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    MED8 Med8p [ Saccharomyces cerevisiae S288C ]

    Gene ID: 852492, updated on 17-May-2024

    Summary

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    Gene symbol
    MED8
    Gene description
    Med8p
    Primary source
    FungiDB:YBR193C
    Locus tag
    YBR193C
    See related
    SGD:S000000397; AllianceGenome:SGD:S000000397; VEuPathDB:YBR193C
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Saccharomyces cerevisiae S288C (strain: S288C)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
    Summary
    Enables several functions, including RNA polymerase II cis-regulatory region sequence-specific DNA binding activity; TBP-class protein binding activity; and transcription corepressor activity. Involved in negative regulation of transcription by RNA polymerase II and positive regulation of transcription by RNA polymerase II. Part of core mediator complex. [provided by Alliance of Genome Resources, Apr 2022]
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    Genomic context

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    See MED8 in Genome Data Viewer
    Location:
    chromosome: II
    Exon count:
    1
    Sequence:
    Chromosome: II; NC_001134.8 (609082..609753, complement)

    Chromosome II - NC_001134.8Genomic Context describing neighboring genes Neighboring gene ribosomal 60S subunit protein L21A Neighboring gene Rim2p Neighboring gene Aim4p Neighboring gene Msi1p

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_001134.8

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Analysis by atomic force microscopy of Med8 binding to cis-acting regulatory elements of the SUC2 and HXK2 genes of saccharomyces cerevisiae. Moreno-Herrero F, et al. FEBS Lett, 1999 Oct 15. PMID 10526178
    2. Med8, a subunit of the mediator CTD complex of RNA polymerase II, directly binds to regulatory elements of SUC2 and HXK2 genes. Chaves RS, et al. Biochem Biophys Res Commun, 1999 Jan 19. PMID 9918841
    3. Mediator factor Med8p interacts with the hexokinase 2: implication in the glucose signalling pathway of Saccharomyces cerevisiae. de la Cera T, et al. J Mol Biol, 2002 Jun 7. PMID 12054864
    4. Med8, Med18, and Med20 subunits of the Mediator head domain are interdependent upon each other for folding and complex formation. Adler D, et al. Proc Natl Acad Sci U S A, 2009 Dec 8. PMID 19934057, Free PMC Article
    5. Rgt1, a glucose sensing transcription factor, is required for transcriptional repression of the HXK2 gene in Saccharomyces cerevisiae. Palomino A, et al. Biochem J, 2005 Jun 1. PMID 15705057, Free PMC Article

    See all (85) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Deletion of med8C, med18, or med20 causes similar changes in the yeast transcriptome.
      Title: Structure-system correlation identifies a gene regulatory Mediator submodule.
    2. We identified Rgt1 as a transcription factor that, together with the Med8 protein, is essential for repression of the HXK2 gene in the absence of glucose.
      Title: Rgt1, a glucose sensing transcription factor, is required for transcriptional repression of the HXK2 gene in Saccharomyces cerevisiae.
    3. Our results and published data support a positive role of the Med8/18/20 subcomplex in initiation-complex formation and suggest that the Mediator head contains a multipartite TBP-binding site that can be modulated by transcriptional activators.
      Title: Structure and TBP binding of the Mediator head subcomplex Med8-Med18-Med20.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by SGD

    Function Evidence Code Pubs
    enables DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables RNA polymerase II cis-regulatory region sequence-specific DNA binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables RNA polymerase II cis-regulatory region sequence-specific DNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables TBP-class protein binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables protein-macromolecule adaptor activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables transcription coregulator activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables transcription coregulator activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables transcription corepressor activity IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in negative regulation of transcription by RNA polymerase II IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in positive regulation of transcription by RNA polymerase II IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in regulation of transcription by RNA polymerase II IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in regulation of transcription by RNA polymerase II IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    part_of core mediator complex IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of core mediator complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of mediator complex IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of mediator complex IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in nucleus IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    Med8p
    NP_009752.3
    • Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_001134.8 Reference assembly

      Range
      609082..609753 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001178541.3 → NP_009752.3  TPA: Med8p [Saccharomyces cerevisiae S288C]

      See identical proteins and their annotated locations for NP_009752.3

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      D6VQI7, P38304
      UniProtKB/TrEMBL
      A0A6A5Q217, A6ZLD3, C7GKF2, D3UET7, G2W9J4, N1P9K0
      Conserved Domains (1) summary
      pfam10232
      Location:32 → 127
      Med8; Mediator of RNA polymerase II transcription complex subunit 8
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    5OQM_b.1
    P38304.3

    Gene LinkOut

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