rrf-1 RNA-directed RNA polymerase [Caenorhabditis elegans] - Gene

Summary

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Gene symbol: rrf-1
Gene description: RNA-directed RNA polymerase
Primary source: WormBase:WBGene00004508
Locus tag: CELE_F26A3.8
See related: AllianceGenome:WB:WBGene00004508
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Caenorhabditis elegans (strain: Bristol N2)
Lineage: Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis
Summary: Enables DEAD/H-box RNA helicase binding activity and RNA-directed 5'-3' RNA polymerase activity. Involved in hermaphrodite genitalia morphogenesis and production of siRNA involved in RNA interference. Located in mutator focus and nucleus. Part of polysome. [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See rrf-1 in Genome Data Viewer

Location:: chromosome: I

Exon count:: 20

Sequence:: Chromosome: I; NC_003279.8 (7643431..7649777, complement)

Chromosome I - NC_003279.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003279.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Here we demonstrate that EGO-1 can compensate for the lack of RRF-1 when dsRNA from neurons is used to silence genes in intestinal cells.
Title: Gene silencing by double-stranded RNA from C. elegans neurons reveals functional mosaicism of RNA interference.
rrf-1 mutants display several phenotypes not previously appreciated, including broader tissue-specific RNAi-processing capabilities
Title: C. elegans rrf-1 mutations maintain RNAi efficiency in the soma in addition to the germline.
Present evidence for successive engagement of two different RdRPs (RRF-3/RRF-1) in an endogenous siRNA-based mechanism targeting specific mRNAs in C. elegans soma.
Title: Distinct phases of siRNA synthesis in an endogenous RNAi pathway in C. elegans soma.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by WormBase

Function	Evidence Code	Pubs
enables DEAD/H-box RNA helicase binding	IPI Inferred from Physical Interaction more info	PubMed
enables RNA binding	IEA Inferred from Electronic Annotation more info
enables RNA-dependent RNA polymerase activity	IBA Inferred from Biological aspect of Ancestor more info
enables RNA-dependent RNA polymerase activity	IDA Inferred from Direct Assay more info	PubMed
enables RNA-dependent RNA polymerase activity	IEA Inferred from Electronic Annotation more info
enables RNA-dependent RNA polymerase activity	ISS Inferred from Sequence or Structural Similarity more info	PubMed
enables nucleotidyltransferase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables transferase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in RNA-templated transcription	IEA Inferred from Electronic Annotation more info
involved_in regulatory ncRNA-mediated gene silencing	IEA Inferred from Electronic Annotation more info
involved_in siRNA processing	IBA Inferred from Biological aspect of Ancestor more info
involved_in siRNA processing	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in mutator focus	IDA Inferred from Direct Assay more info	PubMed
part_of nuclear RNA-directed RNA polymerase complex	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: RNA-directed RNA polymerase

NP_001250555.1

Confirmed by transcript evidence

NP_001250556.1

Confirmed by transcript evidence

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003279.8 Reference assembly

Range

7643431..7649777 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001263626.4 → NP_001250555.1 RNA-directed RNA polymerase [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_001250555.1

Status: REVIEWED

UniProtKB/TrEMBL

G5ECM1

Conserved Domains (1) summary

pfam05183
Location:479 → 1035

RdRP; RNA dependent RNA polymerase
NM_001263627.3 → NP_001250556.1 RNA-directed RNA polymerase [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_001250556.1

Status: REVIEWED

UniProtKB/TrEMBL

G3MU40

Conserved Domains (1) summary

pfam05183
Location:398 → 954

RdRP; RNA dependent RNA polymerase