Ddo D-aspartate oxidase [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Ddoprovided by MGI
Official Full Name: D-aspartate oxidaseprovided by MGI
Primary source: MGI:MGI:1925528
See related: Ensembl:ENSMUSG00000063428 AllianceGenome:MGI:1925528
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: DASPO; 5330420D20Rik; 5730402C02Rik
Summary: Enables D-aspartate oxidase activity and FAD binding activity. Involved in D-amino acid catabolic process. Acts upstream of or within several processes, including aspartate metabolic process; grooming behavior; and insemination. Predicted to be located in cytosol. Predicted to be active in cytoplasm. Is expressed in several structures, including alimentary system; chordamesoderm; nervous system; respiratory system; and sensory organ. Orthologous to human DDO (D-aspartate oxidase). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in kidney adult (RPKM 4.3), heart adult (RPKM 4.1) and 12 other tissues See more
Orthologs: human all
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Genomic context

Location:: 10 B1; 10 22.0 cM

Exon count:: 10

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	10	NC_000076.7 (40505933..40557847)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	10	NC_000076.6 (40630011..40681847)

Chromosome 10 - NC_000076.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

D-aspartate oxidase gene duplication induces social recognition memory deficit in mice and intellectual disabilities in humans.
Title: D-aspartate oxidase gene duplication induces social recognition memory deficit in mice and intellectual disabilities in humans.
Prenatal expression of D-aspartate oxidase causes early cerebral D-aspartate depletion and influences brain morphology and cognitive functions at adulthood.
Title: Prenatal expression of D-aspartate oxidase causes early cerebral D-aspartate depletion and influences brain morphology and cognitive functions at adulthood.
In this framework, an epiallele is defined as a specific combination of methylated CpG within Ddo locus and can represent the epigenetic haplotype revealing a cell-to-cell methylation heterogeneity.
Title: Tracking the evolution of epialleles during neural differentiation and brain development: D-Aspartate oxidase as a model gene.
This study demonstraed that age-related reduction of free d-Asp levels in the mouse brain in dod knockout mice.
Title: Age-Related Changes in D-Aspartate Oxidase Promoter Methylation Control Extracellular D-Aspartate Levels and Prevent Precocious Cell Death during Brain Aging.
Ddo(-/-) mice display a significant reduction in schizophrenia related symptoms induced by phencyclidine. Increased D-aspartate in Ddo(-/-) animals affect the development of cortico-hippocampal connectivity networks potentially involved in schizophrenia.
Title: A role for D-aspartate oxidase in schizophrenia and in schizophrenia-related symptoms induced by phencyclidine in mice.
Arg-216 and Arg-237 play crucial roles in the substrate specificity of DDO.
Title: Role of the active site residues arginine-216 and arginine-237 in the substrate specificity of mammalian D-aspartate oxidase.
reports that the behavioral phenotype of the Dco-/- mouse is characterized by deficits in prepulse inhibition of the acoustic startle reflex and in motor coordination on the RotoRod
Title: Behavioural alterations in male mice lacking the gene for D-aspartate oxidase.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (2) 1 citation
Endonuclease-mediated (3)

General gene information

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Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables D-amino-acid oxidase activity	IEA Inferred from Electronic Annotation more info
enables D-aspartate oxidase activity	IBA Inferred from Biological aspect of Ancestor more info
enables D-aspartate oxidase activity	IDA Inferred from Direct Assay more info	PubMed
enables D-aspartate oxidase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables D-aspartate oxidase activity	ISO Inferred from Sequence Orthology more info
enables FAD binding	IDA Inferred from Direct Assay more info	PubMed
enables FAD binding	ISO Inferred from Sequence Orthology more info
enables oxidoreductase activity	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in D-amino acid catabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in D-amino acid catabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in D-amino acid catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in D-amino acid catabolic process	ISO Inferred from Sequence Orthology more info
involved_in D-amino acid metabolic process	IEA Inferred from Electronic Annotation more info
involved_in aspartate catabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in aspartate catabolic process	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within aspartate metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within grooming behavior	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within hormone metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within insemination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in nervous system process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in nervous system process	ISO Inferred from Sequence Orthology more info
involved_in regulation of cell communication	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
is_active_in peroxisome	ISO Inferred from Sequence Orthology more info
located_in peroxisome	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: D-aspartate oxidase

Names: DASOX

NP_001303645.1

EC 1.4.3.1

NP_001303646.1

EC 1.4.3.1

NP_001303647.1

EC 1.4.3.1

NP_001303648.1

EC 1.4.3.1

NP_081718.2

EC 1.4.3.1

XP_036011895.1

EC 1.4.3.1

XP_036011896.1

EC 1.4.3.1

XP_036011897.1

EC 1.4.3.1

XP_036011898.1

EC 1.4.3.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001316716.2 → NP_001303645.1 D-aspartate oxidase isoform 2

Status: VALIDATED

Description

Transcript Variant: This variant (2) uses an alternate in-frame splice junction in the 5' end and has an alternate last exon compared to variant 1. The resulting isoform (2) contains an alternate internal segment and has a shorter and distinct C-terminus compared to isoform 1.

Source sequence(s)

AC139760

Consensus CDS

CCDS87992.1

UniProtKB/TrEMBL

A0A1L1SS55, Q9CYP3

Related

ENSMUSP00000149767.2, ENSMUST00000214102.2

Conserved Domains (1) summary

pfam01266
Location:6 → 166

DAO; FAD dependent oxidoreductase
NM_001316717.2 → NP_001303646.1 D-aspartate oxidase isoform 3 precursor

Status: VALIDATED

Description

Transcript Variant: This variant (3) has an alternate last exon compared to variant 1. The resulting isoform (3) has a shorter and distinct C-terminus compared to isoform 1.

Source sequence(s)

AC139760

Consensus CDS

CCDS87995.1

UniProtKB/TrEMBL

Q9CYP3

Related

ENSMUSP00000150979.2, ENSMUST00000216830.2

Conserved Domains (1) summary

pfam01266
Location:6 → 157

DAO; FAD dependent oxidoreductase
NM_001316718.2 → NP_001303647.1 D-aspartate oxidase isoform 4 precursor

Status: VALIDATED

Description

Transcript Variant: This variant (4) has an alternate last exon compared to variant 1. The resulting isoform (4) has a shorter and distinct C-terminus compared to isoform 1.

Source sequence(s)

AC139760

Consensus CDS

CCDS87994.1

UniProtKB/TrEMBL

Q9CYP3, Q9D2B4

Related

ENSMUSP00000151066.2, ENSMUST00000213503.2

Conserved Domains (1) summary

pfam01266
Location:6 → 128

DAO; FAD dependent oxidoreductase
NM_001316719.2 → NP_001303648.1 D-aspartate oxidase isoform 5 precursor

Status: VALIDATED

Description

Transcript Variant: This variant (5) has an alternate last exon compared to variant 1. The resulting isoform (5) has a shorter and distinct C-terminus compared to isoform 1.

Source sequence(s)

AC139760

Consensus CDS

CCDS87993.1

UniProtKB/TrEMBL

A0A1L1SS65, Q9CYP3

Related

ENSMUSP00000149768.2, ENSMUST00000213442.2

Conserved Domains (1) summary

pfam01266
Location:6 → 128

DAO; FAD dependent oxidoreductase
NM_027442.6 → NP_081718.2 D-aspartate oxidase isoform 1 precursor

See identical proteins and their annotated locations for NP_081718.2

Status: VALIDATED

Description

Transcript Variant: This variant (1) encodes the longest isoform (1).

Source sequence(s)

AC139760

Consensus CDS

CCDS23798.1

UniProtKB/Swiss-Prot

Q0E9Q7, Q2UVA9, Q3TT69, Q8R2R2, Q922Z0

Related

ENSMUSP00000019977.8, ENSMUST00000019977.8

Conserved Domains (1) summary

pfam01266
Location:6 → 324

DAO; FAD dependent oxidoreductase

RNA

NR_133568.2 RNA Sequence

Status: VALIDATED

Description

Transcript Variant: This variant (6) lacks an alternate internal exon and contains two 3' exons in place of the last exon compared to variant 1. This variant is represented as non-coding because the use of the 5'-most expected translational start codon renders the transcript a candidate for nonsense-mediated mRNA decay (NMD).

Source sequence(s)

AC139760

Related

ENSMUST00000213856.2

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000076.7 Reference GRCm39 C57BL/6J

Range

40505933..40557847

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_036156002.1 → XP_036011895.1 D-aspartate oxidase isoform X1

Conserved Domains (1) summary

pfam01266
Location:27 → 206

DAO; FAD dependent oxidoreductase
XM_036156003.1 → XP_036011896.1 D-aspartate oxidase isoform X2

UniProtKB/TrEMBL

Q9CYP3

Conserved Domains (1) summary

pfam01266
Location:6 → 129

DAO; FAD dependent oxidoreductase
XM_036156004.1 → XP_036011897.1 D-aspartate oxidase isoform X3

UniProtKB/TrEMBL

Q9CYP3

Conserved Domains (1) summary

pfam01266
Location:6 → 129

DAO; FAD dependent oxidoreductase
XM_036156005.1 → XP_036011898.1 D-aspartate oxidase isoform X4

UniProtKB/TrEMBL

Q9CYP3

Conserved Domains (1) summary

pfam01266
Location:6 → 129

DAO; FAD dependent oxidoreductase