Alox12 arachidonate 12-lipoxygenase, 12S type [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Alox12provided by RGD
Official Full Name: arachidonate 12-lipoxygenase, 12S typeprovided by RGD
Primary source: RGD:1311159
See related: Ensembl:ENSRNOG00000027037 AllianceGenome:RGD:1311159
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: 12-LO; 12S-LOX
Summary: Predicted to enable arachidonate 12(S)-lipoxygenase activity; arachidonate 15-lipoxygenase activity; and linoleate 13S-lipoxygenase activity. Involved in several processes, including fatty acid metabolic process; positive regulation of macromolecule metabolic process; and regulation of apoptotic process. Located in cytosol and sarcolemma. Biomarker of congestive heart failure; hypertension; and obesity. Orthologous to human ALOX12 (arachidonate 12-lipoxygenase, 12S type). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in Spleen (RPKM 58.3), Uterus (RPKM 42.3) and 8 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 10q24

Exon count:: 15

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	10	NC_086028.1 (55456923..55469239, complement)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	10	NC_051345.1 (54958263..54970542, complement)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	10	NC_005109.4 (56851734..56864049, complement)

Chromosome 10 - NC_086028.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Angiotensin II type 1 receptor blocker potentially ameliorates diabetic nephropathy via the up-regulation of glomerular nephrin and P-cadherin expression through the inhibition of 12-LO activation in the glomeruli of rats with type 2 diabetes.
Title: Effect of angiotensin II type 1 receptor blocker on 12-lipoxygenase activity and slit diaphragm protein expression in type 2 diabetic rat glomeruli.
The aim of the present study was to examine the impacts and mechanisms of 12lipoxygenase (12LO) and its metabolites on the acetylation and methylation of histone3lysine (H3K) in the p21 gene. associated epigenetic changes, including increased histone H3K9Ac and H3K4Me1, and reduced H3K9M.e3 may involved in 12 (S)hydroxyeicosatetraenoic acid mediated p21 gene regulation.
Title: Role of histone modification in 12‑lipoxygenase‑associated p21 gene regulation.
Hypoxic preconditioning reduces heart susceptibly to ischemia-reperfusion injuryvia ALOX12/TRPV1/PKC pathway.
Title: Hypoxic preconditioning protects rat hearts against ischemia-reperfusion injury via the arachidonate12-lipoxygenase/transient receptor potential vanilloid 1 pathway.
Diabetic proteinuria is mediated by the activation of 12-LO pathway that is partially attributed to the decreased glomerular P-cadherin expression.
Title: Role of 12-lipoxygenase in decreasing P-cadherin and increasing angiotensin II type 1 receptor expression according to glomerular size in type 2 diabetic rats.
Role of Src tyrosine kinase in the atherogenic effects of the 12/15-lipoxygenase pathway in vascular smooth muscle cells.
Title: Role of Src tyrosine kinase in the atherogenic effects of the 12/15-lipoxygenase pathway in vascular smooth muscle cells.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables arachidonate 12(S)-lipoxygenase activity	IBA Inferred from Biological aspect of Ancestor more info
enables arachidonate 12(S)-lipoxygenase activity	ISO Inferred from Sequence Orthology more info
enables arachidonate 12(S)-lipoxygenase activity	ISS Inferred from Sequence or Structural Similarity more info
enables arachidonate 15-lipoxygenase activity	IBA Inferred from Biological aspect of Ancestor more info
enables arachidonate 15-lipoxygenase activity	ISO Inferred from Sequence Orthology more info
enables arachidonate 15-lipoxygenase activity	ISS Inferred from Sequence or Structural Similarity more info
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables iron ion binding	IEA Inferred from Electronic Annotation more info
enables linoleate 13S-lipoxygenase activity	IBA Inferred from Biological aspect of Ancestor more info
enables linoleate 13S-lipoxygenase activity	ISO Inferred from Sequence Orthology more info
enables linoleate 13S-lipoxygenase activity	ISS Inferred from Sequence or Structural Similarity more info
enables oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in arachidonic acid metabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in arachidonic acid metabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in arachidonic acid metabolic process	ISO Inferred from Sequence Orthology more info
involved_in cellular response to lipid	IEP Inferred from Expression Pattern more info	PubMed
involved_in establishment of skin barrier	ISO Inferred from Sequence Orthology more info
involved_in establishment of skin barrier	ISS Inferred from Sequence or Structural Similarity more info
involved_in fatty acid oxidation	ISO Inferred from Sequence Orthology more info
involved_in fatty acid oxidation	ISS Inferred from Sequence or Structural Similarity more info
involved_in hepoxilin biosynthetic process	IDA Inferred from Direct Assay more info	PubMed
NOT involved_in leukotriene A4 metabolic process	ISO Inferred from Sequence Orthology more info
involved_in leukotriene A4 metabolic process	ISO Inferred from Sequence Orthology more info
involved_in leukotriene A4 metabolic process	ISS Inferred from Sequence or Structural Similarity more info
NOT involved_in linoleic acid metabolic process	ISO Inferred from Sequence Orthology more info
involved_in linoleic acid metabolic process	ISO Inferred from Sequence Orthology more info
involved_in linoleic acid metabolic process	ISS Inferred from Sequence or Structural Similarity more info
involved_in lipid metabolic process	ISO Inferred from Sequence Orthology more info
involved_in lipid metabolic process	ISS Inferred from Sequence or Structural Similarity more info
involved_in lipid oxidation	IBA Inferred from Biological aspect of Ancestor more info
involved_in lipid oxidation	IEA Inferred from Electronic Annotation more info
involved_in lipoxin A4 biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in lipoxin A4 biosynthetic process	ISS Inferred from Sequence or Structural Similarity more info
involved_in lipoxin B4 biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in lipoxin B4 biosynthetic process	ISS Inferred from Sequence or Structural Similarity more info
involved_in lipoxygenase pathway	IBA Inferred from Biological aspect of Ancestor more info
involved_in lipoxygenase pathway	IDA Inferred from Direct Assay more info	PubMed
involved_in lipoxygenase pathway	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of apoptotic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of muscle cell apoptotic process	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of muscle cell apoptotic process	ISS Inferred from Sequence or Structural Similarity more info
involved_in negative regulation of platelet aggregation	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of platelet aggregation	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of apoptotic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of blood vessel endothelial cell migration	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of endothelial tube morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of gene expression	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of mitochondrial depolarization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of reactive oxygen species biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of smooth muscle cell proliferation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in unsaturated fatty acid metabolic process	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	ISS Inferred from Sequence or Structural Similarity more info
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in membrane	ISO Inferred from Sequence Orthology more info
located_in membrane	ISS Inferred from Sequence or Structural Similarity more info
is_active_in sarcolemma	IBA Inferred from Biological aspect of Ancestor more info
located_in sarcolemma	IDA Inferred from Direct Assay more info	PubMed
located_in sarcolemma	ISO Inferred from Sequence Orthology more info
located_in sarcolemma	ISS Inferred from Sequence or Structural Similarity more info

General protein information

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Preferred Names: polyunsaturated fatty acid lipoxygenase ALOX12

Names: 12S-lipoxygenase; arachidonate (12S)-lipoxygenase; arachidonate (15S)-lipoxygenase; arachidonate 15-lipoxygenase,15S-type; linoleate 13S-lipoxygenase; lipoxin synthase 12-LO; platelet 12-LOX; platelet-type lipoxygenase 12

NP_001099268.2

EC 1.13.11.31

EC 1.13.11.33

XP_038941407.1

EC 1.13.11.31

EC 1.13.11.33

XP_038941408.1

EC 1.13.11.31

EC 1.13.11.33

XP_063124729.1

EC 1.13.11.31

EC 1.13.11.33

XP_063124730.1

EC 1.13.11.31

EC 1.13.11.33

XP_063124731.1

EC 1.13.11.31

EC 1.13.11.33

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001105798.2 → NP_001099268.2 polyunsaturated fatty acid lipoxygenase ALOX12

Status: PROVISIONAL

Source sequence(s)

JAXUCZ010000010

UniProtKB/Swiss-Prot

F1LQ70

UniProtKB/TrEMBL

A6HG42

Related

ENSRNOP00000034009.4, ENSRNOT00000033098.6

Conserved Domains (2) summary

pfam00305
Location:221 → 654

Lipoxygenase

cl00011
Location:2 → 111

PLAT; PLAT (Polycystin-1, Lipoxygenase, Alpha-Toxin) domain or LH2 (Lipoxygenase homology 2) domain. It consists of an eight stranded beta-barrel. The domain can be found in various domain architectures, in case of lipoxygenases, alpha toxin, lipases and ...

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086028.1 Reference GRCr8

Range

55456923..55469239 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_063268659.1 → XP_063124729.1 polyunsaturated fatty acid lipoxygenase ALOX12 isoform X1

UniProtKB/TrEMBL

A6HG42
XM_039085479.2 → XP_038941407.1 polyunsaturated fatty acid lipoxygenase ALOX12 isoform X2

Conserved Domains (1) summary

pfam00305
Location:58 → 491

Lipoxygenase
XM_039085480.2 → XP_038941408.1 polyunsaturated fatty acid lipoxygenase ALOX12 isoform X3

Conserved Domains (1) summary

PLN02305
Location:19 → 425

PLN02305; lipoxygenase
XM_063268660.1 → XP_063124730.1 polyunsaturated fatty acid lipoxygenase ALOX12 isoform X4

UniProtKB/TrEMBL

A6HG43
XM_063268661.1 → XP_063124731.1 polyunsaturated fatty acid lipoxygenase ALOX12 isoform X5

UniProtKB/TrEMBL

A6HG43