Slc1a3 solute carrier family 1 member 3 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Slc1a3provided by RGD
Official Full Name: solute carrier family 1 member 3provided by RGD
Primary source: RGD:3698
See related: Ensembl:ENSRNOG00000016163 AllianceGenome:RGD:3698
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: EAAT1; GLAST; GluT-1; GLAST-1
Summary: Enables high-affinity glutamate transmembrane transporter activity. Involved in L-aspartate import across plasma membrane; L-glutamate import across plasma membrane; and malate-aspartate shuttle. Acts upstream of or within L-glutamate transmembrane transport and response to inorganic substance. Located in several cellular components, including basolateral plasma membrane; dendritic spine; and neuronal cell body. Is integral component of plasma membrane. Part of membrane protein complex. Human ortholog(s) of this gene implicated in episodic ataxia type 6. Orthologous to human SLC1A3 (solute carrier family 1 member 3). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in Brain (RPKM 1122.4), Adrenal (RPKM 101.1) and 3 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 2q16

Exon count:: 10

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	2	NC_086020.1 (59482707..59557808, complement)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	2	NC_051337.1 (57755495..57830605, complement)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	2	NC_005101.4 (57860881..57935363, complement)

Chromosome 2 - NC_086020.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

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Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

EphA4/ephrinA3 reverse signaling mediated downregulation of glutamate transporter GLAST in Muller cells in an experimental glaucoma model.
Title: EphA4/ephrinA3 reverse signaling mediated downregulation of glutamate transporter GLAST in Müller cells in an experimental glaucoma model.
Glutamate-aspartate transporter 1 attenuates oxygen-glucose deprivation-induced injury by promoting glutamate metabolism in primary cortical neurons.
Title: Glutamate-aspartate transporter 1 attenuates oxygen-glucose deprivation-induced injury by promoting glutamate metabolism in primary cortical neurons.
This study showed an increased expression of glial (GLAST and GLT-1) after paraoxon exposure.
Title: Alterations in mRNA and protein expression of glutamate transporters in rat hippocampus after paraoxon exposure.
EAAT1 is regulated by CaMKII signaling and is a critical driver of constitutive glutamate uptake by EAAT1.
Title: Constitutive regulation of the glutamate/aspartate transporter EAAT1 by Calcium-Calmodulin-Dependent Protein Kinase II.
Astrocyte expression of GLAST/GFAP was reduced via JAK1/JAK2/STAT3 signaling pathway after exposure to sevoflurane.
Title: Sevoflurane Inhibits Glutamate-Aspartate Transporter and Glial Fibrillary Acidic Protein Expression in Hippocampal Astrocytes of Neonatal Rats Through the Janus Kinase/Signal Transducer and Activator of Transcription (JAK/STAT) Pathway.
Spinal glial glutamate transporters GLAST and GLT-1 have protective function by reducing and preventing selectively non-evoked pain behavior after incision. This is a result of increased GLAST expression regulated by the MAPK p38 after incision
Title: Activation of glial glutamate transporter via MAPK p38 prevents enhanced and long-lasting non-evoked resting pain after surgical incision in rats.
Results suggest that, via astrocytes, IL-17A could promote glutamate excitotoxicity in multiple terms: by decreasing the astrocyte ability to uptake extracellular glutamate, due to a reduced gene expression of glutamate transporters (GLAST and GLT-1); by decreasing intracellular glutamate conversion into non-toxic glutamine; by potentiating glutamate exocytosis in a Ca2+ dependent manner
Title: IL-17 signalling in astrocytes promotes glutamate excitotoxicity: Indications for the link between inflammatory and neurodegenerative events in multiple sclerosis.
Chronic postnatal stress led to increased Glast expression in hippocampus and frontal cortex.
Title: Chronic postnatal stress induces voluntary alcohol intake and modifies glutamate transporters in adolescent rats.
EAAT1 most likely mediates Na(+)-dependent high-affinity L-Glutamate (L-GLU) transport at the inner blood retinal barrier (BRB) and appears to take part in L-Glu elimination from the retina across the inner BRB
Title: Excitatory Amino Acid Transporter 1-Mediated L-Glutamate Transport at the Inner Blood-Retinal Barrier: Possible Role in L-Glutamate Elimination from the Retina.
findings suggest that NF-kappaB is a critical positive regulator of EAAT1, mediating the stimulatory effects of EGF, whereas YY1 is a negative regulator of EAAT1 with HDACs as co-repressors
Title: Transcriptional Regulation of the Astrocytic Excitatory Amino Acid Transporter 1 (EAAT1) via NF-κB and Yin Yang 1 (YY1).

Submit: New GeneRIF Correction See all GeneRIFs (51)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

RH143474 (e-PCR)
- UniSTS:232721

RH139535 (e-PCR)
- UniSTS:221621

GDB:335409 (e-PCR)
- UniSTS:156598

AU049927 (e-PCR)
- UniSTS:146369

Slc1a3 (e-PCR), detects polymorphism
- UniSTS:547015

RH140370 (e-PCR)
- UniSTS:222446

AI504299 (e-PCR)
- UniSTS:180734

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables L-glutamate transmembrane transporter activity	IBA Inferred from Biological aspect of Ancestor more info
enables L-glutamate transmembrane transporter activity	IDA Inferred from Direct Assay more info	PubMed
enables L-glutamate transmembrane transporter activity	ISO Inferred from Sequence Orthology more info
enables L-glutamate transmembrane transporter activity	TAS Traceable Author Statement more info	PubMed
enables acidic amino acid transmembrane transporter activity	ISO Inferred from Sequence Orthology more info
enables amino acid binding	ISO Inferred from Sequence Orthology more info
enables glutamate binding	IEA Inferred from Electronic Annotation more info
enables glutamate binding	ISO Inferred from Sequence Orthology more info
enables glutamate:sodium symporter activity	IBA Inferred from Biological aspect of Ancestor more info
enables glutamate:sodium symporter activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables glutamate:sodium symporter activity	ISO Inferred from Sequence Orthology more info
enables glutamate:sodium symporter activity	ISS Inferred from Sequence or Structural Similarity more info
enables high-affinity L-glutamate transmembrane transporter activity	IEA Inferred from Electronic Annotation more info
enables high-affinity L-glutamate transmembrane transporter activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables high-affinity L-glutamate transmembrane transporter activity	ISO Inferred from Sequence Orthology more info
enables high-affinity L-glutamate transmembrane transporter activity	ISS Inferred from Sequence or Structural Similarity more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables neutral L-amino acid transmembrane transporter activity	IBA Inferred from Biological aspect of Ancestor more info

Process	Evidence Code	Pubs
involved_in D-aspartate import across plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
involved_in D-aspartate import across plasma membrane	IEA Inferred from Electronic Annotation more info
involved_in D-aspartate import across plasma membrane	ISO Inferred from Sequence Orthology more info
involved_in D-aspartate import across plasma membrane	ISS Inferred from Sequence or Structural Similarity more info
involved_in L-aspartate import across plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
involved_in L-aspartate import across plasma membrane	IEA Inferred from Electronic Annotation more info
involved_in L-aspartate import across plasma membrane	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in L-aspartate import across plasma membrane	ISO Inferred from Sequence Orthology more info
involved_in L-aspartate import across plasma membrane	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within L-glutamate import	ISO Inferred from Sequence Orthology more info
involved_in L-glutamate import	ISO Inferred from Sequence Orthology more info
involved_in L-glutamate import across plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
involved_in L-glutamate import across plasma membrane	IEA Inferred from Electronic Annotation more info
involved_in L-glutamate import across plasma membrane	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within L-glutamate import across plasma membrane	ISO Inferred from Sequence Orthology more info
involved_in L-glutamate import across plasma membrane	ISO Inferred from Sequence Orthology more info
involved_in L-glutamate import across plasma membrane	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within L-glutamate transmembrane transport	IDA Inferred from Direct Assay more info	PubMed
involved_in L-glutamate transmembrane transport	IDA Inferred from Direct Assay more info	PubMed
involved_in L-glutamate transmembrane transport	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within L-glutamate transmembrane transport	ISO Inferred from Sequence Orthology more info
involved_in L-glutamate transmembrane transport	ISO Inferred from Sequence Orthology more info
involved_in auditory behavior	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within auditory behavior	ISO Inferred from Sequence Orthology more info
involved_in cell morphogenesis involved in neuron differentiation	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within cell morphogenesis involved in neuron differentiation	ISO Inferred from Sequence Orthology more info
involved_in cellular response to cocaine	IEA Inferred from Electronic Annotation more info
involved_in cellular response to cocaine	ISO Inferred from Sequence Orthology more info
involved_in chloride transmembrane transport	IEA Inferred from Electronic Annotation more info
involved_in chloride transmembrane transport	ISO Inferred from Sequence Orthology more info
involved_in chloride transmembrane transport	ISS Inferred from Sequence or Structural Similarity more info
involved_in cranial nerve development	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within cranial nerve development	ISO Inferred from Sequence Orthology more info
involved_in gamma-aminobutyric acid biosynthetic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within gamma-aminobutyric acid biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in intracellular sodium ion homeostasis	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within intracellular sodium ion homeostasis	ISO Inferred from Sequence Orthology more info
involved_in malate-aspartate shuttle	IDA Inferred from Direct Assay more info	PubMed
involved_in neuromuscular process controlling balance	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within neuromuscular process controlling balance	ISO Inferred from Sequence Orthology more info
involved_in neurotransmitter transport	TAS Traceable Author Statement more info	PubMed
involved_in neutral amino acid transport	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of synaptic transmission	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within positive regulation of synaptic transmission	ISO Inferred from Sequence Orthology more info
involved_in potassium ion transmembrane transport	IEA Inferred from Electronic Annotation more info
involved_in potassium ion transmembrane transport	ISO Inferred from Sequence Orthology more info
involved_in potassium ion transmembrane transport	ISS Inferred from Sequence or Structural Similarity more info
involved_in response to antibiotic	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within response to antibiotic	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within response to inorganic substance	IDA Inferred from Direct Assay more info	PubMed
involved_in response to light stimulus	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within response to light stimulus	ISO Inferred from Sequence Orthology more info
involved_in response to wounding	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within response to wounding	ISO Inferred from Sequence Orthology more info
involved_in response to xenobiotic stimulus	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within response to xenobiotic stimulus	ISO Inferred from Sequence Orthology more info
involved_in sensory perception of sound	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within sensory perception of sound	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in basal plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in basolateral plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in cell periphery	ISO Inferred from Sequence Orthology more info
located_in cell projection	ISO Inferred from Sequence Orthology more info
located_in cell surface	IEA Inferred from Electronic Annotation more info
located_in cell surface	ISO Inferred from Sequence Orthology more info
located_in dendritic spine	IDA Inferred from Direct Assay more info	PubMed
part_of membrane protein complex	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrial inner membrane	IDA Inferred from Direct Assay more info	PubMed
located_in neuron projection	IDA Inferred from Direct Assay more info	PubMed
located_in neuron projection	IEA Inferred from Electronic Annotation more info
located_in neuron projection	ISO Inferred from Sequence Orthology more info
located_in neuronal cell body	IDA Inferred from Direct Assay more info	PubMed
located_in neuronal cell body	IEA Inferred from Electronic Annotation more info
located_in neuronal cell body	ISO Inferred from Sequence Orthology more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	IEA Inferred from Electronic Annotation more info
located_in plasma membrane	IMP Inferred from Mutant Phenotype more info	PubMed
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	ISS Inferred from Sequence or Structural Similarity more info
located_in synapse	IEA Inferred from Electronic Annotation more info
located_in synapse	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: excitatory amino acid transporter 1

Names: glial glutamate transporter; glutamate transporter; glutamate/aspartate transporter; sodium-dependent glutamate/aspartate transporter 1; solute carrier family 1 (glial high affinity glutamate transporter), member 3

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001289941.1 → NP_001276870.1 excitatory amino acid transporter 1 isoform 2

See identical proteins and their annotated locations for NP_001276870.1

Status: VALIDATED

Description

Transcript Variant: This variant (Glast1a, PMID:11086157) lacks an alternate in-frame exon in the 5' coding region, compared to variant Glast. The encoded protein (isoform 2) is shorter, compared to isoform 1.

Source sequence(s)

AF265360, FM094687, FQ082713, S59158

UniProtKB/Swiss-Prot

P24942, Q9JK43

UniProtKB/TrEMBL

A0A0G2JSU1, A6KGI5

Related

ENSRNOP00000036554.4, ENSRNOT00000035821.6

Conserved Domains (1) summary

pfam00375
Location:45 → 451

SDF; Sodium:dicarboxylate symporter family
NM_001289942.1 → NP_001276871.1 excitatory amino acid transporter 1 isoform 3

Status: VALIDATED

Description

Transcript Variant: This variant (Glast1b) lacks an alternate in-frame exon in the 3' coding region, compared to variant Glast. The encoded protein (isoform 3) is shorter, compared to isoform 1.

Source sequence(s)

AF265360, FM094687, FQ082713, FQ097869, S59158

UniProtKB/Swiss-Prot

P24942, Q9JK43

UniProtKB/TrEMBL

A0A0G2KAS7

Related

ENSRNOP00000075509.1, ENSRNOT00000085599.2

Conserved Domains (1) summary

pfam00375
Location:50 → 452

SDF; Sodium:dicarboxylate symporter family
NM_001289943.1 → NP_001276872.1 excitatory amino acid transporter 1 isoform 4

Status: VALIDATED

Description

Transcript Variant: This variant (Glast1c, PMID:22026960) lacks two alternate in-frame exons in the coding region, compared to variant Glast. The encoded protein (isoform 4) is shorter, compared to isoform 1.

Source sequence(s)

AF265360, FM094687, FQ082713, FQ097869, S59158

UniProtKB/TrEMBL

A0A0G2K611

Related

ENSRNOP00000073634.1, ENSRNOT00000077790.2

Conserved Domains (1) summary

pfam00375
Location:50 → 385

SDF; Sodium:dicarboxylate symporter family
NM_019225.2 → NP_062098.1 excitatory amino acid transporter 1 isoform 1

See identical proteins and their annotated locations for NP_062098.1

Status: VALIDATED

Description

Transcript Variant: This variant (Glast) represents the longest transcript and encodes the longest isoform (1).

Source sequence(s)

AF265360, FM094687, FQ097869, S59158

UniProtKB/Swiss-Prot

P24942, Q9JK43

UniProtKB/TrEMBL

A6KGI6, G3V846

Related

ENSRNOP00000022319.3, ENSRNOT00000022319.7

Conserved Domains (1) summary

pfam00375
Location:50 → 497

SDF; Sodium:dicarboxylate symporter family