Rnf20 ring finger protein 20 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Rnf20provided by MGI
Official Full Name: ring finger protein 20provided by MGI
Primary source: MGI:MGI:1925927
See related: Ensembl:ENSMUSG00000028309 AllianceGenome:MGI:1925927
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: mKIAA4116; 4833430L21Rik
Summary: Enables chromatin binding activity. Acts upstream of or within histone ubiquitination and positive regulation of histone methylation. Predicted to be located in nucleolus and nucleoplasm. Predicted to be part of HULC complex and ubiquitin ligase complex. Is expressed in several structures, including adrenal gland; central nervous system; eye; genitourinary system; and hemolymphoid system gland. Human ortholog(s) of this gene implicated in breast cancer. Orthologous to human RNF20 (ring finger protein 20). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Ubiquitous expression in CNS E11.5 (RPKM 22.3), CNS E14 (RPKM 15.3) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 4 B1; 4 26.69 cM

Exon count:: 23

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	4	NC_000070.7 (49632005..49656887)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	4	NC_000070.6 (49632005..49656887)

Chromosome 4 - NC_000070.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Impact of Ring Finger Protein 20 and Its Downstream Regulation on Renal Tubular Injury in a Unilateral Nephrectomy Mouse Model Fed a High-Fat Diet.
Title: Impact of Ring Finger Protein 20 and Its Downstream Regulation on Renal Tubular Injury in a Unilateral Nephrectomy Mouse Model Fed a High-Fat Diet.
Epigenetic regulator RNF20 underlies temporal hierarchy of gene expression to regulate postnatal cardiomyocyte polarization.
Title: Epigenetic regulator RNF20 underlies temporal hierarchy of gene expression to regulate postnatal cardiomyocyte polarization.
Adipocyte Rnf20 ablation increases the fast-twitch fibers of skeletal muscle via lysophosphatidylcholine 16:0.
Title: Adipocyte Rnf20 ablation increases the fast-twitch fibers of skeletal muscle via lysophosphatidylcholine 16:0.
RNF20 deletion causes inflammation in model of sepsis through the NLRP3 activation.
Title: RNF20 deletion causes inflammation in model of sepsis through the NLRP3 activation.
RNF20/RNF40 ameliorates streptozotocin-induced type 1 diabetes by activating vitamin D receptors in vivo.
Title: RNF20/RNF40 ameliorates streptozotocin-induced type 1 diabetes by activating vitamin D receptors in vivo.
Endothelial cells regulated by RNF20 orchestrate the proliferation and differentiation of neural precursor cells during embryonic development.
Title: Endothelial cells regulated by RNF20 orchestrate the proliferation and differentiation of neural precursor cells during embryonic development.
Rnf20 deficiency in adipocyte impairs adipose tissue development and thermogenesis.
Title: Rnf20 deficiency in adipocyte impairs adipose tissue development and thermogenesis.
Massively parallel in vivo CRISPR screening identifies RNF20/40 as epigenetic regulators of cardiomyocyte maturation.
Title: Massively parallel in vivo CRISPR screening identifies RNF20/40 as epigenetic regulators of cardiomyocyte maturation.
RNF20 promoted proteasomal degradation of nuclear corepressor 1 (NCoR1), which led to stimulation of the transcriptional activity of PPARgamma. Collectively, these data suggest that RNF20-NCoR1 is a novel axis in adipocyte biology through fine-tuning the transcriptional activity of PPARgamma.
Title: RNF20 Functions as a Transcriptional Coactivator for PPARγ by Promoting NCoR1 Degradation in Adipocytes.
These data suggest that Rnf20 functions at the Rfx3 locus regulating cilia motility and cardiac situs and identify H2Bub1 as an upstream transcriptional regulator controlling tissue-specific expression of cilia genes. Our findings mechanistically link the two functional gene ontologies that have been implicated in human CHD: chromatin remodeling and cilia function.
Title: Histone H2B monoubiquitination regulates heart development via epigenetic control of cilia motility.

Submit: New GeneRIF Correction See all GeneRIFs (21)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Gene trapped (1)
Targeted (5) 1 citation
Endonuclease-mediated (1)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

RH126660 (e-PCR)
- UniSTS:207617

AW540162 (e-PCR)
- UniSTS:178999

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables chromatin binding	IDA Inferred from Direct Assay more info	PubMed
enables histone H2B C-terminal K residue ubiquitin ligase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables histone H2B C-terminal K residue ubiquitin ligase activity	ISO Inferred from Sequence Orthology more info
enables histone binding	ISO Inferred from Sequence Orthology more info
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables mRNA 3'-UTR binding	ISO Inferred from Sequence Orthology more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables p53 binding	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables transcription coactivator activity	ISO Inferred from Sequence Orthology more info
enables transferase activity	IEA Inferred from Electronic Annotation more info
enables ubiquitin protein ligase activity	IBA Inferred from Biological aspect of Ancestor more info
enables ubiquitin protein ligase binding	ISO Inferred from Sequence Orthology more info
contributes_to ubiquitin-protein transferase activity	ISO Inferred from Sequence Orthology more info
enables ubiquitin-protein transferase activity	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within chromatin organization	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within chromatin remodeling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of cell migration	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of DNA-templated transcription	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info
involved_in protein polyubiquitination	ISO Inferred from Sequence Orthology more info
involved_in regulation of DNA-templated transcription	ISO Inferred from Sequence Orthology more info
involved_in ubiquitin-dependent protein catabolic process	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
part_of HULC complex	IBA Inferred from Biological aspect of Ancestor more info
part_of HULC complex	ISO Inferred from Sequence Orthology more info
located_in nucleolus	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	ISO Inferred from Sequence Orthology more info
part_of ubiquitin ligase complex	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: E3 ubiquitin-protein ligase BRE1A

Names: BRE1-A; RING-type E3 ubiquitin transferase BRE1A

NP_001156735.1

EC 2.3.2.27

NP_001343330.1

EC 2.3.2.27

NP_892044.1

EC 2.3.2.27

XP_006537616.1

EC 2.3.2.27

XP_011248208.1

EC 2.3.2.27

XP_030108941.1

EC 2.3.2.27

XP_036019473.1

EC 2.3.2.27

XP_036019474.1

EC 2.3.2.27

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001163263.1 → NP_001156735.1 E3 ubiquitin-protein ligase BRE1A isoform 1

See identical proteins and their annotated locations for NP_001156735.1

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longest transcript. Variants 1 and 2 encode the same protein.

Source sequence(s)

AK051278, AK139883, BF730643, BQ442966, CJ052397, CN531555, DV660388

Consensus CDS

CCDS18178.1

UniProtKB/Swiss-Prot

A2AIR3, Q3UT10, Q3V350, Q5DTM8, Q7TT11, Q8BKA8, Q8BKN8, Q8BUF7, Q8BVU4

Related

ENSMUSP00000128546.2, ENSMUST00000167496.8

Conserved Domains (3) summary

PTZ00121
Location:320 → 934

PTZ00121; MAEBL; Provisional

COG1196
Location:41 → 394

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]

cd16704
Location:918 → 963

RING-HC_RNF20_like; RING finger, HC subclass, found in RING finger protein RNF20, RNF40, and similar proteins
NM_001356401.1 → NP_001343330.1 E3 ubiquitin-protein ligase BRE1A isoform 2

Status: VALIDATED

Source sequence(s)

AK053783, AK139883, AL732521, BF730643, BQ442966, CN531555, DV660388

Conserved Domains (2) summary

PTZ00121
Location:75 → 689

PTZ00121; MAEBL; Provisional

cd16704
Location:673 → 718

RING-HC_RNF20_like; RING finger, HC subclass, found in RING finger protein RNF20, RNF40, and similar proteins
NM_182999.2 → NP_892044.1 E3 ubiquitin-protein ligase BRE1A isoform 1

See identical proteins and their annotated locations for NP_892044.1

Status: VALIDATED

Description

Transcript Variant: This variant (2) lacks a segment in the 5' UTR, compared to variant 1. Variants 1 and 2 encode the same protein.

Source sequence(s)

AK053783, AK139883, BF730643, BQ442966, CJ052397, CN531555, DV660388

Consensus CDS

CCDS18178.1

UniProtKB/Swiss-Prot

A2AIR3, Q3UT10, Q3V350, Q5DTM8, Q7TT11, Q8BKA8, Q8BKN8, Q8BUF7, Q8BVU4

Related

ENSMUSP00000029989.5, ENSMUST00000029989.11

Conserved Domains (3) summary

PTZ00121
Location:320 → 934

PTZ00121; MAEBL; Provisional

COG1196
Location:41 → 394

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]

cd16704
Location:918 → 963

RING-HC_RNF20_like; RING finger, HC subclass, found in RING finger protein RNF20, RNF40, and similar proteins

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000070.7 Reference GRCm39 C57BL/6J

Range

49632005..49656887

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006537553.4 → XP_006537616.1 E3 ubiquitin-protein ligase BRE1A isoform X1

See identical proteins and their annotated locations for XP_006537616.1

UniProtKB/Swiss-Prot

A2AIR3, Q3UT10, Q3V350, Q5DTM8, Q7TT11, Q8BKA8, Q8BKN8, Q8BUF7, Q8BVU4

Conserved Domains (3) summary

PTZ00121
Location:320 → 934

PTZ00121; MAEBL; Provisional

COG1196
Location:41 → 394

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]

cd16704
Location:918 → 963

RING-HC_RNF20_like; RING finger, HC subclass, found in RING finger protein RNF20, RNF40, and similar proteins
XM_011249906.4 → XP_011248208.1 E3 ubiquitin-protein ligase BRE1A isoform X1

See identical proteins and their annotated locations for XP_011248208.1

UniProtKB/Swiss-Prot

A2AIR3, Q3UT10, Q3V350, Q5DTM8, Q7TT11, Q8BKA8, Q8BKN8, Q8BUF7, Q8BVU4

Conserved Domains (3) summary

PTZ00121
Location:320 → 934

PTZ00121; MAEBL; Provisional

COG1196
Location:41 → 394

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]

cd16704
Location:918 → 963

RING-HC_RNF20_like; RING finger, HC subclass, found in RING finger protein RNF20, RNF40, and similar proteins
XM_036163580.1 → XP_036019473.1 E3 ubiquitin-protein ligase BRE1A isoform X2

Conserved Domains (2) summary

PTZ00121
Location:75 → 689

PTZ00121; MAEBL; Provisional

cd16704
Location:673 → 718

RING-HC_RNF20_like; RING finger, HC subclass, found in RING finger protein RNF20, RNF40, and similar proteins
XM_036163581.1 → XP_036019474.1 E3 ubiquitin-protein ligase BRE1A isoform X3

Conserved Domains (2) summary

PTZ00121
Location:51 → 665

PTZ00121; MAEBL; Provisional

cd16704
Location:649 → 694

RING-HC_RNF20_like; RING finger, HC subclass, found in RING finger protein RNF20, RNF40, and similar proteins
XM_030253081.2 → XP_030108941.1 E3 ubiquitin-protein ligase BRE1A isoform X2

Conserved Domains (2) summary

PTZ00121
Location:75 → 689

PTZ00121; MAEBL; Provisional

cd16704
Location:673 → 718

RING-HC_RNF20_like; RING finger, HC subclass, found in RING finger protein RNF20, RNF40, and similar proteins