Mthfd2l methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2-like [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Mthfd2lprovided by MGI
Official Full Name: methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2-likeprovided by MGI
Primary source: MGI:MGI:1915871
See related: Ensembl:ENSMUSG00000029376 AllianceGenome:MGI:1915871
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: 1110019K23Rik; C630010D07Rik
Summary: Predicted to enable methenyltetrahydrofolate cyclohydrolase activity; methylenetetrahydrofolate dehydrogenase (NAD+) activity; and methylenetetrahydrofolate dehydrogenase (NADP+) activity. Predicted to be involved in 10-formyltetrahydrofolate metabolic process and tetrahydrofolate interconversion. Predicted to act upstream of or within cellular amino acid biosynthetic process; one-carbon metabolic process; and purine nucleotide biosynthetic process. Predicted to be located in mitochondrial inner membrane and mitochondrial matrix. Predicted to be active in mitochondrion. Is expressed in several structures, including central nervous system; future brain; gonad; gut; and retina nuclear layer. Orthologous to human MTHFD2L (methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2 like). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Ubiquitous expression in CNS E14 (RPKM 10.4), whole brain E14.5 (RPKM 10.0) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 5 E1; 5 44.78 cM

Exon count:: 11

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	5	NC_000071.7 (91078704..91169227)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	5	NC_000071.6 (90930873..91021368)

Chromosome 5 - NC_000071.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

We show here that the MTHFD2L enzyme is bifunctional, possessing both CH2-THF dehydrogenase and 5,10-methenyl-THF cyclohydrolase activities.
Title: Mitochondrial MTHFD2L is a dual redox cofactor-specific methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase expressed in both adult and embryonic tissues.

Submit: New GeneRIF Correction

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (3)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables catalytic activity	IEA Inferred from Electronic Annotation more info
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables methenyltetrahydrofolate cyclohydrolase activity	IBA Inferred from Biological aspect of Ancestor more info
enables methenyltetrahydrofolate cyclohydrolase activity	ISO Inferred from Sequence Orthology more info
enables methylenetetrahydrofolate dehydrogenase (NAD+) activity	IBA Inferred from Biological aspect of Ancestor more info
enables methylenetetrahydrofolate dehydrogenase (NAD+) activity	ISO Inferred from Sequence Orthology more info
enables methylenetetrahydrofolate dehydrogenase (NADP+) activity	IBA Inferred from Biological aspect of Ancestor more info
enables methylenetetrahydrofolate dehydrogenase (NADP+) activity	ISO Inferred from Sequence Orthology more info
enables methylenetetrahydrofolate dehydrogenase [NAD(P)+] activity	IEA Inferred from Electronic Annotation more info
enables oxidoreductase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in 10-formyltetrahydrofolate metabolic process	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within amino acid biosynthetic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within histidine biosynthetic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within metabolic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within methionine biosynthetic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within one-carbon metabolic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within purine nucleotide biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in tetrahydrofolate interconversion	IBA Inferred from Biological aspect of Ancestor more info
involved_in tetrahydrofolate interconversion	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in mitochondrial inner membrane	ISO Inferred from Sequence Orthology more info
located_in mitochondrial matrix	ISO Inferred from Sequence Orthology more info
is_active_in mitochondrion	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase 2, mitochondrial

Names: MTHFD2-like; NADP-dependent methylenetetrahydrofolate dehydrogenase 2-like protein; probable bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase 2

NP_081064.1

EC 1.5.1.15

EC 1.5.1.5

EC 3.5.4.9

XP_006535239.1

EC 1.5.1.15

EC 1.5.1.5

EC 3.5.4.9

XP_006535240.1

EC 1.5.1.15

EC 1.5.1.5

EC 3.5.4.9

XP_006535241.1

EC 1.5.1.15

EC 1.5.1.5

EC 3.5.4.9

XP_030110616.1

EC 1.5.1.15

EC 1.5.1.5

EC 3.5.4.9

XP_030110617.1

EC 1.5.1.15

EC 1.5.1.5

EC 3.5.4.9

XP_030110618.1

EC 1.5.1.15

EC 1.5.1.5

EC 3.5.4.9

XP_030110619.1

EC 1.5.1.15

EC 1.5.1.5

EC 3.5.4.9

XP_030110620.1

EC 1.5.1.15

EC 1.5.1.5

EC 3.5.4.9

XP_036021300.1

EC 1.5.1.15

EC 1.5.1.5

EC 3.5.4.9

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_026788.1 → NP_081064.1 bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase 2, mitochondrial

See identical proteins and their annotated locations for NP_081064.1

Status: VALIDATED

Source sequence(s)

AC109311, AC157938, AK003824, BU610697, BY014284, CO042485

Consensus CDS

CCDS39145.1

UniProtKB/Swiss-Prot

D3YZG8

Related

ENSMUSP00000071578.5, ENSMUST00000071652.6

Conserved Domains (3) summary

cd01080
Location:155 → 334

NAD_bind_m-THF_DH_Cyclohyd; NADP binding domain of methylene-tetrahydrofolate dehydrogenase/cyclohydrolase

COG0190
Location:44 → 335

FolD; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase [Coenzyme transport and metabolism]

pfam00763
Location:44 → 160

THF_DHG_CYH; Tetrahydrofolate dehydrogenase/cyclohydrolase, catalytic domain

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000071.7 Reference GRCm39 C57BL/6J

Range

91078704..91169227

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_030254760.2 → XP_030110620.1 bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase 2, mitochondrial isoform X9

Conserved Domains (1) summary

COG0190
Location:2 → 199

FolD; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase [Coenzyme transport and metabolism]
XM_030254759.2 → XP_030110619.1 bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase 2, mitochondrial isoform X8

Conserved Domains (1) summary

COG0190
Location:72 → 234

FolD; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase [Coenzyme transport and metabolism]
XM_006535178.5 → XP_006535241.1 bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase 2, mitochondrial isoform X5

Conserved Domains (1) summary

COG0190
Location:72 → 276

FolD; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase [Coenzyme transport and metabolism]
XM_030254758.2 → XP_030110618.1 bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase 2, mitochondrial isoform X7

Conserved Domains (1) summary

COG0190
Location:2 → 244

FolD; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase [Coenzyme transport and metabolism]
XM_036165407.1 → XP_036021300.1 bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase 2, mitochondrial isoform X6

Conserved Domains (1) summary

COG0190
Location:44 → 248

FolD; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase [Coenzyme transport and metabolism]
XM_030254757.2 → XP_030110617.1 bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase 2, mitochondrial isoform X4

Conserved Domains (1) summary

COG0190
Location:2 → 286

FolD; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase [Coenzyme transport and metabolism]
XM_006535177.5 → XP_006535240.1 bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase 2, mitochondrial isoform X2

Conserved Domains (1) summary

COG0190
Location:72 → 321

FolD; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase [Coenzyme transport and metabolism]
XM_006535176.5 → XP_006535239.1 bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase 2, mitochondrial isoform X1

Conserved Domains (1) summary

COG0190
Location:72 → 363

FolD; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase [Coenzyme transport and metabolism]
XM_030254756.2 → XP_030110616.1 bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase 2, mitochondrial isoform X3

Conserved Domains (1) summary

COG0190
Location:44 → 293

FolD; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase [Coenzyme transport and metabolism]