Adh5 alcohol dehydrogenase 5 (class III), chi polypeptide [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Adh5provided by MGI
Official Full Name: alcohol dehydrogenase 5 (class III), chi polypeptideprovided by MGI
Primary source: MGI:MGI:87929
See related: Ensembl:ENSMUSG00000028138 AllianceGenome:MGI:87929
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Adh3; Adh-5; GSNOR
Summary: Enables S-(hydroxymethyl)glutathione dehydrogenase activity; alcohol dehydrogenase (NAD+) activity; and identical protein binding activity. Acts upstream of or within several processes, including formaldehyde catabolic process; peptidyl-cysteine S-nitrosylation; and response to lipopolysaccharide. Located in mitochondrion. Is expressed in several structures, including alimentary system; central nervous system; genitourinary system; integumental system; and respiratory system. Human ortholog(s) of this gene implicated in AMED syndrome and asthma. Orthologous to human ADH5 (alcohol dehydrogenase 5 (class III), chi polypeptide). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Ubiquitous expression in liver E18 (RPKM 86.3), CNS E11.5 (RPKM 59.7) and 25 other tissues See more
Orthologs: human all
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Genomic context

Location:: 3 G3; 3 64.26 cM

Exon count:: 10

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	3	NC_000069.7 (138142961..138161260)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	3	NC_000069.6 (138437200..138455499)

Chromosome 3 - NC_000069.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Mitochondrial GSNOR Alleviates Cardiac Dysfunction via ANT1 Denitrosylation.
Title: Mitochondrial GSNOR Alleviates Cardiac Dysfunction via ANT1 Denitrosylation.
Class III Alcohol Dehydrogenase Plays a Key Role in the Onset of Alcohol-Related/-Associated Liver Disease as an S-Nitrosoglutathione Reductase in Mice.
Title: Class III Alcohol Dehydrogenase Plays a Key Role in the Onset of Alcohol-Related/-Associated Liver Disease as an S-Nitrosoglutathione Reductase in Mice.
S-Nitrosoglutathione Reductase Deficiency Causes Aberrant Placental S-Nitrosylation and Preeclampsia.
Title: S-Nitrosoglutathione Reductase Deficiency Causes Aberrant Placental S-Nitrosylation and Preeclampsia.
ADH5-mediated NO bioactivity maintains metabolic homeostasis in brown adipose tissue.
Title: ADH5-mediated NO bioactivity maintains metabolic homeostasis in brown adipose tissue.
GSNOR facilitates antiviral innate immunity by restricting TBK1 cysteine S-nitrosation.
Title: GSNOR facilitates antiviral innate immunity by restricting TBK1 cysteine S-nitrosation.
Data show that aldo-keto reductase family 1 member A1 (AKR1A1) deletion from murine tissues dramatically lowered NADH-dependent GSNOR activity..
Title: AKR1A1 is a novel mammalian S-nitroso-glutathione reductase.
nNOS/GSNOR interaction contributes to skeletal muscle differentiation and homeostasis in humans and mice.
Title: nNOS/GSNOR interaction contributes to skeletal muscle differentiation and homeostasis.
Our data indicates that nebivolol has no effect on GSNOR activity, nor does nebivolol inhibit thioredoxin reductase, two of the major protein denitrosylases.
Title: Hiding in Plain Sight: Nebivolol Exhibits Compelling Tocolytic Properties.
Site-directed mutagenesis of Akt at Cys224 revealed that S-nitrosylation at this site was pivotal for the reduced phosphorylation at Akt Ser473, which led to impaired Akt signaling. Furthermore, on HHcy challenge, as compared with GSNOR(+/+)ApoE(-/-) littermate controls, GSNOR(-/-)ApoE(-/-) double knockout mice showed reduced T-cell activation with concurrent reduction of atherosclerosis.
Title: GSNOR modulates hyperhomocysteinemia-induced T cell activation and atherosclerosis by switching Akt S-nitrosylation to phosphorylation.
Data (including data from studies using knockout and transgenic mice) suggest that obesity and diabetes are accompanied by decreases in GSNOR activity in hepatocytes engendering nitrosative stress; obesity promotes S-nitrosylation of lysosomal proteins in liver, thereby impairing lysosomal enzyme activities and compromising autophagy.
Title: S-Nitrosoglutathione Reductase Dysfunction Contributes to Obesity-Associated Hepatic Insulin Resistance via Regulating Autophagy.

Submit: New GeneRIF Correction See all GeneRIFs (34)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Gene trapped (2)
Targeted (7) 1 citation
Endonuclease-mediated (4)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

U48970 (e-PCR)
- UniSTS:125758

Adh5 (e-PCR), detects polymorphism
- UniSTS:546634

Adh5 (e-PCR), detects polymorphism
- UniSTS:461946

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables S-(hydroxymethyl)glutathione dehydrogenase (NAD(P)+) activity	IBA Inferred from Biological aspect of Ancestor more info
enables S-(hydroxymethyl)glutathione dehydrogenase (NAD(P)+) activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables S-(hydroxymethyl)glutathione dehydrogenase (NAD+) activity	IEA Inferred from Electronic Annotation more info
enables S-(hydroxymethyl)glutathione dehydrogenase (NADP+) activity	IEA Inferred from Electronic Annotation more info
enables alcohol dehydrogenase (NAD+) activity	IDA Inferred from Direct Assay more info	PubMed
enables alcohol dehydrogenase (NAD+) activity	ISO Inferred from Sequence Orthology more info
enables alcohol dehydrogenase (NAD+) activity, zinc-dependent	IBA Inferred from Biological aspect of Ancestor more info
enables fatty acid binding	ISO Inferred from Sequence Orthology more info
enables formaldehyde dehydrogenase activity	ISO Inferred from Sequence Orthology more info
enables identical protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables oxidoreductase activity	IEA Inferred from Electronic Annotation more info
enables zinc ion binding	IBA Inferred from Biological aspect of Ancestor more info
enables zinc ion binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in ethanol catabolic process	ISO Inferred from Sequence Orthology more info
involved_in fatty acid omega-oxidation	ISO Inferred from Sequence Orthology more info
involved_in formaldehyde catabolic process	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within formaldehyde catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within lipid metabolic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within peptidyl-cysteine S-nitrosylation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within positive regulation of blood pressure	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within respiratory system process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within response to lipopolysaccharide	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within response to nitrosative stress	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to redox state	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within retinoid metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
located_in mitochondrion	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: alcohol dehydrogenase class-3

Names: ADH-B2; FALDH; FDH; GSH-FDH; S-(hydroxymethyl)glutathione dehydrogenase; S-nitrosoglutathione reductase; alcohol dehydrogenase 2; alcohol dehydrogenase B2; alcohol dehydrogenase class-III; glutathione-dependent formaldehyde dehydrogenase

NP_001275507.1

EC 1.1.1.1

EC 1.1.1.284

NP_031436.2

EC 1.1.1.1

EC 1.1.1.284

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001288578.1 → NP_001275507.1 alcohol dehydrogenase class-3 isoform 2

Status: VALIDATED

Description

Transcript Variant: This variant (2) has an alternate 5' exon, which results in translation intiation at a downstream AUG start codon, compared to variant 1. The resulting isoform (2) has a shorter N-terminus, compared to isoform 1.

Source sequence(s)

AK076507, CF172597

UniProtKB/Swiss-Prot

P28474

Conserved Domains (1) summary

cl16912
Location:1 → 251

MDR; Medium chain reductase/dehydrogenase (MDR)/zinc-dependent alcohol dehydrogenase-like family
NM_007410.3 → NP_031436.2 alcohol dehydrogenase class-3 isoform 1

See identical proteins and their annotated locations for NP_031436.2

Status: VALIDATED

Description

Transcript Variant: This variant (1) encodes the longer isoform (1).

Source sequence(s)

AK076507, BY125542

Consensus CDS

CCDS17868.1

UniProtKB/Swiss-Prot

P28474, Q3TW83, Q8C662

UniProtKB/TrEMBL

Q6P5I3

Related

ENSMUSP00000005964.3, ENSMUST00000005964.7

Conserved Domains (2) summary

cd08300
Location:6 → 373

alcohol_DH_class_III; class III alcohol dehydrogenases

COG1062
Location:6 → 374

FrmA; Zn-dependent alcohol dehydrogenase [General function prediction only]