Rab27a RAB27A, member RAS oncogene family [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Rab27aprovided by RGD
Official Full Name: RAB27A, member RAS oncogene familyprovided by RGD
Primary source: RGD:620918
See related: Ensembl:ENSRNOG00000052499 AllianceGenome:RGD:620918
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: ram
Summary: Enables protein domain specific binding activity. Predicted to be involved in several processes, including complement-dependent cytotoxicity; regulation of vesicle-mediated transport; and secretion by cell. Predicted to act upstream of or within several processes, including leukocyte degranulation; melanocyte differentiation; and pigment granule localization. Located in Weibel-Palade body; apical plasma membrane; and secretory granule membrane. Human ortholog(s) of this gene implicated in Griscelli syndrome type 2 and non-Langerhans-cell histiocytosis. Orthologous to human RAB27A (RAB27A, member RAS oncogene family). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in Thymus (RPKM 208.2), Spleen (RPKM 168.8) and 8 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 8q24

Exon count:: 10

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	8	NC_086026.1 (82663373..82717267)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	8	NC_051343.1 (73782730..73836630)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	8	NC_005107.4 (79722334..79776228)

Chromosome 8 - NC_086026.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Data show that the intracellular M4M5 loop of human alpha1 subunit of Na+,K+-ATPase (ATP1a1) interacts directly with rat rab GTP-binding protein Rab27a.
Title: A role for Na+,K+-ATPase α1 in regulating Rab27a localisation on melanosomes.
These findings indicated that Madd functions as a guanine nucleotide exchange factor for Rab27 in parotid acinar cells and regulates isoproterenol-stimulated amylase release.
Title: MADD/DENN/Rab3GEP functions as a guanine nucleotide exchange factor for Rab27 during granule exocytosis of rat parotid acinar cells.
cyt b(558) depends on clathrin for internalization, and in activated macrophages NADPH oxidase occupies a Rab27A/B-regulated secretory compartment
Title: NADPH oxidase is internalized by clathrin-coated pits and localizes to a Rab27A/B GTPase-regulated secretory compartment in activated macrophages.
Three Rab3/27 effectors, Granuphilin, Noc2, and Rabphilin, in PC12 cells using fluorescence recovery after photobleaching of EGFP-tagged proteins.
Title: The Rab27 effector Rabphilin, unlike Granuphilin and Noc2, rapidly exchanges between secretory granules and cytosol in PC12 cells.
Young secretory granules have a higher capacity for binding Rab3A and Rab27A is functionally important for preferential exocytosis from these granules.
Title: Differential dynamics of Rab3A and Rab27A on secretory granules.
Our results suggest that the Noc2/Rab27 complex is an important constituent of the early stages of IPR-stimulated amylase release.
Title: Functional involvement of Noc2, a Rab27 effector, in rat parotid acinar cells.
Rab27A interacts with Rabphilin and Noc2 which are recruited to dense-core vesicles in rat cells
Title: Rabphilin and Noc2 are recruited to dense-core vesicles through specific interaction with Rab27A in PC12 cells.
rab27a is a direct partner of munc13-4. The complex of the two proteins is an essential regulator of secretory granule fusion with the plasma membrane in hematopietic cells. Mutations in either of the two genes prevent formation of this compex.
Title: Munc13-4 is an effector of rab27a and controls secretion of lysosomes in hematopoietic cells.
Results demonstrate that Rab27A plays a crucial role in antigen-induced histamine release from RBL-2H3 cells.
Title: Involvement of Rab27 in antigen-induced histamine release from rat basophilic leukemia 2H3 cells.

Submit: New GeneRIF Correction

General gene information

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Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables G protein activity	IEA Inferred from Electronic Annotation more info
enables GDP binding	ISO Inferred from Sequence Orthology more info
enables GDP binding	ISS Inferred from Sequence or Structural Similarity more info
enables GTP binding	IBA Inferred from Biological aspect of Ancestor more info
enables GTP binding	ISO Inferred from Sequence Orthology more info
enables GTP binding	ISS Inferred from Sequence or Structural Similarity more info
enables GTPase activity	IBA Inferred from Biological aspect of Ancestor more info
enables GTPase activity	ISO Inferred from Sequence Orthology more info
enables GTPase activity	ISS Inferred from Sequence or Structural Similarity more info
enables myosin V binding	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein domain specific binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in antigen processing and presentation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within blood coagulation	ISO Inferred from Sequence Orthology more info
involved_in complement-dependent cytotoxicity	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within cytotoxic T cell degranulation	ISO Inferred from Sequence Orthology more info
involved_in exocytosis	IBA Inferred from Biological aspect of Ancestor more info
involved_in exocytosis	ISO Inferred from Sequence Orthology more info
involved_in exocytosis	TAS Traceable Author Statement more info	PubMed
involved_in exosomal secretion	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within melanocyte differentiation	ISO Inferred from Sequence Orthology more info
involved_in melanosome localization	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within melanosome transport	ISO Inferred from Sequence Orthology more info
involved_in multivesicular body organization	ISO Inferred from Sequence Orthology more info
involved_in multivesicular body sorting pathway	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within natural killer cell degranulation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within pigment granule localization	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within pigment granule transport	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within pigmentation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of constitutive secretory pathway	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of exocytosis	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of exocytosis	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of gene expression	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of phagocytosis	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of reactive oxygen species biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of regulated secretory pathway	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within protein secretion	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within vesicle-mediated transport	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
is_active_in Golgi apparatus	IBA Inferred from Biological aspect of Ancestor more info
located_in Golgi apparatus	ISO Inferred from Sequence Orthology more info
located_in Weibel-Palade body	IDA Inferred from Direct Assay more info	PubMed
is_active_in apical plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in apical plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in dendrite	ISO Inferred from Sequence Orthology more info
is_active_in exocytic vesicle	IBA Inferred from Biological aspect of Ancestor more info
located_in exocytic vesicle	ISO Inferred from Sequence Orthology more info
located_in late endosome	ISO Inferred from Sequence Orthology more info
located_in late endosome	ISS Inferred from Sequence or Structural Similarity more info
located_in lysosome	ISO Inferred from Sequence Orthology more info
is_active_in melanosome	IBA Inferred from Biological aspect of Ancestor more info
located_in melanosome	IDA Inferred from Direct Assay more info	PubMed
located_in melanosome	ISO Inferred from Sequence Orthology more info
located_in multivesicular body membrane	ISO Inferred from Sequence Orthology more info
located_in photoreceptor outer segment	ISO Inferred from Sequence Orthology more info
is_active_in secretory granule	IBA Inferred from Biological aspect of Ancestor more info
located_in secretory granule	IDA Inferred from Direct Assay more info	PubMed
located_in secretory granule	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: ras-related protein Rab-27A

Names: GTP-binding protein Ram; low Mr GTP-binding protein; rab-27; ram p25

NP_059013.1

EC 3.6.5.2

XP_038937932.1

EC 3.6.5.2

XP_063122103.1

EC 3.6.5.2

XP_063122104.1

EC 3.6.5.2

XP_063122105.1

EC 3.6.5.2

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_017317.2 → NP_059013.1 ras-related protein Rab-27A

See identical proteins and their annotated locations for NP_059013.1

Status: PROVISIONAL

Source sequence(s)

BC098667

UniProtKB/Swiss-Prot

P23640, Q4KMA7

UniProtKB/TrEMBL

A6KEK7

Related

ENSRNOP00000068946.1, ENSRNOT00000083957.2

Conserved Domains (1) summary

cd04127
Location:6 → 185

Rab27A; Rab GTPase family 27a (Rab27a)

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086026.1 Reference GRCr8

Range

82663373..82717267

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_063266033.1 → XP_063122103.1 ras-related protein Rab-27A isoform X1

UniProtKB/Swiss-Prot

P23640, Q4KMA7

UniProtKB/TrEMBL

A6KEK7
XM_039082004.2 → XP_038937932.1 ras-related protein Rab-27A isoform X1

UniProtKB/Swiss-Prot

P23640, Q4KMA7

UniProtKB/TrEMBL

A6KEK7

Conserved Domains (1) summary

cd04127
Location:6 → 185

Rab27A; Rab GTPase family 27a (Rab27a)
XM_063266034.1 → XP_063122104.1 ras-related protein Rab-27A isoform X1

UniProtKB/Swiss-Prot

P23640, Q4KMA7

UniProtKB/TrEMBL

A6KEK7
XM_063266035.1 → XP_063122105.1 ras-related protein Rab-27A isoform X1

UniProtKB/Swiss-Prot

P23640, Q4KMA7

UniProtKB/TrEMBL

A6KEK7