Ppp4c protein phosphatase 4, catalytic subunit [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Ppp4cprovided by MGI
Official Full Name: protein phosphatase 4, catalytic subunitprovided by MGI
Primary source: MGI:MGI:1891763
See related: Ensembl:ENSMUSG00000030697 AllianceGenome:MGI:1891763
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Ppx; pp4; PP-X; PP4C; 1110002D08Rik
Summary: Predicted to enable lamin binding activity and protein serine/threonine phosphatase activity. Predicted to be involved in dephosphorylation and regulation of double-strand break repair via homologous recombination. Located in cytoplasm and nucleus. Part of protein phosphatase 4 complex. Is expressed in several structures, including brain; neural retina; olfactory epithelium; and thymus primordium. Orthologous to human PPP4C (protein phosphatase 4 catalytic subunit). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Ubiquitous expression in thymus adult (RPKM 94.8), CNS E11.5 (RPKM 74.7) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 7 F3; 7 69.25 cM

Exon count:: 9

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	7	NC_000073.7 (126385038..126391729, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	7	NC_000073.6 (126785866..126792557, complement)

Chromosome 7 - NC_000073.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Oligoasthenoteratospermia and sperm tail bending in PPP4C-deficient mice.
Title: Oligoasthenoteratospermia and sperm tail bending in PPP4C-deficient mice.
The PP4 is indispensable for preventing DNA replication stress that could interfere with CSR, thereby promoting antibody switching during the humoral immune response.
Title: PP4 deficiency leads to DNA replication stress that impairs immunoglobulin class switch efficiency.
LCMT-1 homozygous knock-out MEFs exhibited hyperphosphorylation of HDAC3, a reported target of the methylation-dependent PP4R1-PP4c complex. Collectively, our data suggest that LCMT-1 coordinately regulates the carboxyl methylation of PP2A-related phosphatases and, consequently, their holoenzyme assembly and function.
Title: Leucine Carboxyl Methyltransferase 1 (LCMT-1) Methylates Protein Phosphatase 4 (PP4) and Protein Phosphatase 6 (PP6) and Differentially Regulates the Stable Formation of Different PP4 Holoenzymes.
T cell-specific ablation of PP4 resulted in defective adaptive immunity, impaired T cell homeostatic expansion, and inefficient T cell proliferation.
Title: T cell proliferation and adaptive immune responses are critically regulated by protein phosphatase 4.
Data show that protein phosphatase 4 (PP4) is an important regulator in inflammatory related insulin resistance.
Title: Protein phosphatase 4 (PP4) functions as a critical regulator in tumor necrosis factor (TNF)-α-induced hepatic insulin resistance.
Thus, serine/threonine phosphatase PP4 functions as a novel feedback negative regulator of RNA virus-triggered innate immunity.
Title: Phosphatase PP4 Negatively Regulates Type I IFN Production and Antiviral Innate Immunity by Dephosphorylating and Deactivating TBK1.
Taken together, our results establish a novel role for PP4 in CSR, and reveal crucial functions for PP4 in the maintenance of genomic stability, GC formation, and B cell-mediated immune responses.
Title: PP4 is essential for germinal center formation and class switch recombination in mice.
PP4 overexpression was observed to lead to a decreased pACC1Ser79/ACC1 ratio and subsequently an increased intracellular triglyceride content in mouse primary hepatocytes.
Title: Protein phosphatase 4 promotes hepatic lipogenesis through dephosphorylating acetyl‑CoA carboxylase 1 on serine 79.
Mammalian SMEK/PP4C proteins are involved in the regulation of hepatic glucose metabolism through dephosphorylation of CRTC2.
Title: Suppressor of MEK null (SMEK)/protein phosphatase 4 catalytic subunit (PP4C) is a key regulator of hepatic gluconeogenesis.
PP4 negatively regulated LPS-induced and TRAF6-mediated NF-kappaB activation by inhibiting the ubiquitination of TRAF6.
Title: Protein phosphatase 4 negatively regulates LPS cascade by inhibiting ubiquitination of TRAF6.

Submit: New GeneRIF Correction See all GeneRIFs (12)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Gene trapped (1)
Targeted (5) 1 citation
Endonuclease-mediated (4) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

D10Mit157.2 (e-PCR), detects polymorphism
- UniSTS:126061

AU016079 (e-PCR)
- UniSTS:159693

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables lamin binding	ISO Inferred from Sequence Orthology more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables myosin phosphatase activity	IEA Inferred from Electronic Annotation more info
enables phosphatase activity	ISO Inferred from Sequence Orthology more info
enables phosphoprotein phosphatase activity	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein serine/threonine phosphatase activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein serine/threonine phosphatase activity	ISO Inferred from Sequence Orthology more info
enables protein serine/threonine phosphatase activity	NAS Non-traceable Author Statement more info	PubMed
enables protein-containing complex binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in dephosphorylation	ISO Inferred from Sequence Orthology more info
involved_in double-strand break repair via homologous recombination	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of protein phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in regulation of double-strand break repair	ISO Inferred from Sequence Orthology more info
involved_in regulation of double-strand break repair via homologous recombination	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
part_of chromatin	ISO Inferred from Sequence Orthology more info
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoskeleton	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
part_of protein phosphatase 4 complex	IDA Inferred from Direct Assay more info	PubMed
part_of protein phosphatase 4 complex	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: serine/threonine-protein phosphatase 4 catalytic subunit

Names: protein phosphatase X

NP_001347393.1

EC 3.1.3.16

NP_062648.1

EC 3.1.3.16

XP_006508112.1

EC 3.1.3.16

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001360464.1 → NP_001347393.1 serine/threonine-protein phosphatase 4 catalytic subunit

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 both encode the same protein.

Source sequence(s)

AC124505

Consensus CDS

CCDS21844.1

UniProtKB/Swiss-Prot

P97470

UniProtKB/TrEMBL

Q3UNF2

Related

ENSMUSP00000146245.2, ENSMUST00000206570.2

Conserved Domains (1) summary

cd07415
Location:6 → 290

MPP_PP2A_PP4_PP6; PP2A, PP4, and PP6 phosphoprotein phosphatases, metallophosphatase domain
NM_019674.4 → NP_062648.1 serine/threonine-protein phosphatase 4 catalytic subunit

See identical proteins and their annotated locations for NP_062648.1

Status: VALIDATED

Description

Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 both encode the same protein.

Source sequence(s)

AC124505

Consensus CDS

CCDS21844.1

UniProtKB/Swiss-Prot

P97470

UniProtKB/TrEMBL

Q3UNF2

Related

ENSMUSP00000032936.7, ENSMUST00000032936.8

Conserved Domains (1) summary

cd07415
Location:6 → 290

MPP_PP2A_PP4_PP6; PP2A, PP4, and PP6 phosphoprotein phosphatases, metallophosphatase domain

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000073.7 Reference GRCm39 C57BL/6J

Range

126385038..126391729 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006508049.2 → XP_006508112.1 serine/threonine-protein phosphatase 4 catalytic subunit isoform X1

See identical proteins and their annotated locations for XP_006508112.1

Conserved Domains (1) summary

cd07415
Location:1 → 211

MPP_PP2A_PP4_PP6; PP2A, PP4, and PP6 phosphoprotein phosphatases, metallophosphatase domain