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    G2e3 G2/M-phase specific E3 ubiquitin ligase [ Mus musculus (house mouse) ]

    Gene ID: 217558, updated on 11-Apr-2024

    Summary

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    Official Symbol
    G2e3provided by MGI
    Official Full Name
    G2/M-phase specific E3 ubiquitin ligaseprovided by MGI
    Primary source
    MGI:MGI:2444298
    See related
    Ensembl:ENSMUSG00000035293 AllianceGenome:MGI:2444298
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Mus musculus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
    Also known as
    mKIAA1333; 9030416F18; 6030408C04Rik; D930034K21Rik
    Summary
    Enables ubiquitin protein ligase activity. Acts upstream of or within blastocyst development; negative regulation of intrinsic apoptotic signaling pathway; and protein polyubiquitination. Predicted to be located in Golgi apparatus and cytosol. Predicted to be active in nucleus. Is expressed in several structures, including central nervous system; epithelium; limb; liver; and male reproductive gland or organ. Orthologous to human G2E3 (G2/M-phase specific E3 ubiquitin protein ligase). [provided by Alliance of Genome Resources, Apr 2022]
    Expression
    Broad expression in CNS E11.5 (RPKM 7.7), CNS E14 (RPKM 6.0) and 17 other tissues See more
    Orthologs
    human all
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    Genomic context

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    Location:
    12 B3; 12 22.11 cM
    Exon count:
    16
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCm39 (GCF_000001635.27) 12 NC_000078.7 (51395013..51423769)
    108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 12 NC_000078.6 (51348230..51376986)

    Chromosome 12 - NC_000078.7Genomic Context describing neighboring genes Neighboring gene predicted gene, 34745 Neighboring gene tubulin, alpha 1B pseudogene Neighboring gene STARR-seq mESC enhancer starr_32098 Neighboring gene STARR-seq mESC enhancer starr_32099 Neighboring gene STARR-seq mESC enhancer starr_32100 Neighboring gene ribosomal protein S11, pseudogene 4 Neighboring gene STARR-seq mESC enhancer starr_32102 Neighboring gene STARR-seq mESC enhancer starr_32103 Neighboring gene predicted gene, 40422 Neighboring gene Sec1 family domain containing 1 Neighboring gene high mobility group protein 1 pseudogene Neighboring gene zyg-11 homolog A pseudogene

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: Mouse ENCODE transcriptome data
    • Description: RNA profiling data sets generated by the Mouse ENCODE project.
    • BioProject: PRJNA66167
    • Publication: PMID 25409824
    • Analysis date: n/a

    Bibliography

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    Related articles in PubMed

    1. Obesity of G2e3 Knockout Mice Suggests That Obesity-Associated Variants Near Human G2E3 Decrease G2E3 Activity. Powell DR, et al. Diabetes Metab Syndr Obes, 2020. PMID 32801815, Free PMC Article
    2. G2E3 is a dual function ubiquitin ligase required for early embryonic development. Brooks WS, et al. J Biol Chem, 2008 Aug 8. PMID 18511420, Free PMC Article
    3. A genomic atlas of mouse hypothalamic development. Shimogori T, et al. Nat Neurosci, 2010 Jun. PMID 20436479, Free PMC Article
    4. Prediction of the coding sequences of mouse homologues of KIAA gene: III. the complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries. Okazaki N, et al. DNA Res, 2003 Aug 31. PMID 14621295
    5. Construction of long-transcript enriched cDNA libraries from submicrogram amounts of total RNAs by a universal PCR amplification method. Piao Y, et al. Genome Res, 2001 Sep. PMID 11544199, Free PMC Article

    See all (24) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. G2E3 is a dual function ubiquitin ligase essential for prevention of apoptosis in early embryogenesis
      Title: G2E3 is a dual function ubiquitin ligase required for early embryonic development.
    Submit: New GeneRIF Correction

    Variation

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    Alleles

    Alleles of this type are documented at Mouse Genome Informatics  (MGI)
    • Gene trapped (2)  1 citation
    • Endonuclease-mediated (2) 

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Markers

    Homology

    Gene Ontology Provided by MGI

    Function Evidence Code Pubs
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables transferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ubiquitin protein ligase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables ubiquitin-protein transferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    acts_upstream_of_or_within apoptotic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within blastocyst development IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within multicellular organism development IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within negative regulation of intrinsic apoptotic signaling pathway IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within protein polyubiquitination IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in Golgi apparatus ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in cytosol ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in intracellular membrane-bounded organelle ISO
    Inferred from Sequence Orthology
    more info
    		  
    is_active_in nucleus IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  

    General protein information

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    Preferred Names
    G2/M phase-specific E3 ubiquitin-protein ligase
    Names
    G2/M phase-specific HECT-type E3 ubiquitin transferase
    NP_001015099.2
    NP_001161435.1
    NP_001161436.1

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001015099.2 → NP_001015099.2  G2/M phase-specific E3 ubiquitin-protein ligase isoform 1

      See identical proteins and their annotated locations for NP_001015099.2

      Status: VALIDATED

      Description
      Transcript Variant: This variant (1) represents the predominant transcript, and encodes isoform 1.
      Source sequence(s)
      AA231622, AK086526, AK129332, BY077085, CJ239277
      Consensus CDS
      CCDS25900.1
      UniProtKB/Swiss-Prot
      E9QK24, Q5RJY2, Q6ZPT7, Q8BNA4
      Related
      ENSMUSP00000054474.7, ENSMUST00000054308.13
      Conserved Domains (4) summary
      smart00119
      Location:388 → 690
      HECTc; Domain Homologous to E6-AP Carboxyl Terminus with
      cd15496
      Location:232 → 285
      PHD_PHF7_G2E3_like; PHD finger found in PHD finger protein 7 (PHF7) and G2/M phase-specific E3 ubiquitin-protein ligase (G2E3)
      cd15669
      Location:14 → 127
      ePHD_PHF7_G2E3_like; Extended PHD finger found in PHD finger protein 7 (PHF7) and G2/M phase-specific E3 ubiquitin-protein ligase (G2E3)
      cl00077
      Location:365 → 681
      HECTc; HECT domain; C-terminal catalytic domain of a subclass of Ubiquitin-protein ligase (E3). It binds specific ubiquitin-conjugating enzymes (E2), accepts ubiquitin from E2, transfers ubiquitin to substrate lysine side chains, and transfers additional ...

    2. NM_001167963.1 → NP_001161435.1  G2/M phase-specific E3 ubiquitin-protein ligase isoform 2

      Status: VALIDATED

      Description
      Transcript Variant: This variant (2) contains an additional in-frame coding exon compared to variant 1, resulting in a longer isoform (2) containing a 23 aa protein segment not found in isoform 1.
      Source sequence(s)
      AA231622, AK033513, AK086526, AK129332, BY077085, CJ239277
      Consensus CDS
      CCDS49061.1
      UniProtKB/TrEMBL
      E9Q7C2
      Related
      ENSMUSP00000113270.2, ENSMUST00000119211.8
      Conserved Domains (4) summary
      smart00119
      Location:411 → 713
      HECTc; Domain Homologous to E6-AP Carboxyl Terminus with
      cd15496
      Location:232 → 285
      PHD_PHF7_G2E3_like; PHD finger found in PHD finger protein 7 (PHF7) and G2/M phase-specific E3 ubiquitin-protein ligase (G2E3)
      cd15669
      Location:14 → 127
      ePHD_PHF7_G2E3_like; Extended PHD finger found in PHD finger protein 7 (PHF7) and G2/M phase-specific E3 ubiquitin-protein ligase (G2E3)
      cl00077
      Location:388 → 704
      HECTc; HECT domain; C-terminal catalytic domain of a subclass of Ubiquitin-protein ligase (E3). It binds specific ubiquitin-conjugating enzymes (E2), accepts ubiquitin from E2, transfers ubiquitin to substrate lysine side chains, and transfers additional ...

    3. NM_001167964.1 → NP_001161436.1  G2/M phase-specific E3 ubiquitin-protein ligase isoform 3

      Status: VALIDATED

      Description
      Transcript Variant: This variant (3) is missing an internal coding exon and uses an alternate acceptor splice site at another coding exon compared to variant 1. However, it retains the reading frame and encodes a shorter isoform (3) lacking an internal protein segment compared to isoform 1.
      Source sequence(s)
      AA231622, AK031333, AK086526, AK129332, BY077085, CJ239277
      Consensus CDS
      CCDS49062.1
      UniProtKB/TrEMBL
      E9Q7E5
      Related
      ENSMUSP00000113191.2, ENSMUST00000121521.2
      Conserved Domains (3) summary
      cd15496
      Location:232 → 285
      PHD_PHF7_G2E3_like; PHD finger found in PHD finger protein 7 (PHF7) and G2/M phase-specific E3 ubiquitin-protein ligase (G2E3)
      cd15669
      Location:14 → 127
      ePHD_PHF7_G2E3_like; Extended PHD finger found in PHD finger protein 7 (PHF7) and G2/M phase-specific E3 ubiquitin-protein ligase (G2E3)
      cl00077
      Location:346 → 596
      HECTc; HECT domain; C-terminal catalytic domain of a subclass of Ubiquitin-protein ligase (E3). It binds specific ubiquitin-conjugating enzymes (E2), accepts ubiquitin from E2, transfers ubiquitin to substrate lysine side chains, and transfers additional ...

    RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCm39 C57BL/6J

    Genomic

    1. NC_000078.7 Reference GRCm39 C57BL/6J

      Range
      51395013..51423769
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)
    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q5RJY2.2 GenPept UniProtKB/Swiss-Prot:Q5RJY2

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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