Smurf2 SMAD specific E3 ubiquitin protein ligase 2 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Smurf2provided by RGD
Official Full Name: SMAD specific E3 ubiquitin protein ligase 2provided by RGD
Primary source: RGD:1310067
See related: Ensembl:ENSRNOG00000014623 AllianceGenome:RGD:1310067
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: RGD1310067
Summary: Enables SMAD binding activity and transforming growth factor beta receptor binding activity. Predicted to be involved in several processes, including negative regulation of transmembrane receptor protein serine/threonine kinase signaling pathway; positive regulation of trophoblast cell migration; and ubiquitin-dependent protein catabolic process. Predicted to be located in nuclear speck. Predicted to be part of ubiquitin ligase complex. Predicted to be active in cytoplasm. Orthologous to human SMURF2 (SMAD specific E3 ubiquitin protein ligase 2). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in Lung (RPKM 231.8), Kidney (RPKM 110.1) and 9 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 10q32.1

Exon count:: 20

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	10	NC_086028.1 (92261516..92362185, complement)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	10	NC_051345.1 (91761798..91862485, complement)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	10	NC_005109.4 (95018050..95118107, complement)

Chromosome 10 - NC_086028.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

MiR-568 mitigated cardiomyocytes apoptosis, oxidative stress response and cardiac dysfunction via targeting SMURF2 in heart failure rats.
Title: MiR-568 mitigated cardiomyocytes apoptosis, oxidative stress response and cardiac dysfunction via targeting SMURF2 in heart failure rats.
Bone marrow mesenchymal stem cell-derived exosomes improve renal fibrosis via regulating Smurf 2/Smad 7.
Title: Bone marrow mesenchymal stem cell-derived exosomes improve renal fibrosis via regulating Smurf 2/Smad 7.
The effects of miR-27a-3p-mediated Smurf2 on bleomycin A5-induced pulmonary fibrosis in rats.
Title: The effects of miR-27a-3p-mediated Smurf2 on bleomycin A5-induced pulmonary fibrosis in rats.
MiR-411-3p alleviates Silica-induced pulmonary fibrosis by regulating Smurf2/TGF-beta signaling.
Title: MiR-411-3p alleviates Silica-induced pulmonary fibrosis by regulating Smurf2/TGF-β signaling.
By inhibition of Smurf2 translation, miR-322/503 induces EZH2 expression and activates Akt/GSK3beta pathway, thereby protecting cardiac myocytes from ischemia reperfusion injury.
Title: Inhibition of Smurf2 translation by miR-322/503 protects from ischemia-reperfusion injury by modulating EZH2/Akt/GSK3β signaling.
The effect of smurf2 on the degradation of SnoN by ubiquitination was found to be a key factor in diabetic nephropathy, which was mediated by the TGFbeta1/Smad signaling pathway.
Title: Molecular mechanism of smurf2 in regulating the expression of SnoN in diabetic nephropathy.
These data identify Smurf2 as a novel regulator of connexin43 gap junctions.
Title: Smad ubiquitination regulatory factor-2 controls gap junction intercellular communication by modulating endocytosis and degradation of connexin43.
Smurf2 interacts with Smad7 to suppress TGF-beta-mediated liver fibrosis through the ubiquitin-dependent degradation of TbetaRI during the early period of liver fibrosis.
Title: Overexpression of Smad ubiquitin regulatory factor 2 suppresses transforming growth factor-β mediated liver fibrosis.
Smurf2 siRNA did not influence the effects of TGF-beta(1) on the expression of Smad7/SnoN/Ski in airway remodeling.
Title: Effects of Arkadia on airway remodeling through enhancing TGF-beta signaling in allergic rats.
Inhibition of PI3K or mTOR reduced the level of Rap1B, which acts downstream of Rheb and mTOR. The ubiquitin E3 ligase Smurf2 mediates the restriction of Rap1B by initiating its degradation.
Title: Rheb and mTOR regulate neuronal polarity through Rap1B.

Submit: New GeneRIF Correction See all GeneRIFs (12)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

RH137820 (e-PCR)
- UniSTS:219907

RH137833 (e-PCR)
- UniSTS:219920

AW535161 (e-PCR)
- UniSTS:247192

RH129636 (e-PCR)
- UniSTS:212933

Smurf2 (e-PCR), detects polymorphism
- UniSTS:527558

RH140093 (e-PCR)
- UniSTS:222174

RH129397 (e-PCR)
- UniSTS:212704

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables SMAD binding	IBA Inferred from Biological aspect of Ancestor more info
enables SMAD binding	IPI Inferred from Physical Interaction more info	PubMed
enables SMAD binding	ISO Inferred from Sequence Orthology more info
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables transforming growth factor beta receptor binding	IPI Inferred from Physical Interaction more info	PubMed
enables ubiquitin protein ligase activity	IBA Inferred from Biological aspect of Ancestor more info
enables ubiquitin protein ligase activity	ISO Inferred from Sequence Orthology more info
enables ubiquitin-protein transferase activity	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in negative regulation of BMP signaling pathway	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of transforming growth factor beta receptor signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of trophoblast cell migration	ISO Inferred from Sequence Orthology more info
involved_in proteasome-mediated ubiquitin-dependent protein catabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein ubiquitination	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein ubiquitination	IEA Inferred from Electronic Annotation more info
involved_in ubiquitin-dependent protein catabolic process	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in nuclear speck	ISO Inferred from Sequence Orthology more info
part_of ubiquitin ligase complex	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: E3 ubiquitin-protein ligase SMURF2

NP_001100531.1

EC 2.3.2.26

XP_038942099.1

EC 2.3.2.26

XP_063125283.1

EC 2.3.2.26

XP_063125285.1

EC 2.3.2.26

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001107061.3 → NP_001100531.1 E3 ubiquitin-protein ligase SMURF2

See identical proteins and their annotated locations for NP_001100531.1

Status: PROVISIONAL

Source sequence(s)

JAXUCZ010000010

UniProtKB/TrEMBL

A0A8I5Y0P5, A6HK62, F1M3F2

Related

ENSRNOP00000019781.5, ENSRNOT00000019781.7

Conserved Domains (5) summary

cd08382
Location:13 → 137

C2_Smurf-like; C2 domain present in Smad ubiquitination-related factor (Smurf)-like proteins

smart00456
Location:298 → 330

WW; Domain with 2 conserved Trp (W) residues

COG5104
Location:155 → 236

PRP40; Splicing factor [RNA processing and modification]

cd00078
Location:393 → 745

HECTc; HECT domain; C-terminal catalytic domain of a subclass of Ubiquitin-protein ligase (E3). It binds specific ubiquitin-conjugating enzymes (E2), accepts ubiquitin from E2, transfers ubiquitin to substrate lysine side chains, and transfers additional ...

pfam00397
Location:159 → 188

WW; WW domain

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086028.1 Reference GRCr8

Range

92261516..92362185 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_039086171.2 → XP_038942099.1 E3 ubiquitin-protein ligase SMURF2 isoform X2

UniProtKB/TrEMBL

A0A8I5Y0P5, A0A8I5ZUV2

Related

ENSRNOP00000082927.1

Conserved Domains (5) summary

cd08382
Location:13 → 137

C2_Smurf-like; C2 domain present in Smad ubiquitination-related factor (Smurf)-like proteins

smart00456
Location:252 → 283

WW; Domain with 2 conserved Trp (W) residues

COG5104
Location:155 → 236

PRP40; Splicing factor [RNA processing and modification]

cd00078
Location:340 → 692

HECTc; HECT domain; C-terminal catalytic domain of a subclass of Ubiquitin-protein ligase (E3). It binds specific ubiquitin-conjugating enzymes (E2), accepts ubiquitin from E2, transfers ubiquitin to substrate lysine side chains, and transfers additional ...

pfam00397
Location:159 → 188

WW; WW domain
XM_063269213.1 → XP_063125283.1 E3 ubiquitin-protein ligase SMURF2 isoform X1

UniProtKB/TrEMBL

A0A8I5Y0P5, A0A8I6A1F1
XM_063269215.1 → XP_063125285.1 E3 ubiquitin-protein ligase SMURF2 isoform X3