Psmb8 proteasome (prosome, macropain) subunit, beta type 8 (large multifunctional peptidase 7) [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Psmb8provided by MGI
Official Full Name: proteasome (prosome, macropain) subunit, beta type 8 (large multifunctional peptidase 7)provided by MGI
Primary source: MGI:MGI:1346527
See related: Ensembl:ENSMUSG00000024338 AllianceGenome:MGI:1346527
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Lmp7; Lmp-7
Summary: Predicted to enable endopeptidase activity. Involved in fat cell differentiation. Acts upstream of or within antigen processing and presentation. Part of proteasome core complex and spermatoproteasome complex. Is expressed in thymus primordium. Human ortholog(s) of this gene implicated in JMP syndrome. Orthologous to human PSMB8 (proteasome 20S subunit beta 8). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Broad expression in thymus adult (RPKM 141.3), large intestine adult (RPKM 117.3) and 15 other tissues See more
Orthologs: human all
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Genomic context

Location:: 17 B1; 17 17.98 cM

Exon count:: 6

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	17	NC_000083.7 (34417169..34420428)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	17	NC_000083.6 (34198195..34201454)

Chromosome 17 - NC_000083.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Expression of immunoproteasome subunits in the brains of Toxoplasma gondii-infected mice.
Title: Expression of immunoproteasome subunits in the brains of Toxoplasma gondii-infected mice.
Macrophage pyroptosis is mediated by immunoproteasome subunit beta5i (LMP7) in abdominal aortic aneurysm.
Title: Macrophage pyroptosis is mediated by immunoproteasome subunit β5i (LMP7) in abdominal aortic aneurysm.
Ablation of Immunoproteasome beta5i Subunit Suppresses Hypertensive Retinopathy by Blocking ATRAP Degradation in Mice.
Title: Ablation of Immunoproteasome β5i Subunit Suppresses Hypertensive Retinopathy by Blocking ATRAP Degradation in Mice.
This study provides new evidence of the critical role of beta5i in deoxycorticosterone-acetate-salt-induced cardiac remodeling.
Title: Genetic ablation and pharmacological inhibition of immunosubunit β5i attenuates cardiac remodeling in deoxycorticosterone-acetate (DOCA)-salt hypertensive mice.
Knockout of beta5i(PSMB8) in cardiomyocytes and mice markedly attenuated the hypertrophic response, and this effect was aggravated by beta5i overexpression in cardiomyocytes. beta5i interacted with and promoted ATG5 degradation thereby leading to inhibition of autophagy and cardiac hypertrophy.
Title: The immunoproteasome catalytic β5i subunit regulates cardiac hypertrophy by targeting the autophagy protein ATG5 for degradation.
results provide, to our knowledge, novel evidence that ablation or pharmacological inhibition of LMP7 attenuates Ang II-induced abdominal aortic aneurysm (AAA) formation, and LMP7 might be a novel therapeutic target for treating AAA in humans
Title: Ablation and Inhibition of the Immunoproteasome Catalytic Subunit LMP7 Attenuate Experimental Abdominal Aortic Aneurysm Formation in Mice.
co-inhibition of LMP2 and LMP7 appears to be synergistic and advantageous for the treatment of autoimmune diseases.
Title: Co-inhibition of immunoproteasome subunits LMP2 and LMP7 is required to block autoimmunity.
deficiency of IP subunit beta5i/LMP7 does not alter initiation and progression of atherosclerosis in LDLR-/- mice.
Title: Immunoproteasome subunit ß5i/LMP7-deficiency in atherosclerosis.
Employing the lymphocytic choriomeningitis virus (LCMV) infection model, we showed that the immunoproteasome subunit LMP7 was absolutely required for the generation of LCMV GP118-125 -specific T cells although the class I mediated presentation of GP118-125 was not dependent on LMP7.
Title: The immunoproteasome subunit LMP7 is required in the murine thymus for filling up a hole in the T cell repertoire.
Proteostatic responses included a significant increase in the levels of Lmp7, a component of the immunoproteasome. Increased Lmp7 levels and activity were also quantified in postmortem human brains with PD and dementia with Lewy bodies.
Title: Induction of the Immunoproteasome Subunit Lmp7 Links Proteostasis and Immunity in α-Synuclein Aggregation Disorders.

Submit: New GeneRIF Correction See all GeneRIFs (34)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (3) 1 citation
Endonuclease-mediated (1) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

RH139868 (e-PCR)
- UniSTS:221952

D2Mit171 (e-PCR), detects polymorphism
- UniSTS:129039

M55637 (e-PCR)
- UniSTS:181008

M55637 (e-PCR)
- UniSTS:124955

L11145 (e-PCR)
- UniSTS:160649

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables endopeptidase activity	IBA Inferred from Biological aspect of Ancestor more info
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables peptidase activity	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables threonine-type endopeptidase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within antigen processing and presentation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within cell differentiation	IEA Inferred from Electronic Annotation more info
involved_in fat cell differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in fat cell differentiation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within immune system process	IEA Inferred from Electronic Annotation more info
involved_in proteasome-mediated ubiquitin-dependent protein catabolic process	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within proteolysis	IEA Inferred from Electronic Annotation more info
involved_in proteolysis involved in protein catabolic process	IEA Inferred from Electronic Annotation more info
involved_in regulation of endopeptidase activity	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	TAS Traceable Author Statement more info
located_in nucleoplasm	TAS Traceable Author Statement more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
part_of proteasome complex	ISO Inferred from Sequence Orthology more info
part_of proteasome core complex	IDA Inferred from Direct Assay more info	PubMed
part_of proteasome core complex, beta-subunit complex	IBA Inferred from Biological aspect of Ancestor more info
part_of spermatoproteasome complex	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: proteasome subunit beta type-8

Names: MC13 (LMP7); large multifunctional protease 7; low molecular mass protein 7; macropain subunit C13; multicatalytic endopeptidase complex subunit C13; proteasome component C13; proteasome subunit MC13; proteasome subunit beta-5i; proteosome (prosome, macropain) subunit, beta type 8 (large multifunctional peptidase 7); proteosome beta 8 subunit

NP_034854.2

EC 3.4.25.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_010724.2 → NP_034854.2 proteasome subunit beta type-8

See identical proteins and their annotated locations for NP_034854.2

Status: VALIDATED

Source sequence(s)

AK031801, AK150674, CJ235673

Consensus CDS

CCDS50073.1

UniProtKB/Swiss-Prot

P28063, Q3UK42, Q64300, Q792S8, Q7TMX9, Q91VH7

UniProtKB/TrEMBL

A0A068BIT8

Related

ENSMUSP00000025196.9, ENSMUST00000025196.9

Conserved Domains (2) summary

PTZ00488
Location:40 → 271

PTZ00488; Proteasome subunit beta type-5; Provisional

cd03761
Location:73 → 260

proteasome_beta_type_5; proteasome beta type-5 subunit. The 20S proteasome, multisubunit proteolytic complex, is the central enzyme of nonlysosomal protein degradation in both the cytosol and nucleus. It is composed of 28 subunits arranged as four homoheptameric rings that ...