Pde4b phosphodiesterase 4B, cAMP specific [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Pde4bprovided by MGI
Official Full Name: phosphodiesterase 4B, cAMP specificprovided by MGI
Primary source: MGI:MGI:99557
See related: Ensembl:ENSMUSG00000028525 AllianceGenome:MGI:99557
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Dpde4; dunce
Summary: Enables 3',5'-cyclic-AMP phosphodiesterase activity; gamma-tubulin binding activity; and transmembrane transporter binding activity. Involved in several processes, including cellular response to epinephrine stimulus; negative regulation of adenylate cyclase-activating adrenergic receptor signaling pathway involved in heart process; and regulation of muscle system process. Acts upstream of or within several processes, including cellular response to lipopolysaccharide; neutrophil chemotaxis; and neutrophil homeostasis. Located in several cellular components, including Z disc; centrosome; and synaptic vesicle. Part of gamma-tubulin complex and voltage-gated calcium channel complex. Is expressed in several structures, including alimentary system; ear; genitourinary system; nervous system; and respiratory system. Orthologous to human PDE4B (phosphodiesterase 4B). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Broad expression in cortex adult (RPKM 9.7), frontal lobe adult (RPKM 9.3) and 25 other tissues See more
Orthologs: human all
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Genomic context

Location:: 4 C6; 4 46.99 cM

Exon count:: 26

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	4	NC_000070.7 (101944529..102464459)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	4	NC_000070.6 (102087332..102607275)

Chromosome 4 - NC_000070.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

LncRNA GAS5 Knockdown Mitigates Hepatic Lipid Accumulation via Regulating MiR-26a-5p/PDE4B to Activate cAMP/CREB Pathway.
Title: LncRNA GAS5 Knockdown Mitigates Hepatic Lipid Accumulation via Regulating MiR-26a-5p/PDE4B to Activate cAMP/CREB Pathway.
Declines in PDE4B activity promote myopia progression through downregulation of scleral collagen expression.
Title: Declines in PDE4B activity promote myopia progression through downregulation of scleral collagen expression.
Phosphodiesterase 4B is required for NLRP3 inflammasome activation by positive feedback with Nrf2 in the early phase of LPS- induced acute lung injury.
Title: Phosphodiesterase 4B is required for NLRP3 inflammasome activation by positive feedback with Nrf2 in the early phase of LPS- induced acute lung injury.
Evidence for reprogramming of monocytes into reparative alveolar macrophages in vivo by targeting PDE4b.
Title: Evidence for reprogramming of monocytes into reparative alveolar macrophages in vivo by targeting PDE4b.
circPDE4B prevents articular cartilage degeneration and promotes repair by acting as a scaffold for RIC8A and MID1.
Title: circPDE4B prevents articular cartilage degeneration and promotes repair by acting as a scaffold for RIC8A and MID1.
Ablation of PDE4B protects from Pseudomonas aeruginosa-induced acute lung injury in mice by ameliorating the cytostorm and associated hypothermia.
Title: Ablation of PDE4B protects from Pseudomonas aeruginosa-induced acute lung injury in mice by ameliorating the cytostorm and associated hypothermia.
Selective Inhibition of PDE4B Reduces Binge Drinking in Two C57BL/6 Substrains.
Title: Selective Inhibition of PDE4B Reduces Binge Drinking in Two C57BL/6 Substrains.
these findings indicate that PDE4B plays an important role in the development of acute and chronic inflammatory response and consequent recovery following spinal cord injury
Title: Following spinal cord injury, PDE4B drives an acute, local inflammatory response and a chronic, systemic response exacerbated by gut dysbiosis and endotoxemia.
In vivo, the atropine-dependent prolongation of heart rate increase was blunted in PDE4D but not in wildtype or PDE4B knockout mice. The authors propose that inhibition of PDE4 by atropine accounts, at least in part, for the induction of tachycardia and the arrhythmogenic potency of this drug.
Title: Atropine augments cardiac contractility by inhibiting cAMP-specific phosphodiesterase type 4.
Study combined mutational analysis in the Apc mutated mice with published studies of frank colon cancer in patients to deduce that Pde4b has two strong biological functions. It negatively regulates colonic adenomagenesis in Apc mutated mice. In patients, PDE4B is most commonly inactivated by an epigenetic process.
Title: The conserved protective cyclic AMP-phosphodiesterase function PDE4B is expressed in the adenoma and adjacent normal colonic epithelium of mammals and silenced in colorectal cancer.

Submit: New GeneRIF Correction See all GeneRIFs (29)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (2) 1 citation
Chemically induced (ENU) (1) 1 citation
Endonuclease-mediated (3)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

BB248140 (e-PCR)
- UniSTS:207648

D4Mit155 (e-PCR), detects polymorphism
- UniSTS:116298

R74983 (e-PCR)
- UniSTS:125529

PMC25763P1 (e-PCR)
- UniSTS:272286

RH125139 (e-PCR)
- UniSTS:161961

Pde4b (e-PCR), detects polymorphism
- UniSTS:518826

22.MMHAP70FRH7.seq (e-PCR)
- UniSTS:123573

Pde4b (e-PCR), detects polymorphism
- UniSTS:507123

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables 3',5'-cyclic-AMP phosphodiesterase activity	IBA Inferred from Biological aspect of Ancestor more info
enables 3',5'-cyclic-AMP phosphodiesterase activity	IDA Inferred from Direct Assay more info	PubMed
enables 3',5'-cyclic-AMP phosphodiesterase activity	ISO Inferred from Sequence Orthology more info
enables 3',5'-cyclic-AMP phosphodiesterase activity	ISS Inferred from Sequence or Structural Similarity more info	PubMed
enables 3',5'-cyclic-GMP phosphodiesterase activity	IBA Inferred from Biological aspect of Ancestor more info
enables cAMP binding	ISO Inferred from Sequence Orthology more info
enables calcium channel regulator activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables gamma-tubulin binding	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables transmembrane transporter binding	IBA Inferred from Biological aspect of Ancestor more info
enables transmembrane transporter binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in T cell receptor signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in cAMP-mediated signaling	IBA Inferred from Biological aspect of Ancestor more info
involved_in cellular response to epinephrine stimulus	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within cellular response to lipopolysaccharide	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within cellular response to xenobiotic stimulus	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within leukocyte migration	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of adenylate cyclase-activating adrenergic receptor signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of relaxation of cardiac muscle	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within neutrophil chemotaxis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within neutrophil homeostasis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of interleukin-2 production	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of type II interferon production	ISO Inferred from Sequence Orthology more info
involved_in regulation of calcium ion transmembrane transport via high voltage-gated calcium channel	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of cardiac muscle cell contraction	IMP Inferred from Mutant Phenotype more info	PubMed
NOT acts_upstream_of_or_within smooth muscle contraction	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in Z disc	IMP Inferred from Mutant Phenotype more info	PubMed
located_in cell periphery	IDA Inferred from Direct Assay more info	PubMed
located_in centrosome	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
located_in dendritic spine	IDA Inferred from Direct Assay more info	PubMed
located_in excitatory synapse	IDA Inferred from Direct Assay more info	PubMed
part_of gamma-tubulin complex	IDA Inferred from Direct Assay more info	PubMed
located_in membrane	IDA Inferred from Direct Assay more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
is_active_in perinuclear region of cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in perinuclear region of cytoplasm	ISO Inferred from Sequence Orthology more info
located_in postsynaptic density	IDA Inferred from Direct Assay more info	PubMed
located_in synaptic vesicle	IDA Inferred from Direct Assay more info	PubMed
part_of voltage-gated calcium channel complex	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: 3',5'-cyclic-AMP phosphodiesterase 4B

NP_001171451.1

EC 3.1.4.17

NP_001171452.1

EC 3.1.4.17

NP_001171453.1

EC 3.1.4.17

NP_001171454.1

EC 3.1.4.17

NP_001361709.1

EC 3.1.4.17

NP_001391715.1

EC 3.1.4.17

NP_001391716.1

EC 3.1.4.17

NP_001391717.1

EC 3.1.4.17

NP_001391718.1

EC 3.1.4.17

NP_001391720.1

EC 3.1.4.17

NP_001391722.1

EC 3.1.4.17

NP_001391723.1

EC 3.1.4.17

NP_001391725.1

EC 3.1.4.17

NP_001391733.1

EC 3.1.4.17

NP_001391765.1

EC 3.1.4.17

NP_062814.2

EC 3.1.4.17

XP_006502919.1

EC 3.1.4.17

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001177980.2 → NP_001171451.1 3',5'-cyclic-AMP phosphodiesterase 4B isoform 2

Status: VALIDATED

Description

Transcript Variant: This variant (2) lacks several 5' exons but has an alternate 5' segment, as compared to variant 1. The resulting isoform (2) is shorter and has a different N-terminus, as compared to isoform 1.

Source sequence(s)

AL732551, AL772358

Consensus CDS

CCDS51243.1

UniProtKB/TrEMBL

B1AWD0, Q91VY2

Related

ENSMUSP00000095562.3, ENSMUST00000097950.9

Conserved Domains (1) summary

pfam00233
Location:233 → 474

PDEase_I; 3'5'-cyclic nucleotide phosphodiesterase
NM_001177981.1 → NP_001171452.1 3',5'-cyclic-AMP phosphodiesterase 4B isoform 3

See identical proteins and their annotated locations for NP_001171452.1

Status: VALIDATED

Description

Transcript Variant: This variant (3) lacks several 5' exons but has an alternate 5' exon, as compared to variant 1. The resulting isoform (3) is shorter and has a different N-terminus, as compared to isoform 1.

Source sequence(s)

AL732551, BB654856, BC071259

Consensus CDS

CCDS51244.1

UniProtKB/TrEMBL

Q6IQY6, Q91VY2

Related

ENSMUSP00000095561.5, ENSMUST00000097949.11

Conserved Domains (1) summary

pfam00233
Location:172 → 413

PDEase_I; 3'5'-cyclic nucleotide phosphodiesterase
NM_001177982.1 → NP_001171453.1 3',5'-cyclic-AMP phosphodiesterase 4B isoform 4

See identical proteins and their annotated locations for NP_001171453.1

Status: VALIDATED

Description

Transcript Variant: This variant (4) lacks several 5' exons but has an alternate 5' exon, as compared to variant 1. The resulting isoform (4) has a much shorter N-terminus, as compared to isoform 1.

Source sequence(s)

AK161341, AL732551

Consensus CDS

CCDS51245.1

UniProtKB/TrEMBL

Q3TTI9, Q91VY2

Related

ENSMUSP00000030251.7, ENSMUST00000030251.13

Conserved Domains (1) summary

pfam00233
Location:153 → 394

PDEase_I; 3'5'-cyclic nucleotide phosphodiesterase
NM_001177983.2 → NP_001171454.1 3',5'-cyclic-AMP phosphodiesterase 4B isoform 5

See identical proteins and their annotated locations for NP_001171454.1

Status: VALIDATED

Description

Transcript Variant: This variant (5) lacks several 3' exons but has an alternate 3' exon, as compared to variant 1. The resulting isoform (5) is shorter and has a different C-terminus, as compared to isoform 1.

Source sequence(s)

AL772293, AL772358

Consensus CDS

CCDS57289.1

UniProtKB/TrEMBL

Q80VK8

Related

ENSMUSP00000102520.3, ENSMUST00000106907.9
NM_001374780.2 → NP_001361709.1 3',5'-cyclic-AMP phosphodiesterase 4B isoform 6

Status: VALIDATED

Source sequence(s)

AL732551, AL772358, AL807794

Consensus CDS

CCDS89787.1

UniProtKB/TrEMBL

B1AWC8, Q9QXI7

Related

ENSMUSP00000102517.3, ENSMUST00000106904.9

Conserved Domains (2) summary

pfam00233
Location:328 → 569

PDEase_I; 3'5'-cyclic nucleotide phosphodiesterase

pfam18100
Location:89 → 204

PDE4_UCR; Phosphodiesterase 4 upstream conserved regions (UCR)
NM_001404786.1 → NP_001391715.1 3',5'-cyclic-AMP phosphodiesterase 4B isoform 7

Status: VALIDATED

Source sequence(s)

AL732551, AL772293, AL772358

UniProtKB/TrEMBL

B1AWC9

Related

ENSMUSP00000102524.2, ENSMUST00000106911.8
NM_001404787.1 → NP_001391716.1 3',5'-cyclic-AMP phosphodiesterase 4B isoform 8

Status: VALIDATED

Source sequence(s)

AL772293, AL772358
NM_001404788.1 → NP_001391717.1 3',5'-cyclic-AMP phosphodiesterase 4B isoform 9

Status: VALIDATED

Source sequence(s)

AL772293, AL772358
NM_001404789.1 → NP_001391718.1 3',5'-cyclic-AMP phosphodiesterase 4B isoform 6

Status: VALIDATED

Source sequence(s)

AL732551, AL772293, AL772358, AL807794

UniProtKB/TrEMBL

B1AWC8
NM_001404791.1 → NP_001391720.1 3',5'-cyclic-AMP phosphodiesterase 4B isoform 10

Status: VALIDATED

Source sequence(s)

AL732551, AL772293, AL772358
NM_001404793.1 → NP_001391722.1 3',5'-cyclic-AMP phosphodiesterase 4B isoform 11

Status: VALIDATED

Source sequence(s)

AL732551, AL772358
NM_001404794.1 → NP_001391723.1 3',5'-cyclic-AMP phosphodiesterase 4B isoform 12

Status: VALIDATED

Source sequence(s)

AL732551, AL772358
NM_001404796.1 → NP_001391725.1 3',5'-cyclic-AMP phosphodiesterase 4B isoform 13

Status: VALIDATED

Source sequence(s)

AL732551, AL772358
NM_001404804.1 → NP_001391733.1 3',5'-cyclic-AMP phosphodiesterase 4B isoform 14

Status: VALIDATED

Source sequence(s)

AL732551, AL772358
NM_001404836.1 → NP_001391765.1 3',5'-cyclic-AMP phosphodiesterase 4B isoform 15

Status: VALIDATED

Source sequence(s)

AL732551, AL772358
NM_019840.3 → NP_062814.2 3',5'-cyclic-AMP phosphodiesterase 4B isoform 1

See identical proteins and their annotated locations for NP_062814.2

Status: VALIDATED

Description

Transcript Variant: This variant (1) encodes the longest isoform (1).

Source sequence(s)

AL732551, AL772293, AL772358

Consensus CDS

CCDS18403.1

UniProtKB/TrEMBL

Q8VBU5, Q9QXI7

Related

ENSMUSP00000102521.3, ENSMUST00000106908.9

Conserved Domains (1) summary

pfam00233
Location:390 → 631

PDEase_I; 3'5'-cyclic nucleotide phosphodiesterase

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000070.7 Reference GRCm39 C57BL/6J

Range

101944529..102464459

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006502856.2 → XP_006502919.1 3',5'-cyclic-AMP phosphodiesterase 4B isoform X2

UniProtKB/TrEMBL

Q9QXI7

Conserved Domains (2) summary

pfam00233
Location:344 → 585

PDEase_I; 3'5'-cyclic nucleotide phosphodiesterase

pfam18100
Location:105 → 220

PDE4_UCR; Phosphodiesterase 4 upstream conserved regions (UCR)