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    tktB transketolase 2 [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 945865, updated on 6-Jun-2024

    Summary

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    Gene symbol
    tktB
    Gene description
    transketolase 2
    Primary source
    ASAP:ABE-0008117
    Locus tag
    b2465
    See related
    ECOCYC:EG12100
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK2460
    Summary
    Consistently co-purifies with the degradosome. [More information is available at EcoGene: EG12100]. TktB is responsible for the minor transketolase activity in Escherichia coli, and TktA is responsible for the major transketolase activity . [More information is available at EcoCyc: EG12100].
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    Genomic context

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    Sequence:
    NC_000913.3 (2579636..2581639)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene malate dehydrogenase (oxaloacetate-decarboxylating) (NADP(+)) Neighboring gene transaldolase A Neighboring gene DUF1176 domain-containing protein YpfG Neighboring gene GDP-mannose hydrolase

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Robustness analysis of the Escherichia coli metabolic network. Edwards JS, et al. Biotechnol Prog, 2000 Nov-Dec. PMID 11101318
    2. Enzyme-catalysed carbon-carbon bond formation: large-scale production of Escherichia coli transketolase. Hobbs GR, et al. J Biotechnol, 1996 Feb 28. PMID 9147449
    3. In situ product removal from E. coli transketolase-catalyzed biotransformations. Chauhan RP, et al. Ann N Y Acad Sci, 1996 Oct 12. PMID 8958111
    4. Production and modification of E. coli transketolase for large-scale biocatalysis. French C, et al. Ann N Y Acad Sci, 1996 Oct 12. PMID 8958067
    5. Transketolase A of Escherichia coli K12. Purification and properties of the enzyme from recombinant strains. Sprenger GA, et al. Eur J Biochem, 1995 Jun 1. PMID 7607225

    See all (23) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. tktB is a transcriptional target of ppGpp; activation of tktB transcription by ppGpp occurs both through the modulation of RpoS levels and independent of RpoS.
      Title: Synthetic growth phenotypes of Escherichia coli lacking ppGpp and transketolase A (tktA) are due to ppGpp-mediated transcriptional regulation of tktB.
    2. stationary growth-dependent expression mediated by RpoS action
      Title: RpoS-mediated growth-dependent expression of the Escherichia coli tkt genes encoding transketolases isoenzymes.
    Submit: New GeneRIF Correction

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables transferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables transketolase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables transketolase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables transketolase activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables transketolase or transaldolase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in pentose-phosphate shunt IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    Component Evidence Code Pubs
    is_active_in cytosol IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    transketolase 2
    NP_416960.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      2579636..2581639
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_416960.1 transketolase 2 [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_416960.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A2W6PC01, A0A3Z6G4Q3, A0A8T6BJN4
      Conserved Domains (1) summary
      PRK12753
      Location:1 → 662
      PRK12753; transketolase; Reviewed
    Nucleotide Protein
    Heading Accession and Version

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    Other Literature Sources
    Molecular Biology Databases
    Research Materials