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    Spc25 SPC25, NDC80 kinetochore complex component, homolog (S. cerevisiae) [ Mus musculus (house mouse) ]

    Gene ID: 66442, updated on 5-Mar-2024

    Summary

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    Official Symbol
    Spc25provided by MGI
    Official Full Name
    SPC25, NDC80 kinetochore complex component, homolog (S. cerevisiae)provided by MGI
    Primary source
    MGI:MGI:1913692
    See related
    Ensembl:ENSMUSG00000005233 AllianceGenome:MGI:1913692
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Mus musculus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
    Also known as
    Spbc25; 2600017H08Rik; 2610205L13Rik
    Summary
    This gene encodes a component of the kinetochore-associated NDC80 protein complex, which is required for the mitotic spindle checkpoint and for microtubule-kinetochore attachment. During meiosis in mouse, the protein localizes to the germinal vesicle and then is associated with the chromosomes following germinal vesicle breakdown. Knockdown of this gene in oocytes results in precocious polar body extrusion, chromosome misalignment and aberrant spindle formation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015]
    Expression
    Biased expression in liver E14 (RPKM 25.3), CNS E11.5 (RPKM 24.7) and 10 other tissues See more
    Orthologs
    human all
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    Genomic context

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    Location:
    2 C2; 2 39.64 cM
    Exon count:
    9
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCm39 (GCF_000001635.27) 2 NC_000068.8 (69009753..69036583, complement)
    108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 2 NC_000068.7 (69193895..69206213, complement)

    Chromosome 2 - NC_000068.8Genomic Context describing neighboring genes Neighboring gene ceramide synthase 6 Neighboring gene predicted gene, 52515 Neighboring gene STARR-seq mESC enhancer starr_04722 Neighboring gene STARR-seq mESC enhancer starr_04727 Neighboring gene predicted gene, 39840 Neighboring gene nitric oxide synthase trafficker Neighboring gene STARR-positive B cell enhancer ABC_E412 Neighboring gene STARR-seq mESC enhancer starr_04729 Neighboring gene glucose-6-phosphatase, catalytic, 2 Neighboring gene STARR-seq mESC enhancer starr_04731 Neighboring gene ATP-binding cassette, sub-family B member 11 Neighboring gene STARR-seq mESC enhancer starr_04732

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: Mouse ENCODE transcriptome data
    • Description: RNA profiling data sets generated by the Mouse ENCODE project.
    • BioProject: PRJNA66167
    • Publication: PMID 25409824
    • Analysis date: n/a

    Bibliography

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    Related articles in PubMed

    1. Microtubule attachment and spindle assembly checkpoint signalling at the kinetochore. Foley EA, et al. Nat Rev Mol Cell Biol, 2013 Jan. PMID 23258294, Free PMC Article
    2. Perturbation of Spc25 expression affects meiotic spindle organization, chromosome alignment and spindle assembly checkpoint in mouse oocytes. Sun SC, et al. Cell Cycle, 2010 Nov 15. PMID 21084868
    3. Implications for kinetochore-microtubule attachment from the structure of an engineered Ndc80 complex. Ciferri C, et al. Cell, 2008 May 2. PMID 18455984, Free PMC Article
    4. BCAS2 is involved in alternative splicing and mouse oocyte development. Zhang J, et al. FASEB J, 2022 Feb. PMID 34972250
    5. The KMN protein network--chief conductors of the kinetochore orchestra. Varma D, et al. J Cell Sci, 2012 Dec 15. PMID 23418356, Free PMC Article

    See all (28) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Spc25 is required for chromosome alignment, spindle formation, and proper spindle checkpoint signaling during meiosis.
      Title: Perturbation of Spc25 expression affects meiotic spindle organization, chromosome alignment and spindle assembly checkpoint in mouse oocytes.
    Submit: New GeneRIF Correction

    Variation

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    Alleles

    Alleles of this type are documented at Mouse Genome Informatics  (MGI)
    • Endonuclease-mediated (2) 

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Markers

    Homology

    Gene Ontology Provided by MGI

    Function Evidence Code Pubs
    enables molecular_function ND
    No biological Data available
    more info
    		  
    Process Evidence Code Pubs
    involved_in attachment of spindle microtubules to kinetochore ISO
    Inferred from Sequence Orthology
    more info
    		  
    acts_upstream_of_or_within cell cycle IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within cell division IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in chromosome segregation IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in chromosome segregation ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in chromosome segregation NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in mitotic spindle assembly checkpoint signaling NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in mitotic spindle organization ISO
    Inferred from Sequence Orthology
    more info
    		  
    Component Evidence Code Pubs
    part_of Ndc80 complex IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of Ndc80 complex ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in chromosome IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in chromosome, centromeric region IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in cytosol ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in kinetochore ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in kinetochore NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    located_in nucleus IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    kinetochore protein Spc25
    Names
    spindle pole body component 25 homolog

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001199123.2 → NP_001186052.1  kinetochore protein Spc25 isoform 1

      See identical proteins and their annotated locations for NP_001186052.1

      Status: REVIEWED

      Description
      Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). Variants 1, 2, and 3 encode the same isoform.
      Source sequence(s)
      AL929221, BC033605, CJ086993
      Consensus CDS
      CCDS57174.1
      UniProtKB/Swiss-Prot
      Q3UA16, Q9D021, Q9D1K6
      UniProtKB/TrEMBL
      A2AUT4
      Related
      ENSMUSP00000107939.2, ENSMUST00000112320.8
      Conserved Domains (2) summary
      COG4942
      Location:55 → 129
      EnvC; Septal ring factor EnvC, activator of murein hydrolases AmiA and AmiB [Cell cycle control, cell division, chromosome partitioning]
      pfam08234
      Location:151 → 220
      Spindle_Spc25; Chromosome segregation protein Spc25

    2. NM_001199124.2 → NP_001186053.1  kinetochore protein Spc25 isoform 1

      See identical proteins and their annotated locations for NP_001186053.1

      Status: REVIEWED

      Description
      Transcript Variant: This variant (3) uses an alternate splice site in the 5' UTR compared to variant 1. Variants 1, 2, and 3 encode the same isoform (1).
      Source sequence(s)
      AL929221, BC027121, CJ086993, CX222514
      Consensus CDS
      CCDS57174.1
      UniProtKB/Swiss-Prot
      Q3UA16, Q9D021, Q9D1K6
      UniProtKB/TrEMBL
      A2AUT4
      Related
      ENSMUSP00000005365.9, ENSMUST00000005365.15
      Conserved Domains (2) summary
      COG4942
      Location:55 → 129
      EnvC; Septal ring factor EnvC, activator of murein hydrolases AmiA and AmiB [Cell cycle control, cell division, chromosome partitioning]
      pfam08234
      Location:151 → 220
      Spindle_Spc25; Chromosome segregation protein Spc25

    3. NM_001305800.1 → NP_001292729.1  kinetochore protein Spc25 isoform 1

      See identical proteins and their annotated locations for NP_001292729.1

      Status: REVIEWED

      Description
      Transcript Variant: This variant (2) uses an alternate splice site in the 5' UTR compared to variant 1. Variants 1, 2, and 3 encode the same isoform (1).
      Source sequence(s)
      AK146727, AL929221, BC033605, CJ086993
      Consensus CDS
      CCDS57174.1
      UniProtKB/Swiss-Prot
      Q3UA16, Q9D021, Q9D1K6
      UniProtKB/TrEMBL
      A2AUT4
      Conserved Domains (2) summary
      COG4942
      Location:55 → 129
      EnvC; Septal ring factor EnvC, activator of murein hydrolases AmiA and AmiB [Cell cycle control, cell division, chromosome partitioning]
      pfam08234
      Location:151 → 220
      Spindle_Spc25; Chromosome segregation protein Spc25

    4. NM_025565.4 → NP_079841.1  kinetochore protein Spc25 isoform 2

      See identical proteins and their annotated locations for NP_079841.1

      Status: REVIEWED

      Description
      Transcript Variant: This variant (4) differs in the 5' UTR and lacks an exon in the 5' coding region, which results in use of a downstream start codon compared to variant 1. It encodes isoform (2), which has a shorter N-terminus compared to isoform 1.
      Source sequence(s)
      AK003408, AL929221, BC033605, CJ086993, CX222514
      Consensus CDS
      CCDS16088.1
      UniProtKB/TrEMBL
      A2AUT4
      Related
      ENSMUSP00000128039.3, ENSMUST00000167875.9
      Conserved Domains (1) summary
      pfam08234
      Location:103 → 172
      Spindle_Spc25; Chromosome segregation protein Spc25

    RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCm39 C57BL/6J

    Genomic

    1. NC_000068.8 Reference GRCm39 C57BL/6J

      Range
      69009753..69036583 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_036162451.1 → XP_036018344.1  kinetochore protein Spc25 isoform X2

      UniProtKB/TrEMBL
      A2AUT3
      Conserved Domains (1) summary
      COG4942
      Location:55 → 129
      EnvC; Septal ring factor EnvC, activator of murein hydrolases AmiA and AmiB [Cell cycle control, cell division, chromosome partitioning]

    2. XM_006500003.4 → XP_006500066.1  kinetochore protein Spc25 isoform X1

      See identical proteins and their annotated locations for XP_006500066.1

      UniProtKB/Swiss-Prot
      Q3UA16, Q9D021, Q9D1K6
      UniProtKB/TrEMBL
      A2AUT4
      Conserved Domains (2) summary
      COG4942
      Location:55 → 129
      EnvC; Septal ring factor EnvC, activator of murein hydrolases AmiA and AmiB [Cell cycle control, cell division, chromosome partitioning]
      pfam08234
      Location:151 → 220
      Spindle_Spc25; Chromosome segregation protein Spc25

    3. XM_036162450.1 → XP_036018343.1  kinetochore protein Spc25 isoform X1

      UniProtKB/Swiss-Prot
      Q3UA16, Q9D021, Q9D1K6
      UniProtKB/TrEMBL
      A2AUT4
      Conserved Domains (2) summary
      COG4942
      Location:55 → 129
      EnvC; Septal ring factor EnvC, activator of murein hydrolases AmiA and AmiB [Cell cycle control, cell division, chromosome partitioning]
      pfam08234
      Location:151 → 220
      Spindle_Spc25; Chromosome segregation protein Spc25
    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q3UA16.1 GenPept UniProtKB/Swiss-Prot:Q3UA16

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