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    TFS1 Tfs1p [ Saccharomyces cerevisiae S288C ]

    Gene ID: 850875, updated on 17-May-2024

    Summary

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    Gene symbol
    TFS1
    Gene description
    Tfs1p
    Primary source
    FungiDB:YLR178C
    Locus tag
    YLR178C
    See related
    SGD:S000004168; AllianceGenome:SGD:S000004168; VEuPathDB:YLR178C
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Saccharomyces cerevisiae S288C (strain: S288C)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
    Also known as
    DKA1; NSP1
    Summary
    Enables peptidase inhibitor activity and phospholipid binding activity. Involved in regulation of Ras protein signal transduction and regulation of proteolysis. Located in fungal-type vacuole lumen and fungal-type vacuole membrane. Human ortholog(s) of this gene implicated in prostate cancer. Orthologous to several human genes including PEBP1 (phosphatidylethanolamine binding protein 1). [provided by Alliance of Genome Resources, Apr 2022]
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    Genomic context

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    See TFS1 in Genome Data Viewer
    Location:
    chromosome: XII
    Exon count:
    1
    Sequence:
    Chromosome: XII; NC_001144.5 (513162..513821, complement)

    Chromosome XII - NC_001144.5Genomic Context describing neighboring genes Neighboring gene Rfx1p Neighboring gene uncharacterized protein Neighboring gene uncharacterized protein Neighboring gene methionine adenosyltransferase SAM1

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_001144.5

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Specific membrane binding of the carboxypeptidase Y inhibitor I(C), a phosphatidylethanolamine-binding protein family member. Mima J, et al. FEBS J, 2006 Dec. PMID 17076703
    2. A phenotypic study of TFS1 mutants differentially altered in the inhibition of Ira2p or CPY. Gombault A, et al. FEMS Yeast Res, 2009 Sep. PMID 19552705
    3. Biochemical analysis of the yeast proteinase inhibitor (IC) homolog ICh and its comparison with IC. Fukada H, et al. Biosci Biotechnol Biochem, 2007 Feb. PMID 17317927
    4. The multiple site binding of carboxypeptidase Y inhibitor (IC) to the cognate proteinase. Implications for the biological roles of the phosphatidylethanolamine-binding protein. Mima J, et al. J Biol Chem, 2003 Aug 8. PMID 12791700
    5. N-terminal acetyl group is essential for the inhibitory function of carboxypeptidase Y inhibitor (I(C)). Mima J, et al. FEBS Lett, 2002 Dec 4. PMID 12459491

    See all (31) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Tfs1p may act as a bridge between cell signaling and intermediate metabolism in yeast.
      Title: Interaction proteomics suggests a new role for the Tfs1 protein in yeast.
    2. The results obtained demonstrate that the inhibition of Ira2p by Tfs1p is the predominant function under the conditions tested.
      Title: A phenotypic study of TFS1 mutants differentially altered in the inhibition of Ira2p or CPY.
    3. show that the accessibility of the surface pocket of Tfs1p, its N-terminal region and the specific electrostatic properties of a large surface region containing these two elements, play a crucial role in interaction with Ira2
      Title: Molecular basis of the Tfs1/Ira2 interaction: a combined protein engineering and molecular modelling study.
    4. The binding of I(C) to phospholipid membranes was first shown using a liposome-binding assay and by surface plasmon resonance , which revealed that the affinity of this inhibitor was not for phosphatidylethanolamine but for anionic phospholipids.
      Title: Specific membrane binding of the carboxypeptidase Y inhibitor I(C), a phosphatidylethanolamine-binding protein family member.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by SGD

    Function Evidence Code Pubs
    enables peptidase inhibitor activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables peptidase inhibitor activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables phospholipid binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables phospholipid binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables serine-type endopeptidase inhibitor activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in regulation of Ras protein signal transduction IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in regulation of Ras protein signal transduction IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in regulation of Ras protein signal transduction IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    involved_in regulation of proteolysis IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in regulation of proteolysis IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in cytoplasm IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in fungal-type vacuole lumen IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in fungal-type vacuole membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    Tfs1p
    NP_013279.1
    • Inhibitor of carboxypeptidase Y (Prc1p), and Ras GAP (Ira2p); phosphatidylethanolamine-binding protein (PEBP) family member and ortholog of hPEBP1/RKIP, a natural metastasis suppressor; targets to vacuolar membranes during stationary phase; acetylated by NatB N-terminal acetyltransferase; protein abundance increases in response to DNA replication stress

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_001144.5 Reference assembly

      Range
      513162..513821 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001182065.1 → NP_013279.1  TPA: Tfs1p [Saccharomyces cerevisiae S288C]

      See identical proteins and their annotated locations for NP_013279.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      D6VYI2, P14306, P30312
      UniProtKB/TrEMBL
      A0A6A5PSQ5, A7A169, B3RH70, C8ZDF0, N1P1A0
      Conserved Domains (1) summary
      cd00866
      Location:36 → 216
      PEBP_euk; PhosphatidylEthanolamine-Binding Protein (PEBP) domain present in eukaryotes
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P14306.2

    Gene LinkOut

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