Top2a topoisomerase (DNA) II alpha [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Top2aprovided by MGI
Official Full Name: topoisomerase (DNA) II alphaprovided by MGI
Primary source: MGI:MGI:98790
See related: Ensembl:ENSMUSG00000020914 AllianceGenome:MGI:98790
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Top-2
Summary: Enables DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity. Involved in female meiotic nuclear division and regulation of circadian rhythm. Acts upstream of or within several processes, including DNA conformation change; embryonic cleavage; and hematopoietic progenitor cell differentiation. Located in chromosome, centromeric region; condensed chromosome; and nucleolus. Is expressed in several structures, including alimentary system; genitourinary system; nervous system; respiratory system; and sensory organ. Orthologous to human TOP2A (DNA topoisomerase II alpha). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in CNS E11.5 (RPKM 79.9), liver E14 (RPKM 47.8) and 10 other tissues See more
Orthologs: human all
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Genomic context

Location:: 11 D; 11 62.91 cM

Exon count:: 36

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	11	NC_000077.7 (98883773..98915037, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	11	NC_000077.6 (98992947..99024199, complement)

Chromosome 11 - NC_000077.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

TOP2A modulates signaling via the AKT/mTOR pathway to promote ovarian cancer cell proliferation.
Title: TOP2A modulates signaling via the AKT/mTOR pathway to promote ovarian cancer cell proliferation.
Nucleophagy contributes to genome stability through degradation of type II topoisomerases A and B and nucleolar components.
Title: Nucleophagy contributes to genome stability through degradation of type II topoisomerases A and B and nucleolar components.
Topoisomerase IIA in adult NSCs regulates SVZ neurogenesis by transcriptional activation of Usp37.
Title: Topoisomerase IIA in adult NSCs regulates SVZ neurogenesis by transcriptional activation of Usp37.
TOP2-mediated double-strand breaks is a major driving force of thymic malignancies linked to ATM deficiency.
Title: Endogenous topoisomerase II-mediated DNA breaks drive thymic cancer predisposition linked to ATM deficiency.
RNF168 interacts with TOP2alpha to mediate its polyubiquitylation and RNF168 deficiency confers resistance to ICRF-193, a TOP2 catalytic inhibitor, and cytotoxic anti-cancer drug etoposide in cultured mouse cells.
Title: RNF168 and USP10 regulate topoisomerase IIα function via opposing effects on its ubiquitylation.
Topo IIalpha plays an important role in adipogenesis.
Title: DNA Topoisomerase IIα contributes to the early steps of adipogenesis in 3T3-L1 cells.
Polyamide functionalisation at the N1-position offers a design strategy to improve drug-like properties. Dicationic HxIP* 3 increased topo IIalpha expression and chemosensitivity to topo II-targeting agents.
Title: Modulation of topoisomerase IIα expression and chemosensitivity through targeted inhibition of NF-Y:DNA binding by a diamino p-anisyl-benzimidazole (Hx) polyamide.
TOP2 and BAF cooperate to recruit pluripotency factors, which explains some of the instructive roles of BAF complexes.
Title: TOP2 synergizes with BAF chromatin remodeling for both resolution and formation of facultative heterochromatin.
Deletion or deficiency of PTEN leads to down regulation of TOP2A, dysfunction of the decatenation checkpoint and incomplete DNA decatenation in G2 and M phases.
Title: PTEN stabilizes TOP2A and regulates the DNA decatenation.
Inhibition of DNA topoisomerase II selectively reduces the threat of tumorigenicity
Title: Inhibition of DNA topoisomerase II selectively reduces the threat of tumorigenicity following induced pluripotent stem cell-based myocardial therapy.

Submit: New GeneRIF Correction See all GeneRIFs (29)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (1) 1 citation
Endonuclease-mediated (5) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

PMC207566P1 (e-PCR)
- UniSTS:271954

Top2a (e-PCR), detects polymorphism
- UniSTS:507286

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ATP binding	ISO Inferred from Sequence Orthology more info
enables ATP-dependent activity, acting on DNA	ISO Inferred from Sequence Orthology more info
enables DNA binding	ISO Inferred from Sequence Orthology more info
enables DNA binding, bending	ISO Inferred from Sequence Orthology more info
enables DNA topoisomerase activity	ISO Inferred from Sequence Orthology more info
enables DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity	IDA Inferred from Direct Assay more info	PubMed
enables DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity	IGI Inferred from Genetic Interaction more info	PubMed
enables DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity	ISO Inferred from Sequence Orthology more info
enables chromatin binding	ISO Inferred from Sequence Orthology more info
enables isomerase activity	IEA Inferred from Electronic Annotation more info
enables magnesium ion binding	ISO Inferred from Sequence Orthology more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables nucleotide binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein heterodimerization activity	ISO Inferred from Sequence Orthology more info
enables protein homodimerization activity	ISO Inferred from Sequence Orthology more info
enables protein kinase C binding	ISO Inferred from Sequence Orthology more info
enables sequence-specific DNA binding	ISO Inferred from Sequence Orthology more info
enables ubiquitin binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in DNA damage response	ISO Inferred from Sequence Orthology more info
involved_in DNA ligation	ISO Inferred from Sequence Orthology more info
involved_in DNA metabolic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within DNA topological change	IDA Inferred from Direct Assay more info	PubMed
involved_in DNA topological change	ISO Inferred from Sequence Orthology more info
involved_in apoptotic chromosome condensation	IBA Inferred from Biological aspect of Ancestor more info
involved_in apoptotic chromosome condensation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within chromosome condensation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within chromosome segregation	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within chromosome segregation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in chromosome segregation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within embryonic cleavage	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in female meiosis chromosome separation	TAS Traceable Author Statement more info	PubMed
involved_in female meiotic nuclear division	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within hematopoietic progenitor cell differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of DNA duplex unwinding	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of apoptotic process	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of single stranded viral RNA replication via double stranded DNA intermediate	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within positive regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of circadian rhythm	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in resolution of meiotic recombination intermediates	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within rhythmic process	IEA Inferred from Electronic Annotation more info
involved_in sister chromatid segregation	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
part_of DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) complex	ISO Inferred from Sequence Orthology more info
colocalizes_with centriole	ISO Inferred from Sequence Orthology more info
located_in chromosome, centromeric region	IDA Inferred from Direct Assay more info	PubMed
located_in condensed chromosome	IDA Inferred from Direct Assay more info	PubMed
located_in condensed chromosome	IMP Inferred from Mutant Phenotype more info	PubMed
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in male germ cell nucleus	IDA Inferred from Direct Assay more info	PubMed
colocalizes_with nuclear chromosome	ISO Inferred from Sequence Orthology more info
located_in nucleolus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	TAS Traceable Author Statement more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
is_active_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	ISO Inferred from Sequence Orthology more info
part_of protein-containing complex	ISO Inferred from Sequence Orthology more info
part_of ribonucleoprotein complex	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: DNA topoisomerase 2-alpha

Names: DNA Topoisomerase II alpha; DNA topoisomerase II, alpha isozyme

NP_035753.2

EC 5.6.2.2

XP_006533216.1

EC 5.6.2.2

XP_006533217.1

EC 5.6.2.2

XP_006533218.1

EC 5.6.2.2

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_011623.2 → NP_035753.2 DNA topoisomerase 2-alpha

See identical proteins and their annotated locations for NP_035753.2

Status: VALIDATED

Source sequence(s)

AL591067

Consensus CDS

CCDS25370.1

UniProtKB/Swiss-Prot

E9PX08, Q01320

Related

ENSMUSP00000068896.8, ENSMUST00000068031.8

Conserved Domains (2) summary

PTZ00108
Location:24 → 1441

PTZ00108; DNA topoisomerase 2-like protein; Provisional

pfam08070
Location:1431 → 1517

DTHCT; DTHCT (NUC029) region

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000077.7 Reference GRCm39 C57BL/6J

Range

98883773..98915037 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006533155.4 → XP_006533218.1 DNA topoisomerase 2-alpha isoform X2

See identical proteins and their annotated locations for XP_006533218.1

Conserved Domains (2) summary

PTZ00108
Location:24 → 1440

PTZ00108; DNA topoisomerase 2-like protein; Provisional

pfam08070
Location:1430 → 1516

DTHCT; DTHCT (NUC029) region
XM_006533153.4 → XP_006533216.1 DNA topoisomerase 2-alpha isoform X1

See identical proteins and their annotated locations for XP_006533216.1

UniProtKB/Swiss-Prot

E9PX08, Q01320

Conserved Domains (2) summary

PTZ00108
Location:24 → 1441

PTZ00108; DNA topoisomerase 2-like protein; Provisional

pfam08070
Location:1431 → 1517

DTHCT; DTHCT (NUC029) region
XM_006533154.4 → XP_006533217.1 DNA topoisomerase 2-alpha isoform X2

See identical proteins and their annotated locations for XP_006533217.1

Conserved Domains (2) summary

PTZ00108
Location:24 → 1440

PTZ00108; DNA topoisomerase 2-like protein; Provisional

pfam08070
Location:1430 → 1516

DTHCT; DTHCT (NUC029) region