F2 coagulation factor II [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: F2provided by MGI
Official Full Name: coagulation factor IIprovided by MGI
Primary source: MGI:MGI:88380
See related: Ensembl:ENSMUSG00000027249 AllianceGenome:MGI:88380
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Cf2; FII; Cf-2
Summary: This gene encodes a vitamin K-dependent glycoprotein coagulation factor that plays an important role in the process of blood coagulation and hemostasis. The encoded protein is an inactive zymogen that undergoes enzymatic cleavage by the coagulation factor Xa to form an active serine protease that converts soluble fibrinogen to insoluble fibrin clot. Most of the mice lacking the encoded protein die at an embryonic stage due to defects in yolk sac vasculature, while the rare nenonates succumb to hemorrhage on the first postnatal day. [provided by RefSeq, Apr 2015]
Expression: Biased expression in liver adult (RPKM 695.9), liver E18 (RPKM 621.7) and 3 other tissues See more
Orthologs: human all
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Genomic context

Location:: 2 E1; 2 50.63 cM

Exon count:: 16

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	2	NC_000068.8 (91442742..91466799, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	2	NC_000068.7 (91612397..91636457, complement)

Chromosome 2 - NC_000068.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Thrombin-activated interleukin-1alpha drives atherogenesis, but also promotes vascular smooth muscle cell proliferation and collagen production.
Title: Thrombin-activated interleukin-1α drives atherogenesis, but also promotes vascular smooth muscle cell proliferation and collagen production.
Thrombin-Induced Microglia Activation Modulated through Aryl Hydrocarbon Receptors.
Title: Thrombin-Induced Microglia Activation Modulated through Aryl Hydrocarbon Receptors.
Thrombogenic potential of picomolar coagulation factor XIa is mediated by thrombin wave propagation.
Title: Thrombogenic potential of picomolar coagulation factor XIa is mediated by thrombin wave propagation.
Ischemic stroke in PAR1 KO mice: Decreased brain plasmin and thrombin activity along with decreased infarct volume.
Title: Ischemic stroke in PAR1 KO mice: Decreased brain plasmin and thrombin activity along with decreased infarct volume.
Syndecan-4 Protects the Heart From the Profibrotic Effects of Thrombin-Cleaved Osteopontin.
Title: Syndecan-4 Protects the Heart From the Profibrotic Effects of Thrombin-Cleaved Osteopontin.
Data show that bone marrow kinase on the X chromosome (BMX) specifically repressed thrombin-PAR1 (protease-activated receptor-1) signaling in endothelial cells by directly phosphorylating PAR1 and promoting its internalization and deactivation.
Title: BMX Represses Thrombin-PAR1-Mediated Endothelial Permeability and Vascular Leakage During Early Sepsis.
Thrombin induced VEGF release from astrocytes require p44/42. Thrombin induced VEGF release from astrocytes is reduced in PAR-1 knockout mice. Thrombin induced p44/42 activation in astrocytes is blocked in PAR-1 knockout mice.
Title: Astrocytic thrombin-evoked VEGF release is dependent on p44/42 MAPKs and PAR1.
These results suggested that the thrombinstimulated synthesis of IL6 was limited by HSP90 in osteoblasts, and that the effects of HSP90 were exerted at the point between Rhokinase and p38 MAPK.
Title: HSP90 limits thrombin‑stimulated IL‑6 synthesis in osteoblast‑like MC3T3‑E1 cells: Regulation of p38 MAPK.
Platelet derived microparticles formed in sepsis are a potent inducer of thrombin generation via phosphatidylserine exposure, and activation of both the intrinsic and extrinsic pathway of coagulation.
Title: Platelet-derived microparticles regulates thrombin generation via phophatidylserine in abdominal sepsis.
These findings imply that a thrombin-pericyte interaction in the brain could be a significant mechanism responsible for blood-brain barrier dysfunction under obesity-associated diabetic conditions and thus could represent a therapeutic target for central nervous system complications of diabetes.
Title: Contribution of thrombin-reactive brain pericytes to blood-brain barrier dysfunction in an in vivo mouse model of obesity-associated diabetes and an in vitro rat model.

Submit: New GeneRIF Correction See all GeneRIFs (47)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (6) 1 citation
Endonuclease-mediated (1)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

PMC22695P1 (e-PCR)
- UniSTS:272145

F2 (e-PCR), detects polymorphism
- UniSTS:525904

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables calcium ion binding	IEA Inferred from Electronic Annotation more info
enables endopeptidase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables heparin binding	ISO Inferred from Sequence Orthology more info
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables lipopolysaccharide binding	ISO Inferred from Sequence Orthology more info
enables peptidase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables serine-type endopeptidase activity	IBA Inferred from Biological aspect of Ancestor more info
enables serine-type endopeptidase activity	IDA Inferred from Direct Assay more info	PubMed
enables serine-type endopeptidase activity	ISO Inferred from Sequence Orthology more info
enables serine-type peptidase activity	IEA Inferred from Electronic Annotation more info
enables signaling receptor binding	ISO Inferred from Sequence Orthology more info
enables thrombospondin receptor activity	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in G protein-coupled receptor signaling pathway	IDA Inferred from Direct Assay more info	PubMed
involved_in G protein-coupled receptor signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within acute-phase response	IEA Inferred from Electronic Annotation more info
involved_in antimicrobial humoral immune response mediated by antimicrobial peptide	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within blood coagulation	IEA Inferred from Electronic Annotation more info
involved_in blood coagulation	IEA Inferred from Electronic Annotation more info
involved_in cell surface receptor signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in cytolysis by host of symbiont cells	ISO Inferred from Sequence Orthology more info
involved_in fibrinolysis	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within hemostasis	IEA Inferred from Electronic Annotation more info
involved_in ligand-gated ion channel signaling pathway	IDA Inferred from Direct Assay more info	PubMed
involved_in ligand-gated ion channel signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of astrocyte differentiation	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of cytokine production involved in inflammatory response	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of proteolysis	ISO Inferred from Sequence Orthology more info
involved_in neutrophil-mediated killing of gram-negative bacterium	ISO Inferred from Sequence Orthology more info
involved_in platelet activation	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within platelet activation	IDA Inferred from Direct Assay more info	PubMed
involved_in platelet activation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of blood coagulation	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of blood coagulation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within positive regulation of cell growth	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within positive regulation of cell population proliferation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of collagen biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of insulin secretion	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of insulin secretion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of lipid kinase activity	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of phospholipase C-activating G protein-coupled receptor signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of protein localization to nucleus	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of protein phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of reactive oxygen species metabolic process	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of release of sequestered calcium ion into cytosol	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within proteolysis	IEA Inferred from Electronic Annotation more info
involved_in proteolysis	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within regulation of cell shape	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of cytosolic calcium ion concentration	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within regulation of gene expression	IDA Inferred from Direct Assay more info	PubMed
involved_in response to wounding	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within thrombin-activated receptor signaling pathway	ISO Inferred from Sequence Orthology more info	PubMed

Component	Evidence Code	Pubs
located_in collagen-containing extracellular matrix	HDA more info	PubMed
is_active_in collagen-containing extracellular matrix	IBA Inferred from Biological aspect of Ancestor more info
located_in collagen-containing extracellular matrix	ISO Inferred from Sequence Orthology more info
colocalizes_with external side of plasma membrane	ISO Inferred from Sequence Orthology more info
located_in extracellular region	IEA Inferred from Electronic Annotation more info
is_active_in extracellular space	IBA Inferred from Biological aspect of Ancestor more info
located_in extracellular space	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: prothrombin

Names: thrombin

NP_034298.1

EC 3.4.21.5

XP_011237590.1

EC 3.4.21.5

XP_030103259.1

EC 3.4.21.5

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_010168.4 → NP_034298.1 prothrombin preproprotein

See identical proteins and their annotated locations for NP_034298.1

Status: REVIEWED

Source sequence(s)

AL691489

Consensus CDS

CCDS16434.1

UniProtKB/Swiss-Prot

P19221

UniProtKB/TrEMBL

H7BX99, Q3TJ94

Related

ENSMUSP00000028681.9, ENSMUST00000028681.15

Conserved Domains (5) summary

smart00020
Location:360 → 610

Tryp_SPc; Trypsin-like serine protease

smart00069
Location:25 → 89

GLA; Domain containing Gla (gamma-carboxyglutamate) residues

smart00130
Location:107 → 189

KR; Kringle domain

cd00190
Location:361 → 613

Tryp_SPc; Trypsin-like serine protease; Many of these are synthesized as inactive precursor zymogens that are cleaved during limited proteolysis to generate their active forms. Alignment contains also inactive enzymes that have substitutions of the catalytic triad ...

pfam09396
Location:313 → 360

Thrombin_light; Thrombin light chain

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000068.8 Reference GRCm39 C57BL/6J

Range

91442742..91466799 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_030247399.1 → XP_030103259.1 prothrombin isoform X1

UniProtKB/TrEMBL

H7BX99

Conserved Domains (4) summary

smart00020
Location:360 → 575

Tryp_SPc; Trypsin-like serine protease

smart00069
Location:25 → 89

GLA; Domain containing Gla (gamma-carboxyglutamate) residues

smart00130
Location:107 → 189

KR; Kringle domain

pfam09396
Location:314 → 360

Thrombin_light; Thrombin light chain
XM_011239288.2 → XP_011237590.1 prothrombin isoform X1

UniProtKB/TrEMBL

H7BX99

Conserved Domains (4) summary

smart00020
Location:360 → 575

Tryp_SPc; Trypsin-like serine protease

smart00069
Location:25 → 89

GLA; Domain containing Gla (gamma-carboxyglutamate) residues

smart00130
Location:107 → 189

KR; Kringle domain

pfam09396
Location:314 → 360

Thrombin_light; Thrombin light chain