Cyth2 cytohesin 2 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Cyth2provided by MGI
Official Full Name: cytohesin 2provided by MGI
Primary source: MGI:MGI:1334255
See related: Ensembl:ENSMUSG00000003269 AllianceGenome:MGI:1334255
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: ARNO; CLM2; Pscd2
Summary: Enables guanyl-nucleotide exchange factor activity and inositol 1,4,5 trisphosphate binding activity. Involved in dendrite morphogenesis and endosome organization. Located in several cellular components, including bicellular tight junction; cell body; and endosome. Part of BLOC-1 complex. Is active in cytoplasm; dendritic shaft; and dendritic spine. Is expressed in brain and spinal cord. Orthologous to human CYTH2 (cytohesin 2). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Ubiquitous expression in CNS E11.5 (RPKM 32.8), CNS E14 (RPKM 32.4) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 7 B3; 7 29.52 cM

Exon count:: 14

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	7	NC_000073.7 (45456058..45463857, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	7	NC_000073.6 (45806637..45814322, complement)

Chromosome 7 - NC_000073.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The adaptor SH2B1 and the phosphatase PTP4A1 regulate the phosphorylation of cytohesin-2 in myelinating Schwann cells in mice.
Title: The adaptor SH2B1 and the phosphatase PTP4A1 regulate the phosphorylation of cytohesin-2 in myelinating Schwann cells in mice.
Deletion of Arno Reduces Eosinophilic Inflammation and Interleukin-5 Expression in a Murine Model of Rhinitis.
Title: Deletion of Arno Reduces Eosinophilic Inflammation and Interleukin-5 Expression in a Murine Model of Rhinitis.
Cytohesin-2/ARNO: A Novel Bridge Between Cell Migration and Immunoregulation in Synovial Fibroblasts.
Title: Cytohesin-2/ARNO: A Novel Bridge Between Cell Migration and Immunoregulation in Synovial Fibroblasts.
Cytohesin-2 mediates group I metabotropic glutamate receptor-dependent mechanical allodynia through the activation of ADP ribosylation factor 6 in the spinal cord.
Title: Cytohesin-2 mediates group I metabotropic glutamate receptor-dependent mechanical allodynia through the activation of ADP ribosylation factor 6 in the spinal cord.
The synaptic scaffolding protein CNKSR2 interacts with CYTH2 to mediate hippocampal granule cell development.
Title: The synaptic scaffolding protein CNKSR2 interacts with CYTH2 to mediate hippocampal granule cell development.
Structural organization and dynamics of Grp1 and cytohesin-2 in solution and on membranes has been described.
Title: Structural Organization and Dynamics of Homodimeric Cytohesin Family Arf GTPase Exchange Factors in Solution and on Membranes.
results show that ARNO interaction with the RLIP76 N-terminus regulates cell spreading and motility via PI3K and Arf6, independent of RLIP76 control of Rac
Title: The RLIP76 N-terminus binds ARNO to regulate PI 3-kinase, Arf6 and Rac signaling, cell spreading and migration.
The RLIP76/R-Ras/ARNO complex localizes to recycling endosomes.
Title: RLIP76 regulates Arf6-dependent cell spreading and migration by linking ARNO with activated R-Ras at recycling endosomes.
signaling through cytohesin-2 is required for myelination by Schwann cells, and cytohesin-2 is added to the list of molecules known to underlie PNS myelination
Title: Arf6 guanine-nucleotide exchange factor cytohesin-2 regulates myelination in nerves.
results together suggest that cytohesin 2 activates ARF6 in a PI3K dependent manner and then the active ARF6 causes phosphorylation of ERK1/2 during preadipocyte migration.
Title: Cytohesin 2/ARF6 regulates preadipocyte migration through the activation of ERK1/2.

Submit: New GeneRIF Correction See all GeneRIFs (17)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (3) 1 citation
Endonuclease-mediated (1)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables guanyl-nucleotide exchange factor activity	IDA Inferred from Direct Assay more info	PubMed
enables inositol 1,4,5 trisphosphate binding	IDA Inferred from Direct Assay more info	PubMed
enables lipid binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in negative regulation of dendrite development	ISO Inferred from Sequence Orthology more info
involved_in regulation of ARF protein signal transduction	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within regulation of cell adhesion	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in anchoring junction	IEA Inferred from Electronic Annotation more info
located_in bicellular tight junction	IDA Inferred from Direct Assay more info	PubMed
located_in cell projection	IEA Inferred from Electronic Annotation more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
is_active_in extrinsic component of postsynaptic specialization membrane	ISO Inferred from Sequence Orthology more info
is_active_in glutamatergic synapse	IDA Inferred from Direct Assay more info	PubMed
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
is_active_in postsynapse	IDA Inferred from Direct Assay more info	PubMed
located_in ruffle	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: cytohesin-2

Names: ARF nucleotide-binding site opener; PH, SEC7 and coiled-coil domain-containing protein 2; SEC7 homolog B; mSec7-2; pleckstrin homology, Sec7 and coiled-coil domains 2

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001112701.2 → NP_001106171.1 cytohesin-2 isoform 2

See identical proteins and their annotated locations for NP_001106171.1

Status: VALIDATED

Description

Transcript Variant: This variant (2) lacks an in-frame short exon of 3 nt compared to variant 1. The resulting isoform (2) is shorter by 1 aa compared to isoform 1.

Source sequence(s)

AC149053

Consensus CDS

CCDS52249.1

UniProtKB/TrEMBL

A0A8Z1RZY0, Q99KH2

Related

ENSMUSP00000103357.2, ENSMUST00000107729.10

Conserved Domains (2) summary

cd01252
Location:258 → 377

PH_GRP1-like; General Receptor for Phosphoinositides-1-like Pleckstrin homology (PH) domain

pfam01369
Location:61 → 243

Sec7; Sec7 domain
NM_011181.4 → NP_035311.1 cytohesin-2 isoform 1

See identical proteins and their annotated locations for NP_035311.1

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

Source sequence(s)

AC149053, AK045451, AK143829, BE983477, BY470655

UniProtKB/Swiss-Prot

E9QJX3, P63034, Q3UP39

UniProtKB/TrEMBL

A0A8Z1RZY0, Q5HZZ0

Conserved Domains (2) summary

cd01252
Location:258 → 378

PH_GRP1-like; General Receptor for Phosphoinositides-1-like Pleckstrin homology (PH) domain

pfam01369
Location:61 → 243

Sec7; Sec7 domain

RNA

NR_175963.1 RNA Sequence

Status: VALIDATED

Source sequence(s)

AC149053

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000073.7 Reference GRCm39 C57BL/6J

Range

45456058..45463857 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_036152796.1 → XP_036008689.1 cytohesin-2 isoform X1

UniProtKB/TrEMBL

A0A1B0GRB2

Related

ENSMUSP00000147457.2, ENSMUST00000209245.2

Conserved Domains (2) summary

pfam01369
Location:61 → 243

Sec7; Sec7 domain

cl17171
Location:258 → 270

PH-like; Pleckstrin homology-like domain