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    Orc4 origin recognition complex, subunit 4 [ Mus musculus (house mouse) ]

    Gene ID: 26428, updated on 11-Apr-2024

    Summary

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    Official Symbol
    Orc4provided by MGI
    Official Full Name
    origin recognition complex, subunit 4provided by MGI
    Primary source
    MGI:MGI:1347043
    See related
    Ensembl:ENSMUSG00000026761 AllianceGenome:MGI:1347043
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Mus musculus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
    Also known as
    Orc4P; Orc4l; mMmORC4
    Summary
    Predicted to enable DNA replication origin binding activity and nucleotide binding activity. Predicted to be involved in DNA replication initiation. Predicted to act upstream of or within DNA replication. Predicted to be located in cytosol; nucleolus; and nucleoplasm. Predicted to be part of nuclear origin of replication recognition complex. Predicted to colocalize with chromosome, telomeric region. Is expressed in brain; embryo; and pancreas epithelium. Human ortholog(s) of this gene implicated in Meier-Gorlin syndrome 2. Orthologous to human ORC4 (origin recognition complex subunit 4). [provided by Alliance of Genome Resources, Apr 2022]
    Expression
    Broad expression in CNS E18 (RPKM 9.0), CNS E14 (RPKM 7.9) and 25 other tissues See more
    Orthologs
    human all
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    Genomic context

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    Location:
    2 C1.1; 2 28.42 cM
    Exon count:
    17
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCm39 (GCF_000001635.27) 2 NC_000068.8 (48792836..48840287, complement)
    108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 2 NC_000068.7 (48902824..48952902, complement)

    Chromosome 2 - NC_000068.8Genomic Context describing neighboring genes Neighboring gene predicted gene, 31324 Neighboring gene glyceraldehyde-3-phosphate dehydrogenase pseudogene Neighboring gene activin receptor IIA Neighboring gene CapStarr-seq enhancer MGSCv37_chr2:48804892-48805001 Neighboring gene methyl-CpG binding domain protein 5 Neighboring gene predicted gene 13510 Neighboring gene adaptor-related protein complex 2, mu 1 subunit, pseudogene

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: Mouse ENCODE transcriptome data
    • Description: RNA profiling data sets generated by the Mouse ENCODE project.
    • BioProject: PRJNA66167
    • Publication: PMID 25409824
    • Analysis date: n/a

    Bibliography

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    Related articles in PubMed

    1. The role of ORC4 in enucleation of Murine Erythroleukemia (MEL) cells is similar to that in oocyte polar body extrusion. Nguyen H, et al. Syst Biol Reprod Med, 2020 Dec. PMID 32972244, Free PMC Article
    2. Higher Order Oligomerization of the Licensing ORC4 Protein Is Required for Polar Body Extrusion in Murine Meiosis. Nguyen H, et al. J Cell Biochem, 2017 Sep. PMID 28230328, Free PMC Article
    3. ORC4 surrounds extruded chromatin in female meiosis. Nguyen H, et al. J Cell Biochem, 2015 May. PMID 25502171, Free PMC Article
    4. Deletion of Orc4 during oogenesis severely reduces polar body extrusion and blocks zygotic DNA replication†. Nguyen H, et al. Biol Reprod, 2022 Apr 26. PMID 34977916, Free PMC Article
    5. Disruption of Mbd5 in mice causes neuronal functional deficits and neurobehavioral abnormalities consistent with 2q23.1 microdeletion syndrome. Camarena V, et al. EMBO Mol Med, 2014 Aug. PMID 25001218, Free PMC Article

    See all (26) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. The role of ORC4 in enucleation of Murine Erythroleukemia (MEL) cells is similar to that in oocyte polar body extrusion.
      Title: The role of ORC4 in enucleation of Murine Erythroleukemia (MEL) cells is similar to that in oocyte polar body extrusion.
    2. Together, these data demonstrate that ORC4 oligomerization is required to form the ORC4 cage and that it is required for polar body extrusion.
      Title: Higher Order Oligomerization of the Licensing ORC4 Protein Is Required for Polar Body Extrusion in Murine Meiosis.
    3. ORC4 surrounds the chromatin that will be extruded in the polar body in both female meiotic divisions, then makes a transition from the cytoplasm to the chromosomes at zygotic anaphase, suggesting multiple roles for this replication licensing protein.
      Title: ORC4 surrounds extruded chromatin in female meiosis.
    Submit: New GeneRIF Correction

    Variation

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    Alleles

    Alleles of this type are documented at Mouse Genome Informatics  (MGI)
    • Gene trapped (1) 
    • Targeted (3)  1 citation
    • Endonuclease-mediated (1) 

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Markers

    Homology

    Gene Ontology Provided by MGI

    Function Evidence Code Pubs
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ATP hydrolysis activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA replication origin binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables DNA replication origin binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables nucleotide binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    Process Evidence Code Pubs
    acts_upstream_of_or_within DNA replication IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within DNA replication TAS
    Traceable Author Statement
    more info
    		 PubMed 
    involved_in DNA replication initiation IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in DNA replication initiation ISO
    Inferred from Sequence Orthology
    more info
    		  
    acts_upstream_of_or_within cellular process involved in reproduction in multicellular organism IC
    Inferred by Curator
    more info
    		 PubMed 
    acts_upstream_of_or_within meiotic cell cycle process IC
    Inferred by Curator
    more info
    		 PubMed 
    acts_upstream_of_or_within polar body extrusion after meiotic divisions IC
    Inferred by Curator
    more info
    		 PubMed 
    involved_in polar body extrusion after meiotic divisions IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in protein polymerization IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Component Evidence Code Pubs
    colocalizes_with chromosome, telomeric region ISO
    Inferred from Sequence Orthology
    more info
    		  
    is_active_in cytoplasm IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cytosol ISO
    Inferred from Sequence Orthology
    more info
    		  
    part_of nuclear origin of replication recognition complex IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of nuclear origin of replication recognition complex ISO
    Inferred from Sequence Orthology
    more info
    		  
    part_of nuclear origin of replication recognition complex NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    located_in nucleolus ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in nucleoplasm ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in nucleus ISO
    Inferred from Sequence Orthology
    more info
    		  
    part_of origin recognition complex ISO
    Inferred from Sequence Orthology
    more info
    		  

    General protein information

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    Preferred Names
    origin recognition complex subunit 4

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001177313.2 → NP_001170784.1  origin recognition complex subunit 4 isoform 2

      See identical proteins and their annotated locations for NP_001170784.1

      Status: VALIDATED

      Description
      Transcript Variant: This variant (2) lacks multiple 3' exons, but has an alternate 3' exon, as compared to variant 1. The resulting isoform (2) is shorter and has a different C-terminus, as compared to isoform 1. Variants 2, 11, 12, and 13 all encode the same isoform (2).
      Source sequence(s)
      AL732317, AL845521
      Consensus CDS
      CCDS50582.1
      UniProtKB/TrEMBL
      Q9CZF7
      Related
      ENSMUSP00000088497.4, ENSMUST00000090976.10
      Conserved Domains (2) summary
      PRK00411
      Location:46 → 130
      cdc6; ORC1-type DNA replication protein
      pfam13191
      Location:35 → 82
      AAA_16; AAA ATPase domain

    2. NM_001355296.2 → NP_001342225.1  origin recognition complex subunit 4 isoform 1

      Status: VALIDATED

      Description
      Transcript Variant: This variant (4) differs in the 5' UTR compared to variant 1. Variants 1, 4, 7, and 8 all encode the same isoform (1).
      Source sequence(s)
      AL732317, AL845521
      Consensus CDS
      CCDS16022.1
      UniProtKB/Swiss-Prot
      O88708, Q91V62, Q9QYX1
      UniProtKB/TrEMBL
      Q8BN98, Q8C693
      Conserved Domains (2) summary
      COG1474
      Location:41 → 223
      CDC6; Cdc6-related protein, AAA superfamily ATPase [Replication, recombination and repair]
      pfam14629
      Location:225 → 412
      ORC4_C; Origin recognition complex (ORC) subunit 4 C-terminus

    3. NM_001421264.1 → NP_001408193.1  origin recognition complex subunit 4 isoform 1

      Status: VALIDATED

      Source sequence(s)
      AL732317, AL845521
      UniProtKB/Swiss-Prot
      O88708, Q91V62, Q9QYX1

    4. NM_001421265.1 → NP_001408194.1  origin recognition complex subunit 4 isoform 1

      Status: VALIDATED

      Source sequence(s)
      AL732317, AL845521
      UniProtKB/Swiss-Prot
      O88708, Q91V62, Q9QYX1

    5. NM_001421266.1 → NP_001408195.1  origin recognition complex subunit 4 isoform 3

      Status: VALIDATED

      Source sequence(s)
      AL732317, AL845521

    6. NM_001421267.1 → NP_001408196.1  origin recognition complex subunit 4 isoform 4

      Status: VALIDATED

      Source sequence(s)
      AL732317, AL845521

    7. NM_001421268.1 → NP_001408197.1  origin recognition complex subunit 4 isoform 2

      Status: VALIDATED

      Source sequence(s)
      AL732317, AL845521
      UniProtKB/TrEMBL
      Q9CZF7

    8. NM_001421269.1 → NP_001408198.1  origin recognition complex subunit 4 isoform 2

      Status: VALIDATED

      Source sequence(s)
      AL732317, AL845521
      UniProtKB/TrEMBL
      Q9CZF7

    9. NM_001421270.1 → NP_001408199.1  origin recognition complex subunit 4 isoform 2

      Status: VALIDATED

      Source sequence(s)
      AL732317, AL845521
      UniProtKB/TrEMBL
      Q9CZF7

    10. NM_011958.5 → NP_036088.3  origin recognition complex subunit 4 isoform 1

      See identical proteins and their annotated locations for NP_036088.3

      Status: VALIDATED

      Description
      Transcript Variant: This variant (1) encodes the longest isoform (1). Variants 1, 4, 7, and 8 all encode the same isoform (1).
      Source sequence(s)
      AL732317, AL845521
      Consensus CDS
      CCDS16022.1
      UniProtKB/Swiss-Prot
      O88708, Q91V62, Q9QYX1
      UniProtKB/TrEMBL
      Q8BN98, Q8C693
      Related
      ENSMUSP00000028098.5, ENSMUST00000028098.11
      Conserved Domains (2) summary
      COG1474
      Location:41 → 223
      CDC6; Cdc6-related protein, AAA superfamily ATPase [Replication, recombination and repair]
      pfam14629
      Location:225 → 412
      ORC4_C; Origin recognition complex (ORC) subunit 4 C-terminus

    RNA

    1. NR_033442.3 RNA Sequence

      Status: VALIDATED

      Description
      Transcript Variant: This variant (3) lacks an internal segment in the 3' CDS, as compared to variant 1. The transcript is a nonsense-mediated mRNA decay candidate and is probably not making a protein.
      Source sequence(s)
      AL732317, AL845521

    2. NR_149355.2 RNA Sequence

      Status: VALIDATED

      Source sequence(s)
      AL732317, AL845521

    3. NR_149356.2 RNA Sequence

      Status: VALIDATED

      Source sequence(s)
      AL732317, AL845521

    4. NR_185286.1 RNA Sequence

      Status: VALIDATED

      Source sequence(s)
      AL732317, AL845521

    5. NR_185287.1 RNA Sequence

      Status: VALIDATED

      Source sequence(s)
      AL732317, AL845521

    6. NR_185288.1 RNA Sequence

      Status: VALIDATED

      Source sequence(s)
      AL732317, AL845521

    7. NR_185289.1 RNA Sequence

      Status: VALIDATED

      Source sequence(s)
      AL732317, AL845521

    RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCm39 C57BL/6J

    Genomic

    1. NC_000068.8 Reference GRCm39 C57BL/6J

      Range
      48792836..48840287 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_006498066.4 → XP_006498129.1  origin recognition complex subunit 4 isoform X2

      UniProtKB/TrEMBL
      Q8BN98, Q8C693
      Conserved Domains (2) summary
      COG1474
      Location:41 → 190
      CDC6; Cdc6-related protein, AAA superfamily ATPase [Replication, recombination and repair]
      pfam14629
      Location:195 → 354
      ORC4_C; Origin recognition complex (ORC) subunit 4 C-terminus

    2. XM_030251581.1 → XP_030107441.1  origin recognition complex subunit 4 isoform X2

      UniProtKB/TrEMBL
      Q8BN98, Q8C693
      Conserved Domains (2) summary
      COG1474
      Location:41 → 190
      CDC6; Cdc6-related protein, AAA superfamily ATPase [Replication, recombination and repair]
      pfam14629
      Location:195 → 354
      ORC4_C; Origin recognition complex (ORC) subunit 4 C-terminus
    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    O88708.2 GenPept UniProtKB/Swiss-Prot:O88708

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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