Mgat2 mannoside acetylglucosaminyltransferase 2 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Mgat2provided by MGI
Official Full Name: mannoside acetylglucosaminyltransferase 2provided by MGI
Primary source: MGI:MGI:2384966
See related: Ensembl:ENSMUSG00000043998 AllianceGenome:MGI:2384966
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: GNT2; CDGS2; GNT-II; GLCNACTII
Summary: Enables alpha-1,6-mannosylglycoprotein 2-beta-N-acetylglucosaminyltransferase activity. Acts upstream of or within protein N-linked glycosylation via asparagine. Predicted to be located in Golgi apparatus. Predicted to be active in Golgi membrane. Is expressed in several structures, including alimentary system; cranium; integumental system; sensory organ; and telencephalon. Used to study congenital disorder of glycosylation type IIa. Human ortholog(s) of this gene implicated in congenital disorder of glycosylation type IIa. Orthologous to human MGAT2 (alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase). [provided by Alliance of Genome Resources, Apr 2022]
Orthologs: human all
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Genomic context

Location:: 12 C2; 12 28.73 cM

Exon count:: 1

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	12	NC_000078.7 (69230932..69233547)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	12	NC_000078.6 (69184158..69186773)

Chromosome 12 - NC_000078.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

the CD11c promoter was unexpectedly active and triggered Mgat2 deletion within multiple hematopoietic lineages, showed remarkably poor penetrance within native DC populations, and produced almost undetectable levels of green fluorescent protein signal. These findings show that the CD11c promoter is not DC-specific, and extreme care should be taken in the interpretation of data using any mouse created using the CD11c-CRE
Title: Dendritic cell-specific Mgat2 knockout mice show antigen presentation defects but reveal an unexpected CD11c expression pattern.
when the C-terminal cysteine (C334) was mutated, MGAT activity was actually higher than that of wild-type FL-MGAT2. Lastly, we determined that both transmembrane domains of MGAT2 are important for its ER localization, and that MGAT2 is present in mitochondrial-associated membranes.
Title: Membrane topology of human monoacylglycerol acyltransferase-2 and identification of regions important for its localization to the endoplasmic reticulum.
The use of 1-oleoyl-glycerol-d5 and (U13)C-TG oil followed by LC/ESI/MS/MS detection of stable-isotopic labeled DAG, TG, or glycerol provides a wide range of applications to study pathophysiological regulation of the monoacylglycerol pathway and MGAT2 activity.
Title: Metabolic tracing of monoacylglycerol acyltransferase-2 activity in vitro and in vivo.
beneficial effects of vertical sleeve gastrectomy on body weight and glucose metabolism are independent of MGAT2 activity
Title: MGAT2 deficiency and vertical sleeve gastrectomy have independent metabolic effects in the mouse.
The results suggest that MGAT2 has a pivotal role in lipid metabolism in the small intestine, and the inhibition of MGAT2 activity may be a promising strategy for the treatment of obesity-related metabolic disorders.
Title: MGAT2 deficiency ameliorates high-fat diet-induced obesity and insulin resistance by inhibiting intestinal fat absorption in mice.
analysis of developmental and nutritional regulation of monoacylglyceanalysis of developmental and nutritional regulation of monoacylglycerol lipase and monoacylglycerol acyltransferaserol lipase and monoacylglycerol acyltransferase
Title: Intestinal monoacylglycerol metabolism: developmental and nutritional regulation of monoacylglycerol lipase and monoacylglycerol acyltransferase.
investigated the expression of murine MGAT2 protein along the intestinal tract, determined its subcellular localization, and studied its regulation by diet and in db/db mouse
Title: A predominant role of acyl-CoA:monoacylglycerol acyltransferase-2 in dietary fat absorption implicated by tissue distribution, subcellular localization, and up-regulation by high fat diet.
MGAT2 may play an important role in dietary fat absorption
Title: Cloning and functional characterization of a mouse intestinal acyl-CoA:monoacylglycerol acyltransferase, MGAT2.
MGAT2 is a monoacylglycerol acyltransferase expressed in the small intestine
Title: MGAT2, a monoacylglycerol acyltransferase expressed in the small intestine.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (2) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables alpha-1,6-mannosylglycoprotein 2-beta-N-acetylglucosaminyltransferase activity	IBA Inferred from Biological aspect of Ancestor more info
enables alpha-1,6-mannosylglycoprotein 2-beta-N-acetylglucosaminyltransferase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables alpha-1,6-mannosylglycoprotein 2-beta-N-acetylglucosaminyltransferase activity	ISO Inferred from Sequence Orthology more info
enables carbohydrate binding	ISO Inferred from Sequence Orthology more info
enables glycosyltransferase activity	IEA Inferred from Electronic Annotation more info
enables manganese ion binding	ISO Inferred from Sequence Orthology more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables protein homodimerization activity	ISO Inferred from Sequence Orthology more info
enables transferase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in oligosaccharide biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in protein N-linked glycosylation	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within protein N-linked glycosylation via asparagine	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein N-linked glycosylation via asparagine	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in Golgi apparatus	ISO Inferred from Sequence Orthology more info
is_active_in Golgi membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in Golgi membrane	ISO Inferred from Sequence Orthology more info
located_in Golgi stack	IEA Inferred from Electronic Annotation more info
located_in membrane	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase

Names: N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase II; beta-1,2-N-acetylglucosaminyltransferase II; glcNAc-T II

NP_666147.1

EC 2.4.1.143

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.