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    Mgat4a mannoside acetylglucosaminyltransferase 4, isoenzyme A [ Mus musculus (house mouse) ]

    Gene ID: 269181, updated on 5-Mar-2024

    Summary

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    Official Symbol
    Mgat4aprovided by MGI
    Official Full Name
    mannoside acetylglucosaminyltransferase 4, isoenzyme Aprovided by MGI
    Primary source
    MGI:MGI:2662992
    See related
    Ensembl:ENSMUSG00000026110 AllianceGenome:MGI:2662992
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Mus musculus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
    Also known as
    GnT-IVa; glcNAc-T-IVa; 9530018I07Rik
    Summary
    Enables acetylglucosaminyltransferase activity. Acts upstream of or within protein N-linked glycosylation. Predicted to be located in Golgi apparatus; extracellular region; and membrane. Predicted to be integral component of membrane. Predicted to be active in Golgi stack; endoplasmic reticulum; and endoplasmic reticulum-Golgi intermediate compartment. Is expressed in several structures, including alimentary system; genitourinary system; nervous system; nose; and respiratory system. Orthologous to human MGAT4A (alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A). [provided by Alliance of Genome Resources, Apr 2022]
    Expression
    Broad expression in large intestine adult (RPKM 27.4), colon adult (RPKM 23.5) and 20 other tissues See more
    Orthologs
    human all
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    Genomic context

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    See Mgat4a in Genome Data Viewer
    Location:
    1 B; 1 15.46 cM
    Exon count:
    19
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCm39 (GCF_000001635.27) 1 NC_000067.7 (37478421..37580599, complement)
    108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 1 NC_000067.6 (37439340..37541060, complement)

    Chromosome 1 - NC_000067.7Genomic Context describing neighboring genes Neighboring gene cytochrome C oxidase assembly factor 5 Neighboring gene unc-50 homolog Neighboring gene CapStarr-seq enhancer MGSCv37_chr1:37528102-37528303 Neighboring gene STARR-positive B cell enhancer ABC_E1540 Neighboring gene G1 to S phase transition pseudogene Neighboring gene STARR-positive B cell enhancer ABC_E1541 Neighboring gene STARR-seq mESC enhancer starr_00677 Neighboring gene predicted gene, 39627 Neighboring gene STARR-positive B cell enhancer ABC_E5748 Neighboring gene CapStarr-seq enhancer MGSCv37_chr1:37642841-37643028 Neighboring gene CapStarr-seq enhancer MGSCv37_chr1:37643098-37643207 Neighboring gene RIKEN cDNA 4930556I23 gene Neighboring gene CapStarr-seq enhancer MGSCv37_chr1:37671888-37672102 Neighboring gene capping protein inhibiting regulator of actin like Neighboring gene STARR-seq mESC enhancer starr_00679 Neighboring gene RIKEN cDNA 4930470B04 gene Neighboring gene STARR-seq mESC enhancer starr_00681

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: Mouse ENCODE transcriptome data
    • Description: RNA profiling data sets generated by the Mouse ENCODE project.
    • BioProject: PRJNA66167
    • Publication: PMID 25409824
    • Analysis date: n/a

    Bibliography

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    Related articles in PubMed

    1. Discovery of a lectin domain that regulates enzyme activity in mouse N-acetylglucosaminyltransferase-IVa (MGAT4A). Nagae M, et al. Commun Biol, 2022 Jul 19. PMID 35854001, Free PMC Article
    2. Let-7c inhibits metastatic ability of mouse hepatocarcinoma cells via targeting mannoside acetylglucosaminyltransferase 4 isoenzyme A. Guo Y, et al. Int J Biochem Cell Biol, 2014 Aug. PMID 24769441
    3. Dietary and genetic control of glucose transporter 2 glycosylation promotes insulin secretion in suppressing diabetes. Ohtsubo K, et al. Cell, 2005 Dec 29. PMID 16377570
    4. Physiological and glycomic characterization of N-acetylglucosaminyltransferase-IVa and -IVb double deficient mice. Takamatsu S, et al. Glycobiology, 2010 Jan. PMID 20015870, Free PMC Article
    5. The absence of core fucose up-regulates GnT-III and Wnt target genes: a possible mechanism for an adaptive response in terms of glycan function. Kurimoto A, et al. J Biol Chem, 2014 Apr 25. PMID 24619415, Free PMC Article

    See all (20) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. N-glycosylation by N-acetylglucosaminyltransferase IVa enhances the interaction of integrin beta1 with vimentin and promotes hepatocellular carcinoma cell motility.
      Title: N-glycosylation by N-acetylglucosaminyltransferase IVa enhances the interaction of integrin β1 with vimentin and promotes hepatocellular carcinoma cell motility.
    2. Discovery of a lectin domain that regulates enzyme activity in mouse N-acetylglucosaminyltransferase-IVa (MGAT4A).
      Title: Discovery of a lectin domain that regulates enzyme activity in mouse N-acetylglucosaminyltransferase-IVa (MGAT4A).
    3. our results suggest that let-7c may inhibit the metastatic ability of Hca-F cells, at least partially, via repressing Mgat4a activity.
      Title: Let-7c inhibits metastatic ability of mouse hepatocarcinoma cells via targeting mannoside acetylglucosaminyltransferase 4 isoenzyme A.
    4. Mutagenesis of this gene encoding GnT-4a glycosyltransferase is studied and its association with Diabetes type 2.
      Title: Dietary and genetic control of glucose transporter 2 glycosylation promotes insulin secretion in suppressing diabetes.
    Submit: New GeneRIF Correction

    Variation

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    Alleles

    Alleles of this type are documented at Mouse Genome Informatics  (MGI)
    • Targeted (2)  1 citation
    • Endonuclease-mediated (1) 

    General gene information

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    Markers

    Homology

    Gene Ontology Provided by MGI

    Function Evidence Code Pubs
    enables acetylglucosaminyltransferase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables acetylglucosaminyltransferase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables alpha-1,3-mannosylglycoprotein 4-beta-N-acetylglucosaminyltransferase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables alpha-1,3-mannosylglycoprotein 4-beta-N-acetylglucosaminyltransferase activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables glycosyltransferase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables transferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in N-glycan processing IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in protein N-linked glycosylation IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within protein N-linked glycosylation IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in protein glycosylation ISO
    Inferred from Sequence Orthology
    more info
    		  
    Component Evidence Code Pubs
    located_in Golgi apparatus IEA
    Inferred from Electronic Annotation
    more info
    		  
    is_active_in Golgi stack IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    is_active_in endoplasmic reticulum IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    is_active_in endoplasmic reticulum-Golgi intermediate compartment IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in extracellular region IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in membrane IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A
    Names
    N-acetylglucosaminyltransferase IVa
    N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase IVa
    UDP-N-acetylglucosamine: alpha-1,3-D-mannoside beta-1,4-N-acetylglucosaminyltransferase IVa
    NP_001277730.1
    NP_776295.1
    XP_006496085.1
    XP_006496087.1
    XP_006496089.1
    XP_011236828.1
    XP_017176450.1
    XP_017176453.1
    XP_017176454.1
    XP_017176455.1
    XP_017176456.1
    XP_036021327.1
    XP_036021329.1
    XP_036021332.1
    XP_036021335.1

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001290801.1 → NP_001277730.1  alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A isoform 2

      See identical proteins and their annotated locations for NP_001277730.1

      Status: VALIDATED

      Description
      Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. The encoded protein (isoform 2) is shorter than isoform 1.
      Source sequence(s)
      AC084390, AC140321, AK035332, AK076502, AK155724, AK163327
      Consensus CDS
      CCDS69882.1
      UniProtKB/Swiss-Prot
      Q812G0
      Related
      ENSMUSP00000121181.2, ENSMUST00000154819.8
      Conserved Domains (1) summary
      pfam04666
      Location:94 → 371
      Glyco_transf_54; N-Acetylglucosaminyltransferase-IV (GnT-IV) conserved region

    2. NM_173870.3 → NP_776295.1  alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A isoform 1

      See identical proteins and their annotated locations for NP_776295.1

      Status: VALIDATED

      Description
      Transcript Variant: This variant (1) represents the longer transcript and encodes the longer protein (isoform 1).
      Source sequence(s)
      AC084390, AC140321, AK035332, AK076502, AK155724, AK163327
      Consensus CDS
      CCDS14893.1
      UniProtKB/Swiss-Prot
      Q3TQT1, Q80UI0, Q812G0
      Related
      ENSMUSP00000114175.2, ENSMUST00000151952.8
      Conserved Domains (2) summary
      pfam04666
      Location:103 → 380
      Glyco_transf_54; N-Acetylglucosaminyltransferase-IV (GnT-IV) conserved region
      cd21105
      Location:287 → 302
      PGAP4-like; putative TM2 helix [structural motif]

    RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCm39 C57BL/6J

    Genomic

    1. NC_000067.7 Reference GRCm39 C57BL/6J

      Range
      37478421..37580599 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_036165442.1 → XP_036021335.1  alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A isoform X4

      UniProtKB/Swiss-Prot
      Q3TQT1, Q80UI0, Q812G0
      Conserved Domains (2) summary
      pfam04666
      Location:103 → 380
      Glyco_transf_54; N-Acetylglucosaminyltransferase-IV (GnT-IV) conserved region
      cd21105
      Location:287 → 302
      PGAP4-like; putative TM2 helix [structural motif]

    2. XM_017320964.2 → XP_017176453.1  alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A isoform X8

      Conserved Domains (1) summary
      pfam04666
      Location:1 → 135
      Glyco_transf_54; N-Acetylglucosaminyltransferase-IV (GnT-IV) conserved region

    3. XM_017320966.1 → XP_017176455.1  alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A isoform X8

      Conserved Domains (1) summary
      pfam04666
      Location:1 → 135
      Glyco_transf_54; N-Acetylglucosaminyltransferase-IV (GnT-IV) conserved region

    4. XM_006496026.2 → XP_006496089.1  alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A isoform X8

      See identical proteins and their annotated locations for XP_006496089.1

      Conserved Domains (1) summary
      pfam04666
      Location:1 → 135
      Glyco_transf_54; N-Acetylglucosaminyltransferase-IV (GnT-IV) conserved region

    5. XM_017320961.2 → XP_017176450.1  alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A isoform X7

      Conserved Domains (1) summary
      pfam04666
      Location:94 → 371
      Glyco_transf_54; N-Acetylglucosaminyltransferase-IV (GnT-IV) conserved region

    6. XM_017320965.1 → XP_017176454.1  alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A isoform X8

      Conserved Domains (1) summary
      pfam04666
      Location:1 → 135
      Glyco_transf_54; N-Acetylglucosaminyltransferase-IV (GnT-IV) conserved region

    7. XM_017320967.1 → XP_017176456.1  alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A isoform X9

      Conserved Domains (1) summary
      pfam04666
      Location:1 → 135
      Glyco_transf_54; N-Acetylglucosaminyltransferase-IV (GnT-IV) conserved region

    8. XM_006496024.3 → XP_006496087.1  alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A isoform X5

      Conserved Domains (1) summary
      pfam04666
      Location:103 → 380
      Glyco_transf_54; N-Acetylglucosaminyltransferase-IV (GnT-IV) conserved region

    9. XM_006496022.5 → XP_006496085.1  alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A isoform X4

      See identical proteins and their annotated locations for XP_006496085.1

      UniProtKB/Swiss-Prot
      Q3TQT1, Q80UI0, Q812G0
      Conserved Domains (2) summary
      pfam04666
      Location:103 → 380
      Glyco_transf_54; N-Acetylglucosaminyltransferase-IV (GnT-IV) conserved region
      cd21105
      Location:287 → 302
      PGAP4-like; putative TM2 helix [structural motif]

    10. XM_036165436.1 → XP_036021329.1  alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A isoform X2

      Conserved Domains (2) summary
      pfam04666
      Location:140 → 417
      Glyco_transf_54; N-Acetylglucosaminyltransferase-IV (GnT-IV) conserved region
      cd21105
      Location:324 → 339
      PGAP4-like; putative TM2 helix [structural motif]

    11. XM_036165439.1 → XP_036021332.1  alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A isoform X3

      Conserved Domains (2) summary
      pfam04666
      Location:131 → 408
      Glyco_transf_54; N-Acetylglucosaminyltransferase-IV (GnT-IV) conserved region
      cd21105
      Location:315 → 330
      PGAP4-like; putative TM2 helix [structural motif]

    12. XM_036165434.1 → XP_036021327.1  alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A isoform X1

      Conserved Domains (2) summary
      pfam04666
      Location:140 → 417
      Glyco_transf_54; N-Acetylglucosaminyltransferase-IV (GnT-IV) conserved region
      cd21105
      Location:324 → 339
      PGAP4-like; putative TM2 helix [structural motif]

    13. XM_011238526.3 → XP_011236828.1  alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A isoform X6

      See identical proteins and their annotated locations for XP_011236828.1

      UniProtKB/Swiss-Prot
      Q812G0
      Conserved Domains (1) summary
      pfam04666
      Location:94 → 371
      Glyco_transf_54; N-Acetylglucosaminyltransferase-IV (GnT-IV) conserved region
    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q812G0.1 GenPept UniProtKB/Swiss-Prot:Q812G0

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