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    Pola2 polymerase (DNA directed), alpha 2 [ Mus musculus (house mouse) ]

    Gene ID: 18969, updated on 12-May-2024

    Summary

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    Official Symbol
    Pola2provided by MGI
    Official Full Name
    polymerase (DNA directed), alpha 2provided by MGI
    Primary source
    MGI:MGI:99690
    See related
    Ensembl:ENSMUSG00000024833 AllianceGenome:MGI:99690
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Mus musculus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
    Summary
    Predicted to enable DNA-directed DNA polymerase activity. Involved in DNA replication, synthesis of RNA primer. Acts upstream of or within DNA replication and protein import into nucleus. Located in nucleus. Part of alpha DNA polymerase:primase complex. Is expressed in several structures, including alimentary system; brain; genitourinary system; integumental system; and sensory organ. Orthologous to human POLA2 (DNA polymerase alpha 2, accessory subunit). [provided by Alliance of Genome Resources, Apr 2022]
    Expression
    Ubiquitous expression in liver E14.5 (RPKM 23.1), liver E14 (RPKM 19.9) and 28 other tissues See more
    Orthologs
    human all
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    Genomic context

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    See Pola2 in Genome Data Viewer
    Location:
    19 A; 19 4.34 cM
    Exon count:
    21
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCm39 (GCF_000001635.27) 19 NC_000085.7 (5990574..6014266, complement)
    108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 19 NC_000085.6 (5940543..5964238, complement)

    Chromosome 19 - NC_000085.7Genomic Context describing neighboring genes Neighboring gene STARR-positive B cell enhancer ABC_E7628 Neighboring gene predicted gene, 42067 Neighboring gene CDC42 effector protein 2 Neighboring gene STARR-positive B cell enhancer ABC_E8731 Neighboring gene solute carrier family 22 (organic anion transporter), member 20 Neighboring gene predicted gene, 31719 Neighboring gene calpain 1 Neighboring gene predicted gene 10814

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: Mouse ENCODE transcriptome data
    • Description: RNA profiling data sets generated by the Mouse ENCODE project.
    • BioProject: PRJNA66167
    • Publication: PMID 25409824
    • Analysis date: n/a

    Bibliography

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    Related articles in PubMed

    1. Molecular architecture of the mouse DNA polymerase alpha-primase complex. Mizuno T, et al. Mol Cell Biol, 1999 Nov. PMID 10523676, Free PMC Article
    2. Molecular cloning of the cDNAs for the four subunits of mouse DNA polymerase alpha-primase complex and their gene expression during cell proliferation and the cell cycle. Miyazawa H, et al. J Biol Chem, 1993 Apr 15. PMID 8463324
    3. Role of the second-largest subunit of DNA polymerase alpha in the interaction between the catalytic subunit and hyperphosphorylated retinoblastoma protein in late S phase. Takemura M, et al. Biochim Biophys Acta, 2006 Sep. PMID 16935576
    4. The second-largest subunit of the mouse DNA polymerase alpha-primase complex facilitates both production and nuclear translocation of the catalytic subunit of DNA polymerase alpha. Mizuno T, et al. Mol Cell Biol, 1998 Jun. PMID 9584195, Free PMC Article
    5. DNA replication in vitro by recombinant DNA-polymerase-alpha-primase. Stadlbauer F, et al. Eur J Biochem, 1994 Jun 15. PMID 8026492

    See all (30) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. nonphosphorylated p68 inhibited the stimulation of pol-alpha activity by hyperphosphorylated retinoblastoma protein, suggesting that p68 might impede the association of ppRb with p180 [Dna polymerase alpha p68]
      Title: Role of the second-largest subunit of DNA polymerase alpha in the interaction between the catalytic subunit and hyperphosphorylated retinoblastoma protein in late S phase.
    Submit: New GeneRIF Correction

    Variation

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    Alleles

    Alleles of this type are documented at Mouse Genome Informatics  (MGI)
    • Endonuclease-mediated (2) 
    • Targeted (2) 

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Markers

    Homology

    Gene Ontology Provided by MGI

    Function Evidence Code Pubs
    enables DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA-directed DNA polymerase activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    acts_upstream_of_or_within DNA replication IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in DNA replication ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in DNA replication initiation IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in DNA replication initiation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in DNA replication initiation ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in DNA replication, synthesis of primer IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in DNA replication, synthesis of primer ISO
    Inferred from Sequence Orthology
    more info
    		  
    acts_upstream_of_or_within protein import into nucleus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Component Evidence Code Pubs
    part_of alpha DNA polymerase:primase complex IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of alpha DNA polymerase:primase complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of alpha DNA polymerase:primase complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    part_of alpha DNA polymerase:primase complex ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in cytosol ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in nucleoplasm ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in nucleus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    DNA polymerase alpha subunit B
    Names
    DNA polymerase alpha 2, 68 kDa
    DNA polymerase alpha 70 kDa subunit
    NP_001157529.1
    NP_032919.2
    XP_006531744.1
    XP_006531746.1
    XP_006531747.1
    XP_011246909.1
    XP_011246910.1
    XP_011246911.1
    XP_011246912.1
    XP_030106634.1

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001164057.2 → NP_001157529.1  DNA polymerase alpha subunit B isoform 2

      See identical proteins and their annotated locations for NP_001157529.1

      Status: VALIDATED

      Description
      Transcript Variant: This variant (2) uses an alternate splice site in the 3' coding region, compared to variant 1. The resulting isoform (2) has a shorter and distinct C-terminus, compared to isoform 1.
      Source sequence(s)
      AC131692
      Consensus CDS
      CCDS50362.1
      UniProtKB/TrEMBL
      Q3UZI8, Q8C2T6
      Related
      ENSMUSP00000128866.2, ENSMUST00000165143.3
      Conserved Domains (2) summary
      pfam04042
      Location:343 → 549
      DNA_pol_E_B; DNA polymerase alpha/epsilon subunit B
      pfam08418
      Location:20 → 238
      Pol_alpha_B_N; DNA polymerase alpha subunit B N-terminal

    2. NM_008893.4 → NP_032919.2  DNA polymerase alpha subunit B isoform 1

      See identical proteins and their annotated locations for NP_032919.2

      Status: VALIDATED

      Description
      Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).
      Source sequence(s)
      AC131692
      Consensus CDS
      CCDS29485.1
      UniProtKB/Swiss-Prot
      P33611, Q8VDR3
      UniProtKB/TrEMBL
      Q3UZI8
      Related
      ENSMUSP00000025752.8, ENSMUST00000025752.15
      Conserved Domains (2) summary
      pfam04042
      Location:343 → 549
      DNA_pol_E_B; DNA polymerase alpha/epsilon subunit B
      pfam08418
      Location:20 → 238
      Pol_alpha_B_N; DNA polymerase alpha subunit B N-terminal

    RNA

    1. NR_177189.1 RNA Sequence

      Status: VALIDATED

      Source sequence(s)
      AC131692

    2. NR_177190.1 RNA Sequence

      Status: VALIDATED

      Source sequence(s)
      AC131692

    RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCm39 C57BL/6J

    Genomic

    1. NC_000085.7 Reference GRCm39 C57BL/6J

      Range
      5990574..6014266 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_006531681.4 → XP_006531744.1  DNA polymerase alpha subunit B isoform X2

      UniProtKB/TrEMBL
      Q3UZI8
      Conserved Domains (2) summary
      pfam04042
      Location:343 → 592
      DNA_pol_E_B; DNA polymerase alpha/epsilon subunit B
      pfam08418
      Location:21 → 238
      Pol_alpha_B_N; DNA polymerase alpha subunit B N-terminal

    2. XM_011248610.3 → XP_011246912.1  DNA polymerase alpha subunit B isoform X5

      UniProtKB/TrEMBL
      Q3UZI8
      Conserved Domains (2) summary
      pfam04042
      Location:358 → 564
      DNA_pol_E_B; DNA polymerase alpha/epsilon subunit B
      pfam08418
      Location:21 → 253
      Pol_alpha_B_N; DNA polymerase alpha subunit B N-terminal

    3. XM_011248607.3 → XP_011246909.1  DNA polymerase alpha subunit B isoform X1

      UniProtKB/TrEMBL
      Q3UZI8
      Conserved Domains (2) summary
      pfam04042
      Location:358 → 607
      DNA_pol_E_B; DNA polymerase alpha/epsilon subunit B
      pfam08418
      Location:21 → 253
      Pol_alpha_B_N; DNA polymerase alpha subunit B N-terminal

    4. XM_006531683.5 → XP_006531746.1  DNA polymerase alpha subunit B isoform X6

      UniProtKB/TrEMBL
      Q3UZI8
      Conserved Domains (2) summary
      pfam04042
      Location:303 → 552
      DNA_pol_E_B; DNA polymerase alpha/epsilon subunit B
      pfam08418
      Location:15 → 198
      Pol_alpha_B_N; DNA polymerase alpha subunit B N-terminal

    5. XM_011248609.3 → XP_011246911.1  DNA polymerase alpha subunit B isoform X4

      UniProtKB/TrEMBL
      Q3UZI8
      Conserved Domains (2) summary
      pfam04042
      Location:318 → 567
      DNA_pol_E_B; DNA polymerase alpha/epsilon subunit B
      pfam08418
      Location:15 → 213
      Pol_alpha_B_N; DNA polymerase alpha subunit B N-terminal

    6. XM_006531684.5 → XP_006531747.1  DNA polymerase alpha subunit B isoform X10

      Conserved Domains (1) summary
      pfam04042
      Location:40 → 289
      DNA_pol_E_B; DNA polymerase alpha/epsilon subunit B

    7. XM_030250774.2 → XP_030106634.1  DNA polymerase alpha subunit B isoform X7

      UniProtKB/TrEMBL
      Q3UZI8
      Conserved Domains (2) summary
      pfam04042
      Location:358 → 564
      DNA_pol_E_B; DNA polymerase alpha/epsilon subunit B
      pfam08418
      Location:21 → 253
      Pol_alpha_B_N; DNA polymerase alpha subunit B N-terminal

    8. XM_011248608.4 → XP_011246910.1  DNA polymerase alpha subunit B isoform X3

      UniProtKB/TrEMBL
      Q3UZI8
      Conserved Domains (2) summary
      pfam04042
      Location:358 → 607
      DNA_pol_E_B; DNA polymerase alpha/epsilon subunit B
      pfam08418
      Location:21 → 253
      Pol_alpha_B_N; DNA polymerase alpha subunit B N-terminal

    RNA

    1. XR_004940121.1 RNA Sequence

    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P33611.2 GenPept UniProtKB/Swiss-Prot:P33611

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