Pde2a phosphodiesterase 2A [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Pde2aprovided by RGD
Official Full Name: phosphodiesterase 2Aprovided by RGD
Primary source: RGD:620965
See related: Ensembl:ENSRNOG00000019560 AllianceGenome:RGD:620965
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: Pde2; Pde2a2; CGS-PDE
Summary: Enables identical protein binding activity. Involved in several processes, including establishment of endothelial barrier; negative regulation of cAMP-mediated signaling; and response to hydrogen sulfide. Located in several cellular components, including hippocampal mossy fiber; membrane raft; and mitochondrion. Biomarker of cardiomyopathy. Orthologous to human PDE2A (phosphodiesterase 2A). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in Brain (RPKM 258.3), Adrenal (RPKM 218.3) and 8 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 1q32

Exon count:: 32

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	1	NC_086019.1 (165235623..165327466)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	1	NC_051336.1 (155823590..155915434)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	1	NC_005100.4 (166534643..166626158)

Chromosome 1 - NC_086019.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

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Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

CRISPR/Cas9 Knock-Out in Primary Neonatal and Adult Cardiomyocytes Reveals Distinct cAMP Dynamics Regulation by Various PDE2A and PDE3A Isoforms.
Title: CRISPR/Cas9 Knock-Out in Primary Neonatal and Adult Cardiomyocytes Reveals Distinct cAMP Dynamics Regulation by Various PDE2A and PDE3A Isoforms.
PDE2 regulates membrane potential, respiration and permeability transition of rodent subsarcolemmal cardiac mitochondria.
Title: PDE2 regulates membrane potential, respiration and permeability transition of rodent subsarcolemmal cardiac mitochondria.
Using pharmacological and genetic approaches combined with real-time FRET imaging and high resolution microscopy, we demonstrate that in rat cardiac myocytes and other cell types mitochondrial PDE2A2 regulates local cAMP levels and PKA-dependent phosphorylation of Drp1.
Title: PDE2A2 regulates mitochondria morphology and apoptotic cell death via local modulation of cAMP/PKA signalling.
results indicate interventricular differences in PDE2 transcript and activity levels, which may distinctly regulate beta2 adrenoceptor-mediated contractility and cAMP concentrations in the right and left ventricles
Title: PDE2 activity differs in right and left rat ventricular myocardium and differentially regulates β2 adrenoceptor-mediated effects.
Cardiac hypertrophy is inhibited by a local pool of cAMP regulated by PDE2A.
Title: Cardiac Hypertrophy Is Inhibited by a Local Pool of cAMP Regulated by Phosphodiesterase 2.
Pde2 overexpression decreased BNP stimulation of cGMP and BNP responses to the Ca current, intracellular Ca transient, and neurotransmitter release. Pde2 was higher in stellate neurons from prohypertensive rats vs normotensives.
Title: Efficacy of B-Type Natriuretic Peptide Is Coupled to Phosphodiesterase 2A in Cardiac Sympathetic Neurons.
Atrial natriuretic peptide mediates negative regulation of TH gene expression via stimulation of PDE2-dependent cAMP breakdown in PC12 cells.
Title: Phosphodiesterase 2 negatively regulates adenosine-induced transcription of the tyrosine hydroxylase gene in PC12 rat pheochromocytoma cells.
Preferential distribution of PDE2A into axons and terminals of the principal neurons suggests roles in regulation of axonal excitability or transmitter release
Title: The distribution of phosphodiesterase 2A in the rat brain.
the phosphodiesterase 2A isoform localized to mitochondria regulates respiration
Title: A phosphodiesterase 2A isoform localized to mitochondria regulates respiration.
Together, these observations indicate that striatal NO producing interneurons can act via the PDE2A in the medium spiny neurons to regulate the cAMP response to dopamine stimulation.
Title: Nitric oxide signaling via cGMP-stimulated phosphodiesterase in striatal neurons.

Submit: New GeneRIF Correction See all GeneRIFs (17)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

D1Rat171 (e-PCR), detects polymorphism
- UniSTS:118538

MARC_12039-12040:1004039373:1 (e-PCR)
- UniSTS:267315

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables 3',5'-cyclic-AMP phosphodiesterase activity	IBA Inferred from Biological aspect of Ancestor more info
enables 3',5'-cyclic-AMP phosphodiesterase activity	IEA Inferred from Electronic Annotation more info
enables 3',5'-cyclic-AMP phosphodiesterase activity	ISO Inferred from Sequence Orthology more info
enables 3',5'-cyclic-AMP phosphodiesterase activity	ISS Inferred from Sequence or Structural Similarity more info
enables 3',5'-cyclic-GMP phosphodiesterase activity	IBA Inferred from Biological aspect of Ancestor more info
enables 3',5'-cyclic-GMP phosphodiesterase activity	IEA Inferred from Electronic Annotation more info
enables 3',5'-cyclic-GMP phosphodiesterase activity	ISO Inferred from Sequence Orthology more info
enables 3',5'-cyclic-nucleotide phosphodiesterase activity	IEA Inferred from Electronic Annotation more info
enables GAF domain binding	ISO Inferred from Sequence Orthology more info
enables TPR domain binding	IEA Inferred from Electronic Annotation more info
enables TPR domain binding	ISO Inferred from Sequence Orthology more info
enables cAMP binding	IEA Inferred from Electronic Annotation more info
enables cAMP binding	ISO Inferred from Sequence Orthology more info
enables cGMP binding	IEA Inferred from Electronic Annotation more info
enables cGMP binding	ISO Inferred from Sequence Orthology more info
enables cGMP-stimulated cyclic-nucleotide phosphodiesterase activity	IBA Inferred from Biological aspect of Ancestor more info
enables cGMP-stimulated cyclic-nucleotide phosphodiesterase activity	IEA Inferred from Electronic Annotation more info
enables cGMP-stimulated cyclic-nucleotide phosphodiesterase activity	ISO Inferred from Sequence Orthology more info
enables identical protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables magnesium ion binding	IEA Inferred from Electronic Annotation more info
enables magnesium ion binding	ISO Inferred from Sequence Orthology more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables phosphate ion binding	IEA Inferred from Electronic Annotation more info
enables phosphate ion binding	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein homodimerization activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein homodimerization activity	IEA Inferred from Electronic Annotation more info
enables protein homodimerization activity	ISO Inferred from Sequence Orthology more info
enables zinc ion binding	IEA Inferred from Electronic Annotation more info
enables zinc ion binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within aorta development	ISO Inferred from Sequence Orthology more info
involved_in cAMP-mediated signaling	IBA Inferred from Biological aspect of Ancestor more info
involved_in cAMP-mediated signaling	IEA Inferred from Electronic Annotation more info
involved_in cAMP-mediated signaling	ISO Inferred from Sequence Orthology more info
involved_in cGMP catabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in cGMP catabolic process	IEA Inferred from Electronic Annotation more info
involved_in cGMP catabolic process	ISO Inferred from Sequence Orthology more info
involved_in cGMP-mediated signaling	IBA Inferred from Biological aspect of Ancestor more info
involved_in cGMP-mediated signaling	IEA Inferred from Electronic Annotation more info
involved_in cGMP-mediated signaling	ISO Inferred from Sequence Orthology more info
involved_in cGMP-mediated signaling	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within cardiac septum development	ISO Inferred from Sequence Orthology more info
involved_in cellular response to 2,3,7,8-tetrachlorodibenzodioxine	IEA Inferred from Electronic Annotation more info
involved_in cellular response to 2,3,7,8-tetrachlorodibenzodioxine	ISO Inferred from Sequence Orthology more info
involved_in cellular response to cAMP	IEA Inferred from Electronic Annotation more info
involved_in cellular response to cAMP	ISO Inferred from Sequence Orthology more info
involved_in cellular response to cGMP	IEA Inferred from Electronic Annotation more info
involved_in cellular response to cGMP	ISO Inferred from Sequence Orthology more info
involved_in cellular response to cGMP	ISS Inferred from Sequence or Structural Similarity more info
involved_in cellular response to granulocyte macrophage colony-stimulating factor stimulus	ISO Inferred from Sequence Orthology more info
involved_in cellular response to granulocyte macrophage colony-stimulating factor stimulus	ISS Inferred from Sequence or Structural Similarity more info
involved_in cellular response to lipopolysaccharide	ISO Inferred from Sequence Orthology more info
involved_in cellular response to macrophage colony-stimulating factor stimulus	IEA Inferred from Electronic Annotation more info
involved_in cellular response to macrophage colony-stimulating factor stimulus	ISO Inferred from Sequence Orthology more info
involved_in cellular response to macrophage colony-stimulating factor stimulus	ISS Inferred from Sequence or Structural Similarity more info
involved_in cellular response to mechanical stimulus	ISO Inferred from Sequence Orthology more info
involved_in cellular response to mechanical stimulus	ISS Inferred from Sequence or Structural Similarity more info
involved_in cellular response to transforming growth factor beta stimulus	IEA Inferred from Electronic Annotation more info
involved_in cellular response to transforming growth factor beta stimulus	ISO Inferred from Sequence Orthology more info
involved_in cellular response to xenobiotic stimulus	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within coronary vasculature development	ISO Inferred from Sequence Orthology more info
involved_in establishment of endothelial barrier	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within heart development	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within heart valve development	ISO Inferred from Sequence Orthology more info
involved_in intracellular signal transduction	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in intracellular signal transduction	ISO Inferred from Sequence Orthology more info
involved_in monocyte differentiation	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of adenylate cyclase-activating G protein-coupled receptor signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of adenylate cyclase-activating G protein-coupled receptor signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of cAMP-mediated signaling	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of cAMP-mediated signaling	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of cAMP-mediated signaling	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of cGMP-mediated signaling	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of cGMP-mediated signaling	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of oxidative phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of transcription by RNA polymerase II	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of transcription by RNA polymerase II	ISS Inferred from Sequence or Structural Similarity more info
involved_in negative regulation of vascular permeability	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of vascular permeability	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of vascular permeability	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of gene expression	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of gene expression	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of inflammatory response	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of inflammatory response	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of vascular permeability	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of vascular permeability	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of vascular permeability	ISS Inferred from Sequence or Structural Similarity more info
involved_in regulation of cAMP-mediated signaling	IEA Inferred from Electronic Annotation more info
involved_in regulation of cAMP-mediated signaling	ISO Inferred from Sequence Orthology more info
involved_in regulation of cAMP-mediated signaling	ISS Inferred from Sequence or Structural Similarity more info
involved_in regulation of cGMP-mediated signaling	IEA Inferred from Electronic Annotation more info
involved_in regulation of cGMP-mediated signaling	ISO Inferred from Sequence Orthology more info
involved_in regulation of mitochondrion organization	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of mitochondrion organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of mitochondrion organization	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within regulation of nitric oxide mediated signal transduction	ISO Inferred from Sequence Orthology more info
involved_in response to hydrogen sulfide	IEP Inferred from Expression Pattern more info	PubMed
involved_in signal transduction	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within ventricular septum development	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in Golgi apparatus	ISO Inferred from Sequence Orthology more info
located_in Golgi apparatus	ISS Inferred from Sequence or Structural Similarity more info
located_in axon	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in cytosol	ISS Inferred from Sequence or Structural Similarity more info
located_in dendrite	ISO Inferred from Sequence Orthology more info
located_in endoplasmic reticulum	ISO Inferred from Sequence Orthology more info
located_in endoplasmic reticulum	ISS Inferred from Sequence or Structural Similarity more info
located_in hippocampal mossy fiber	IDA Inferred from Direct Assay more info	PubMed
is_active_in mitochondrial inner membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in mitochondrial inner membrane	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrial inner membrane	IEA Inferred from Electronic Annotation more info
located_in mitochondrial inner membrane	ISO Inferred from Sequence Orthology more info
is_active_in mitochondrial matrix	IBA Inferred from Biological aspect of Ancestor more info
located_in mitochondrial matrix	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrial matrix	ISO Inferred from Sequence Orthology more info
is_active_in mitochondrial outer membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in mitochondrial outer membrane	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrial outer membrane	IEA Inferred from Electronic Annotation more info
located_in mitochondrial outer membrane	ISO Inferred from Sequence Orthology more info
located_in mitochondrion	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrion	ISO Inferred from Sequence Orthology more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IEA Inferred from Electronic Annotation more info
located_in nucleus	ISO Inferred from Sequence Orthology more info
located_in nucleus	ISS Inferred from Sequence or Structural Similarity more info
is_active_in perinuclear region of cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in perinuclear region of cytoplasm	IEA Inferred from Electronic Annotation more info
located_in perinuclear region of cytoplasm	ISO Inferred from Sequence Orthology more info
located_in perinuclear region of cytoplasm	ISS Inferred from Sequence or Structural Similarity more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	IEA Inferred from Electronic Annotation more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	ISS Inferred from Sequence or Structural Similarity more info
located_in presynaptic membrane	ISO Inferred from Sequence Orthology more info
located_in presynaptic membrane	ISS Inferred from Sequence or Structural Similarity more info
is_active_in synaptic membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in synaptic membrane	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: cGMP-dependent 3',5'-cyclic phosphodiesterase

Names: cyclic GMP-stimulated phosphodiesterase; phosphodiesterase 2A, cGMP-stimulated

NP_001137319.1

EC 3.1.4.17

NP_001257533.1

EC 3.1.4.17

NP_112341.1

EC 3.1.4.17

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001143847.2 → NP_001137319.1 cGMP-dependent 3',5'-cyclic phosphodiesterase isoform PDE2A3

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (PDE2A3). This variant is assembled from partial rat transcripts. The full-length exon combination is based on the mouse ortholog, NM_001008548.4.

Source sequence(s)

JAXUCZ010000001

UniProtKB/TrEMBL

F8WFW5

Related

ENSRNOP00000026586.4, ENSRNOT00000026586.6

Conserved Domains (2) summary

smart00065
Location:404 → 553

GAF; Domain present in phytochromes and cGMP-specific phosphodiesterases

pfam00233
Location:650 → 883

PDEase_I; 3'5'-cyclic nucleotide phosphodiesterase
NM_001270604.2 → NP_001257533.1 cGMP-dependent 3',5'-cyclic phosphodiesterase isoform PDE2A1

Status: VALIDATED

Description

Transcript Variant: This variant (3) differs in the 5' UTR and has an alternate exon in the 5' UTR, compared to variant 1. These differences cause translation initiation at an alternate AUG and result in an isoform (PDE2A1) that is shorter and has a distinct N-terminus, compared to isoform PDE2A3.

Source sequence(s)

JAXUCZ010000001

UniProtKB/TrEMBL

A0A0G2K876, A0A8I5ZM10

Related

ENSRNOP00000078823.1, ENSRNOT00000120138.1

Conserved Domains (2) summary

smart00065
Location:385 → 534

GAF; Domain present in phytochromes and cGMP-specific phosphodiesterases

pfam00233
Location:631 → 864

PDEase_I; 3'5'-cyclic nucleotide phosphodiesterase
NM_031079.2 → NP_112341.1 cGMP-dependent 3',5'-cyclic phosphodiesterase isoform PDE2A2

See identical proteins and their annotated locations for NP_112341.1

Status: VALIDATED

Description

Transcript Variant: This variant (2) represents use of an alternate promoter and has a distinct 5' UTR and 5' coding region, compared to variant 1. The resulting isoform (2) has a shorter and distinct N-terminus, compared to isoform 1.

Source sequence(s)

JAXUCZ010000001

UniProtKB/Swiss-Prot

Q01062

UniProtKB/TrEMBL

A6I6U9, F8WFW5

Conserved Domains (2) summary

smart00065
Location:397 → 546

GAF; Domain present in phytochromes and cGMP-specific phosphodiesterases

pfam00233
Location:643 → 876

PDEase_I; 3'5'-cyclic nucleotide phosphodiesterase

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086019.1 Reference GRCr8

Range

165235623..165327466

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Suppressed Reference Sequence(s)

The following Reference Sequences have been suppressed. Explain

NR_073045.1: Suppressed sequence

Description

NR_073045.1: This RefSeq was permanently suppressed because currently there is insufficient support for the transcript.