Ube2b ubiquitin-conjugating enzyme E2B [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Ube2bprovided by MGI
Official Full Name: ubiquitin-conjugating enzyme E2Bprovided by MGI
Primary source: MGI:MGI:102944
See related: Ensembl:ENSMUSG00000020390 AllianceGenome:MGI:102944
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: HR6B; E214K; Rad6b; mHR6B; E2-14k
Summary: Enables ubiquitin conjugating enzyme activity and ubiquitin protein ligase binding activity. Involved in positive regulation of canonical Wnt signaling pathway. Acts upstream of or within several processes, including chromosome organization; positive regulation of reciprocal meiotic recombination; and sperm axoneme assembly. Located in XY body and chromatin. Is expressed in several structures, including alimentary system; brain; cardiovascular system; genitourinary system; and immune system. Orthologous to human UBE2B (ubiquitin conjugating enzyme E2 B). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Ubiquitous expression in bladder adult (RPKM 40.4), heart adult (RPKM 37.3) and 27 other tissues See more
Orthologs: human all
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Genomic context

Location:: 11 B1.3; 11 31.82 cM

Exon count:: 10

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	11	NC_000077.7 (51875965..51894972, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	11	NC_000077.6 (51985138..52004145, complement)

Chromosome 11 - NC_000077.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The Role of RAD6B and PEDF in Retinal Degeneration.
Title: The Role of RAD6B and PEDF in Retinal Degeneration.
Loss of RAD6B induces degeneration of the cochlea in mice.
Title: Loss of RAD6B induces degeneration of the cochlea in mice.
Results reveal that RAD6B and RNF8 contribute to nucleosome removal during spermiogenesis.
Title: Function of RAD6B and RNF8 in spermatogenesis.
RNF168, in complex with RAD6A or RAD6B, is activated in the DNA-damage-induced protein ubiquitination cascade.
Title: RNF168 forms a functional complex with RAD6 during the DNA damage response.
These results suggest that Ube2b, as a direct androgen receptor transcriptional target in Sertoli cells, mediates the function of androgen recpetor in spermatogenesis by promoting H2A ubiquitylation.
Title: Identification of Ube2b as a novel target of androgen receptor in mouse sertoli cells.
HR6B represses major satellite repeat transcription in spermatocytes, and functions in the maintenance of X chromosome silencing in spermatocytes and spermatids.
Title: The ubiquitin-conjugating enzyme HR6B is required for maintenance of X chromosome silencing in mouse spermatocytes and spermatids.
HR6B exerts control over different histone modifications in spermatocytes and spermatids, and that this function contributes to the postmeiotic maintenance of X chromosome silencing.
Title: Increased phosphorylation and dimethylation of XY body histones in the Hr6b-knockout mouse is associated with derepression of the X chromosome.
Ube2b deficient mice are shown to present male infertility and sperm head shape anomalies.
Title: Spermatid nuclear and sperm periaxonemal anomalies in the mouse Ube2b null mutant.
The present findings reveal a specific requirement for the ubiquitin-conjugating activity of HR6B in relation to dynamic aspects of the synaptonemal complex and meiotic recombination in spermatocytes.
Title: Loss of HR6B ubiquitin-conjugating activity results in damaged synaptonemal complex structure and increased crossing-over frequency during the male meiotic prophase.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (4) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables nucleotide binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables transferase activity	IEA Inferred from Electronic Annotation more info
enables ubiquitin conjugating enzyme activity	IBA Inferred from Biological aspect of Ancestor more info
enables ubiquitin conjugating enzyme activity	IGI Inferred from Genetic Interaction more info	PubMed
enables ubiquitin protein ligase binding	IDA Inferred from Direct Assay more info	PubMed
enables ubiquitin protein ligase binding	ISO Inferred from Sequence Orthology more info
enables ubiquitin-protein transferase activity	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in DNA damage response	ISO Inferred from Sequence Orthology more info
involved_in DNA repair	IBA Inferred from Biological aspect of Ancestor more info
involved_in DNA repair	ISO Inferred from Sequence Orthology more info
acts_upstream_of apoptotic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within chiasma assembly	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within chromatin organization	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of ectopic germ cell programmed cell death	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within in utero embryonic development	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within leptotene	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within meiotic telomere clustering	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within negative regulation of apoptotic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of cAMP-mediated signaling	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within negative regulation of developmental process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within negative regulation of post-translational protein modification	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within negative regulation of reproductive process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of canonical Wnt signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within positive regulation of reciprocal meiotic recombination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in postreplication repair	ISO Inferred from Sequence Orthology more info
involved_in proteasome-mediated ubiquitin-dependent protein catabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in proteasome-mediated ubiquitin-dependent protein catabolic process	ISO Inferred from Sequence Orthology more info
involved_in protein K11-linked ubiquitination	ISO Inferred from Sequence Orthology more info
involved_in protein K48-linked ubiquitination	ISO Inferred from Sequence Orthology more info
involved_in protein K63-linked ubiquitination	ISO Inferred from Sequence Orthology more info
involved_in protein autoubiquitination	ISO Inferred from Sequence Orthology more info
involved_in protein polyubiquitination	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein stabilization	ISO Inferred from Sequence Orthology more info
involved_in protein ubiquitination	ISO Inferred from Sequence Orthology more info
involved_in response to UV	ISO Inferred from Sequence Orthology more info
involved_in response to xenobiotic stimulus	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within sperm axoneme assembly	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within spermatogenesis	TAS Traceable Author Statement more info	PubMed
acts_upstream_of_or_within synaptonemal complex organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in ubiquitin-dependent protein catabolic process	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
part_of HULC complex	IBA Inferred from Biological aspect of Ancestor more info
part_of HULC complex	ISO Inferred from Sequence Orthology more info
located_in XY body	IDA Inferred from Direct Assay more info	PubMed
part_of chromatin	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in nucleus	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	IEA Inferred from Electronic Annotation more info
located_in replication fork	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: ubiquitin-conjugating enzyme E2 B

Names: E2 ubiquitin-conjugating enzyme B; RAD6 homolog B; ubiquitin carrier protein B; ubiquitin-conjugating enzyme E2B, RAD6 homology; ubiquitin-protein ligase B

NP_001349614.1

EC 2.3.2.23

NP_001349615.1

EC 2.3.2.23

NP_033484.3

EC 2.3.2.23

XP_011247269.1

EC 2.3.2.23

XP_030101785.1

EC 2.3.2.23

XP_030101786.1

EC 2.3.2.23

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001362685.1 → NP_001349614.1 ubiquitin-conjugating enzyme E2 B isoform 1

Status: VALIDATED

Source sequence(s)

AL669920, CF951907

Consensus CDS

CCDS24664.1

UniProtKB/Swiss-Prot

P63147, Q3UGS2

UniProtKB/TrEMBL

A2RSE4, Q3TUV9

Related

ENSMUSP00000104714.2, ENSMUST00000109086.8

Conserved Domains (1) summary

pfam00179
Location:8 → 145

UQ_con; Ubiquitin-conjugating enzyme
NM_001362686.1 → NP_001349615.1 ubiquitin-conjugating enzyme E2 B isoform 2

Status: VALIDATED

Source sequence(s)

AL669920

Conserved Domains (1) summary

pfam00179
Location:8 → 115

UQ_con; Ubiquitin-conjugating enzyme
NM_009458.5 → NP_033484.3 ubiquitin-conjugating enzyme E2 B isoform 1

See identical proteins and their annotated locations for NP_033484.3

Status: VALIDATED

Source sequence(s)

AK147785, AK169229, AL669920, BE944184

Consensus CDS

CCDS24664.1

UniProtKB/Swiss-Prot

P63147, Q3UGS2

UniProtKB/TrEMBL

A2RSE4, Q3TUV9

Related

ENSMUSP00000020657.7, ENSMUST00000020657.13

Conserved Domains (1) summary

pfam00179
Location:8 → 145

UQ_con; Ubiquitin-conjugating enzyme

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000077.7 Reference GRCm39 C57BL/6J

Range

51875965..51894972 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_030245925.1 → XP_030101785.1 ubiquitin-conjugating enzyme E2 B isoform X1

UniProtKB/Swiss-Prot

P63147, Q3UGS2

UniProtKB/TrEMBL

A2RSE4, Q3TUV9

Conserved Domains (1) summary

pfam00179
Location:8 → 145

UQ_con; Ubiquitin-conjugating enzyme
XM_011248967.2 → XP_011247269.1 ubiquitin-conjugating enzyme E2 B isoform X1

See identical proteins and their annotated locations for XP_011247269.1

UniProtKB/Swiss-Prot

P63147, Q3UGS2

UniProtKB/TrEMBL

A2RSE4, Q3TUV9

Conserved Domains (1) summary

pfam00179
Location:8 → 145

UQ_con; Ubiquitin-conjugating enzyme
XM_030245926.1 → XP_030101786.1 ubiquitin-conjugating enzyme E2 B isoform X2

Conserved Domains (1) summary

pfam00179
Location:8 → 115

UQ_con; Ubiquitin-conjugating enzyme