ATSPO11-1 Spo11/DNA topoisomerase VI, subunit A protein [Arabidopsis thaliana (thale cress)] - Gene

Summary

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Gene symbol: ATSPO11-1
Gene description: Spo11/DNA topoisomerase VI, subunit A protein
Primary source: Araport:AT3G13170
Locus tag: AT3G13170
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: SPO11-1
Summary: Encodes AtSPO11-1, one of the three Arabidopsis homologues of the archaeal DNA topoisomerase VIA subunit (topo VIA). Required for meiotic recombination. AtSPO11-1 and AtSPO11-2 have overlapping functions (i.e. both required for meiotic recombination) whereas AtSPO11-3 functions in DNA replication. AtSPO11-1 accumulates in foci in early G2. At 1 h post-S phase, no foci are observed, but by 3 h a majority (80%) of meiocytes at this time point contain >50 foci. However, by 5 h, AtSPO11-1 foci are no longer detectable. This suggests that the protein undergoes a rapid cycle of accumulation and disappearance in meiocytes over a period of between 1 and 5 h post-S phase.
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Genomic context

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See ATSPO11-1 in Genome Data Viewer

Location:: chromosome: 3

Exon count:: 15

Sequence:: Chromosome: 3; NC_003074.8 (4231395..4234206, complement)

Chromosome 3 - NC_003074.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003074.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Biochemical characterization of the meiosis-essential yet evolutionarily divergent topoisomerase VIB-like protein MTOPVIB from Arabidopsis thaliana.
Title: Biochemical characterization of the meiosis-essential yet evolutionarily divergent topoisomerase VIB-like protein MTOPVIB from Arabidopsis thaliana.
Heterologous Complementation of SPO11-1 and -2 Depends on the Splicing Pattern.
Title: Heterologous Complementation of SPO11-1 and -2 Depends on the Splicing Pattern.
As transposon mobility in plant genomes is restricted by DNA methylation, we used the met1 DNA methyltransferase mutant to investigate the role of heterochromatin in SPO11-1-oligo distributions. Epigenetic activation of meiotic DSBs in proximity to centromeres and transposons occurred in met1 mutants, coincident with reduced nucleosome occupancy, gain of transcription, and H3K4me3
Title: Nucleosomes and DNA methylation shape meiotic DSB frequency in Arabidopsis thaliana transposons and gene regulatory regions.
Results identified a group of spo11-1 mutants with defects in meiosis that specifically correlate with the defects in DNA binding at an identified DNA binding surface on SPO11-1.
Title: A DNA-binding surface of SPO11-1, an Arabidopsis SPO11 orthologue required for normal meiosis.
Data show that the catalytically active tyrosine residues of SPO11-1 are required for meiotic double-strand break induction in Arabidopsis.
Title: The catalytically active tyrosine residues of both SPO11-1 and SPO11-2 are required for meiotic double-strand break induction in Arabidopsis.
AtSPO11-1 and AtSPO11-2 genetically interact and function in meiotic recombination. [AtSPO11-1]
Title: Arabidopsis SPO11-2 functions with SPO11-1 in meiotic recombination.

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General gene information

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Homology

Orthologs from OrthoDB

NM_001338034.1

Supported by ESTs:

NM_001338035.1

Supported by ESTs:

NM_112156.2

Supported by ESTs:

Gene Ontology Provided by TAIR

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA metabolic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within chiasma assembly	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within homologous chromosome pairing at meiosis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within maintenance of meiotic sister chromatid cohesion	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within meiotic DNA double-strand break formation	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within meiotic DNA double-strand break processing	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within reciprocal meiotic recombination	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in chromosome	IEA Inferred from Electronic Annotation more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	ISM Inferred from Sequence Model more info

General protein information

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Preferred Names: Spo11/DNA topoisomerase VI, subunit A protein

NP_187923.1

ATSPO11-1; CONTAINS InterPro DOMAIN/s: Spo11/DNA topoisomerase VI, subunit A, N-terminal (InterPro:IPR013049), Meiotic recombination, Spo11 (InterPro:IPR013048), Spo11/DNA topoisomerase VI, subunit A (InterPro:IPR002815); BEST Arabidopsis thaliana protein match is: Spo11/DNA topoisomerase VI, subunit A protein (TAIR:AT5G02820.1); Has 908 Blast hits to 908 proteins in 313 species: Archae - 216; Bacteria - 16; Metazoa - 170; Fungi - 122; Plants - 151; Viruses - 0; Other Eukaryotes - 233 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003074.8 Reference assembly

Range

4231395..4234206 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_112156.2 → NP_187923.1 Spo11/DNA topoisomerase VI, subunit A protein [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_187923.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q9C5S3, Q9LK56, Q9M4A2

UniProtKB/TrEMBL

A0A178VGV5, A0A5S9XC69, A0A654FGW4

Conserved Domains (1) summary

COG1697
Location:6 → 357

Spo11; DNA topoisomerase VI, subunit A [Replication, recombination and repair]
NM_001338034.1 → NP_001326966.1 Spo11/DNA topoisomerase VI, subunit A protein [Arabidopsis thaliana]

Status: REVIEWED

UniProtKB/TrEMBL

A0A1I9LQV8
NM_001338035.1 → NP_001326967.1 Spo11/DNA topoisomerase VI, subunit A protein [Arabidopsis thaliana]

Status: REVIEWED

UniProtKB/TrEMBL

A0A1I9LQV8