RHOJ ras homolog family member J [Homo sapiens (human)] - Gene

Summary

Official Symbol: RHOJprovided by HGNC
Official Full Name: ras homolog family member Jprovided by HGNC
Primary source: HGNC:HGNC:688
See related: Ensembl:ENSG00000126785 MIM:607653; AllianceGenome:HGNC:688
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: TCL; ARHJ; TC10B; RASL7B
Summary: This gene encodes one of the many small GTP-binding proteins in the Rho family shown to be associated with focal adhesions in endothelial cells (PMID: 21148427, 22103495). The encoded protein is activated by vascular endothelial growth factor and may regulate angiogenesis. [provided by RefSeq, Dec 2011]
Expression: Ubiquitous expression in gall bladder (RPKM 9.9), lung (RPKM 9.2) and 23 other tissues See more
Orthologs: mouse all
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Genomic context

Location:: 14q23.2

Exon count:: 6

Annotation release	Status	Assembly	Chr	Location
RS_2023_10	current	GRCh38.p14 (GCF_000001405.40)	14	NC_000014.9 (63204443..63293508)
RS_2023_10	current	T2T-CHM13v2.0 (GCF_009914755.1)	14	NC_060938.1 (57411795..57500853)
105.20220307	previous assembly	GRCh37.p13 (GCF_000001405.25)	14	NC_000014.8 (63671161..63760226)

Chromosome 14 - NC_000014.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: HPA RNA-seq normal tissues
Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
BioProject: PRJEB4337
Publication: PMID 24309898
Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Towards Understanding the Development of Breast Cancer: The Role of RhoJ in the Obesity Microenvironment.
Title: Towards Understanding the Development of Breast Cancer: The Role of RhoJ in the Obesity Microenvironment.
RHOJ Induces Epithelial-to-Mesenchymal Transition by IL-6/STAT3 to Promote Invasion and Metastasis in Gastric Cancer.
Title: RHOJ Induces Epithelial-to-Mesenchymal Transition by IL-6/STAT3 to Promote Invasion and Metastasis in Gastric Cancer.
Repression of RhoJ expression promotes TGF-beta-mediated EMT in human non-small-cell lung cancer A549cells.
Title: Repression of RhoJ expression promotes TGF-β-mediated EMT in human non-small-cell lung cancer A549cells.
Rhoj Is a Novel Target for Progression and Invasion of Glioblastoma by Impairing Cytoskeleton Dynamics.
Title: Rhoj Is a Novel Target for Progression and Invasion of Glioblastoma by Impairing Cytoskeleton Dynamics.
A Protective Role for RHOJ in NonSmall Cell Lung Cancer Based on Integrated Bioinformatics Analysis.
Title: A Protective Role for RHOJ in NonSmall Cell Lung Cancer Based on Integrated Bioinformatics Analysis.
RhoJ Regulates alpha5beta1 Integrin Trafficking to Control Fibronectin Remodeling during Angiogenesis.
Title: RhoJ Regulates α5β1 Integrin Trafficking to Control Fibronectin Remodeling during Angiogenesis.
RhoJ integrates attractive and repulsive cues in directional migration of endothelial cells.
Title: RhoJ integrates attractive and repulsive cues in directional migration of endothelial cells.
Short-term treatment of nascent melanoma tumors with PAK inhibitors that block RhoJ signaling halts the growth of BRAF mutant melanoma tumors in vivo and induces apoptosis in melanoma cells in vitro via a BAD-dependent mechanism. As up to 50% of BRAF mutant human melanomas express high levels of RhoJ, these studies nominate the RhoJ-BAD signaling network as a therapeutic vulnerability for fledgling BRAF mutant human tumor
Title: The RhoJ-BAD signaling network: An Achilles' heel for BRAF mutant melanomas.
These results identify amino acids within the N terminus and a loop region distal to the nucleotide binding pocket of TCL capable of allosterically regulating nucleotide exchange and thus influence membrane association of the protein.
Title: TCL/RhoJ Plasma Membrane Localization and Nucleotide Exchange Is Coordinately Regulated by Amino Acids within the N Terminus and a Distal Loop Region.
FMNL3 interacts with Cdc42 and RhoJ, two Rho family GTPases known to be required for lumen formation. FMNL3 and RhoJ are concentrated at the early apical surface, or AMIS, and regulate the formation of radiating actin cables from this site.
Title: The Formin FMNL3 Controls Early Apical Specification in Endothelial Cells by Regulating the Polarized Trafficking of Podocalyxin.

Submit: New GeneRIF Correction See all GeneRIFs (19)

Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

D11S2921 (e-PCR)
- UniSTS:152074

RH65935 (e-PCR)
- UniSTS:52290

RH69284 (e-PCR)
- UniSTS:32589

STS-AA011182 (e-PCR)
- UniSTS:83300

D10S16 (e-PCR)
- UniSTS:155756

RH77820 (e-PCR)
- UniSTS:55672

G35452 (e-PCR)
- UniSTS:46971

D14S1151 (e-PCR)
- UniSTS:20862

ARHJ_4040 (e-PCR)
- UniSTS:463547

Gene Ontology Provided by GOA

Function	Evidence Code	Pubs
enables GTP binding	IBA Inferred from Biological aspect of Ancestor more info
enables GTP binding	IDA Inferred from Direct Assay more info	PubMed
enables GTPase activity	IBA Inferred from Biological aspect of Ancestor more info
enables GTPase activity	IDA Inferred from Direct Assay more info	PubMed
enables GTPase activity	NAS Non-traceable Author Statement more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase binding	IBA Inferred from Biological aspect of Ancestor more info

Process	Evidence Code	Pubs
involved_in Cdc42 protein signal transduction	IBA Inferred from Biological aspect of Ancestor more info
involved_in actin cytoskeleton organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in actin filament organization	IBA Inferred from Biological aspect of Ancestor more info
involved_in angiogenesis	IEA Inferred from Electronic Annotation more info
involved_in endocytosis	IBA Inferred from Biological aspect of Ancestor more info
involved_in establishment or maintenance of cell polarity	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of cell migration involved in sprouting angiogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of cell shape	IEA Inferred from Electronic Annotation more info
involved_in regulation of endothelial cell migration	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of sprouting angiogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in retina vasculature morphogenesis in camera-type eye	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in extracellular exosome	HDA more info	PubMed
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	TAS Traceable Author Statement more info

General protein information

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Preferred Names: rho-related GTP-binding protein RhoJ

Names: RAS-like, family 7, member B; TC10-like Rho GTPase; ras homolog gene family, member J; ras-like protein family member 7B; tc10-like GTP-binding protein

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_020663.5 → NP_065714.1 rho-related GTP-binding protein RhoJ

See identical proteins and their annotated locations for NP_065714.1

Status: VALIDATED

Source sequence(s)

AF087862, AK027351, AL049871, BM555132

Consensus CDS

CCDS9757.1

UniProtKB/Swiss-Prot

Q96KC1, Q9H4E5

UniProtKB/TrEMBL

Q7Z513

Related

ENSP00000316729.3, ENST00000316754.8

Conserved Domains (1) summary

cl38936
Location:22 → 195

P-loop_NTPase; P-loop containing Nucleoside Triphosphate Hydrolases

RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2023_10

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p14 Primary Assembly

Genomic

NC_000014.9 Reference GRCh38.p14 Primary Assembly

Range

63204443..63293508

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_011536993.4 → XP_011535295.1 rho-related GTP-binding protein RhoJ isoform X2

Conserved Domains (2) summary

smart00174
Location:24 → 142

RHO; Rho (Ras homology) subfamily of Ras-like small GTPases

cl21455
Location:22 → 140

P-loop_NTPase; P-loop containing Nucleoside Triphosphate Hydrolases
XM_047431613.1 → XP_047287569.1 rho-related GTP-binding protein RhoJ isoform X1

Related

ENSP00000451643.1, ENST00000555125.1