K/eEF2 Pathway Controls the Excitation/Inhibition Balance and Susceptibility to Epileptic Seizures.
Eukaryotic elongation factor 2 kinase regulates the development of hypertension through oxidative stress-dependent vascular inflammation.
Title: Eukaryotic elongation factor 2 kinase regulates the development of hypertension through oxidative stress-dependent vascular inflammation.
Expression of betaTRCP1 increases the polyubiquitylation of EEF2K in PC12 cells.
Title: Proteasomal degradation of eukaryotic elongation factor-2 kinase (EF2K) is regulated by cAMP-PKA signaling and the SCFβTRCP ubiquitin E3 ligase.
A Ca(2+)-calmodulin-eEF2K-eEF2 signalling cascade, but not AMPK, contributes to the suppression of skeletal muscle protein synthesis during contractions.
Title: A Ca(2+)-calmodulin-eEF2K-eEF2 signalling cascade, but not AMPK, contributes to the suppression of skeletal muscle protein synthesis during contractions.

Submit: New GeneRIF Correction See all GeneRIFs (11)

General gene information

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Markers

AI102667 (e-PCR)
- UniSTS:250496

RH133994 (e-PCR)
- UniSTS:217276

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables calcium ion binding	IEA Inferred from Electronic Annotation more info
enables calmodulin binding	ISO Inferred from Sequence Orthology more info
enables calmodulin binding	ISS Inferred from Sequence or Structural Similarity more info
enables elongation factor-2 kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables elongation factor-2 kinase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables elongation factor-2 kinase activity	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in cellular response to anoxia	IEP Inferred from Expression Pattern more info	PubMed
involved_in cellular response to brain-derived neurotrophic factor stimulus	IEP Inferred from Expression Pattern more info	PubMed
involved_in cellular response to cAMP	IEP Inferred from Expression Pattern more info	PubMed
involved_in cellular response to calcium ion	IEP Inferred from Expression Pattern more info	PubMed
involved_in cellular response to insulin stimulus	IEP Inferred from Expression Pattern more info	PubMed
involved_in myosin II filament disassembly	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of apoptotic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of dendritic spine morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of endocytosis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of synapse assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of protein autophosphorylation	ISO Inferred from Sequence Orthology more info
involved_in regulation of translation at postsynapse	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of translation at postsynapse	IEP Inferred from Expression Pattern more info	PubMed
involved_in regulation of translation at postsynapse	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to ischemia	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to prolactin	IEP Inferred from Expression Pattern more info	PubMed

Component	Evidence Code	Pubs
located_in dendritic spine	IDA Inferred from Direct Assay more info	PubMed
is_active_in glutamatergic synapse	IDA Inferred from Direct Assay more info	PubMed
is_active_in glutamatergic synapse	IMP Inferred from Mutant Phenotype more info	PubMed
is_active_in postsynaptic density	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: eukaryotic elongation factor 2 kinase

Names: calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase; eEF-2 kinase; eEF-2K; elongation factor 2 kinase; eukaroytic elongation factor 2 kinase

NP_037079.1

EC 2.7.11.20

XP_006230219.1

EC 2.7.11.20

XP_006230220.1

EC 2.7.11.20

XP_038957666.1

EC 2.7.11.20

XP_063137875.1

EC 2.7.11.20

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_012947.3 → NP_037079.1 eukaryotic elongation factor 2 kinase

See identical proteins and their annotated locations for NP_037079.1

Status: VALIDATED

Source sequence(s)

JAXUCZ010000001

UniProtKB/Swiss-Prot

O09089, P70531

UniProtKB/TrEMBL

A6I8S8, Q6P757

Related

ENSRNOP00000022726.3, ENSRNOT00000022726.6

Conserved Domains (3) summary

smart00811
Location:120 → 317

Alpha_kinase; Alpha-kinase family

COG0790
Location:545 → 645

TPR; TPR repeat [Signal transduction mechanisms]

sd00010
Location:502 → 535

SLR; SLR repeat [structural motif]

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086019.1 Reference GRCr8

Range

184824420..184888048

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_039101738.2 → XP_038957666.1 eukaryotic elongation factor 2 kinase isoform X2

UniProtKB/TrEMBL

Q6P757

Conserved Domains (3) summary

COG0790
Location:475 → 575

TPR; TPR repeat [Signal transduction mechanisms]

sd00010
Location:432 → 465

SLR; SLR repeat [structural motif]

cl28917
Location:102 → 247

Alpha_kinase; Alpha kinase family
XM_006230157.5 → XP_006230219.1 eukaryotic elongation factor 2 kinase isoform X1

See identical proteins and their annotated locations for XP_006230219.1

UniProtKB/Swiss-Prot

O09089, P70531

UniProtKB/TrEMBL

A6I8S8, Q6P757

Conserved Domains (3) summary

smart00811
Location:120 → 317

Alpha_kinase; Alpha-kinase family

COG0790
Location:545 → 645

TPR; TPR repeat [Signal transduction mechanisms]

sd00010
Location:502 → 535

SLR; SLR repeat [structural motif]
XM_006230158.5 → XP_006230220.1 eukaryotic elongation factor 2 kinase isoform X1

See identical proteins and their annotated locations for XP_006230220.1

UniProtKB/Swiss-Prot

O09089, P70531

UniProtKB/TrEMBL

A6I8S8, Q6P757

Conserved Domains (3) summary

smart00811
Location:120 → 317

Alpha_kinase; Alpha-kinase family

COG0790
Location:545 → 645

TPR; TPR repeat [Signal transduction mechanisms]

sd00010
Location:502 → 535

SLR; SLR repeat [structural motif]
XM_063281805.1 → XP_063137875.1 eukaryotic elongation factor 2 kinase isoform X1

UniProtKB/Swiss-Prot

O09089, P70531

UniProtKB/TrEMBL

A6I8S8