Trim28 tripartite motif-containing 28 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Trim28provided by MGI
Official Full Name: tripartite motif-containing 28provided by MGI
Primary source: MGI:MGI:109274
See related: Ensembl:ENSMUSG00000005566 AllianceGenome:MGI:109274
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: KAP-1; Tif1b; KRIP-1; MommeD9; Tif1beta
Summary: Enables DNA binding activity; protein kinase activity; and transcription coactivator activity. Involved in epithelial to mesenchymal transition and positive regulation of DNA methylation-dependent heterochromatin assembly. Acts upstream of or within several processes, including DNA methylation involved in embryo development; negative regulation of single stranded viral RNA replication via double stranded DNA intermediate; and regulation of nucleobase-containing compound metabolic process. Located in euchromatin; heterochromatin; and nucleoplasm. Part of RNA polymerase II transcription regulator complex. Is expressed in several structures, including branchial arch; central nervous system; early conceptus; genitourinary system; and limb primordium. Orthologous to human TRIM28 (tripartite motif containing 28). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Ubiquitous expression in ovary adult (RPKM 143.7), CNS E11.5 (RPKM 133.2) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 7 A1; 7 7.73 cM

Exon count:: 17

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	7	NC_000073.7 (12758079..12764959)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	7	NC_000073.6 (13024152..13031032)

Chromosome 7 - NC_000073.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Heat shock protein gp96 drives natural killer cell maturation and anti-tumor immunity by counteracting Trim28 to stabilize Eomes.
Title: Heat shock protein gp96 drives natural killer cell maturation and anti-tumor immunity by counteracting Trim28 to stabilize Eomes.
Co-option of endogenous retroviruses through genetic escape from TRIM28 repression.
Title: Co-option of endogenous retroviruses through genetic escape from TRIM28 repression.
Loss of Trim28 in muscle alters mitochondrial signalling but not systemic metabolism.
Title: Loss of Trim28 in muscle alters mitochondrial signalling but not systemic metabolism.
TRIM28 promotes the escape of gastric cancer cells from immune surveillance by increasing PD-L1 abundance.
Title: TRIM28 promotes the escape of gastric cancer cells from immune surveillance by increasing PD-L1 abundance.
Mitochondrial Glycerol-3-Phosphate Dehydrogenase Restricts HBV Replication via the TRIM28-Mediated Degradation of HBx.
Title: Mitochondrial Glycerol-3-Phosphate Dehydrogenase Restricts HBV Replication via the TRIM28-Mediated Degradation of HBx.
TRIM28 represses renal cell carcinoma cell proliferation by inhibiting TFE3/KDM6A-regulated autophagy.
Title: TRIM28 represses renal cell carcinoma cell proliferation by inhibiting TFE3/KDM6A-regulated autophagy.
TRIM28 promotes luminal cell plasticity in a mouse model of prostate cancer.
Title: TRIM28 promotes luminal cell plasticity in a mouse model of prostate cancer.
Trim28 citrullination maintains mouse embryonic stem cell pluripotency via regulating Nanog and Klf4 transcription.
Title: Trim28 citrullination maintains mouse embryonic stem cell pluripotency via regulating Nanog and Klf4 transcription.
TRIM28 secures skeletal stem cell fate during skeletogenesis by silencing neural gene expression and repressing GREM1/AKT/mTOR signaling axis.
Title: TRIM28 secures skeletal stem cell fate during skeletogenesis by silencing neural gene expression and repressing GREM1/AKT/mTOR signaling axis.
TRIM28-dependent SUMOylation protects the adult ovary from activation of the testicular pathway.
Title: TRIM28-dependent SUMOylation protects the adult ovary from activation of the testicular pathway.

Submit: New GeneRIF Correction See all GeneRIFs (79)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (5) 1 citation
Chemically induced (ENU) (3) 1 citation
Endonuclease-mediated (7) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

RH126533 (e-PCR)
- UniSTS:209673

RH137168 (e-PCR)
- UniSTS:219256

Trim28 (e-PCR), detects polymorphism
- UniSTS:527586

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables DNA binding	ISO Inferred from Sequence Orthology more info
enables Krueppel-associated box domain binding	ISO Inferred from Sequence Orthology more info
enables SUMO transferase activity	ISO Inferred from Sequence Orthology more info
enables chromatin binding	IBA Inferred from Biological aspect of Ancestor more info
enables chromatin binding	ISO Inferred from Sequence Orthology more info
enables chromo shadow domain binding	ISO Inferred from Sequence Orthology more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables promoter-specific chromatin binding	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables transcription coactivator activity	IDA Inferred from Direct Assay more info	PubMed
enables transcription corepressor activity	ISO Inferred from Sequence Orthology more info
enables transferase activity	IEA Inferred from Electronic Annotation more info
enables ubiquitin protein ligase activity	IBA Inferred from Biological aspect of Ancestor more info
enables ubiquitin protein ligase binding	ISO Inferred from Sequence Orthology more info
enables ubiquitin-protein transferase activity	ISO Inferred from Sequence Orthology more info
enables zinc ion binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in DNA repair	ISO Inferred from Sequence Orthology more info
involved_in chromatin organization	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within convergent extension involved in axis elongation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within embryo implantation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within embryonic placenta morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within epigenetic programming of gene expression	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in epithelial to mesenchymal transition	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within genomic imprinting	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within in utero embryonic development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in innate immune response	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within innate immune response	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within innate immune response	IGI Inferred from Genetic Interaction more info	PubMed
involved_in innate immune response	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within negative regulation of DNA demethylation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of DNA-templated transcription	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within negative regulation of DNA-templated transcription	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of DNA-templated transcription	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of DNA-templated transcription	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within negative regulation of single stranded viral RNA replication via double stranded DNA intermediate	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within negative regulation of single stranded viral RNA replication via double stranded DNA intermediate	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within negative regulation of single stranded viral RNA replication via double stranded DNA intermediate	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of single stranded viral RNA replication via double stranded DNA intermediate	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within negative regulation of transcription by RNA polymerase II	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within negative regulation of transcription by RNA polymerase II	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within positive regulation of DNA binding	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within positive regulation of DNA binding	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of DNA methylation-dependent heterochromatin formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of DNA methylation-dependent heterochromatin formation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of DNA repair	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within positive regulation of DNA-templated transcription	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of DNA-templated transcription	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of protein import into nucleus	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within protein autophosphorylation	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within protein phosphorylation	IDA Inferred from Direct Assay more info	PubMed
involved_in protein sumoylation	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein sumoylation	ISO Inferred from Sequence Orthology more info
involved_in suppression of viral release by host	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
part_of RNA polymerase II transcription regulator complex	IBA Inferred from Biological aspect of Ancestor more info
part_of RNA polymerase II transcription regulator complex	IDA Inferred from Direct Assay more info	PubMed
part_of chromatin	IDA Inferred from Direct Assay more info	PubMed
part_of euchromatin	IDA Inferred from Direct Assay more info	PubMed
part_of heterochromatin	IDA Inferred from Direct Assay more info	PubMed
NOT located_in nucleolus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	ISO Inferred from Sequence Orthology more info
part_of protein-containing complex	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: transcription intermediary factor 1-beta

Names: E3 SUMO-protein ligase TRIM28; KRAB-A-interacting protein; RING-type E3 ubiquitin transferase TIF1-beta; TIF1-beta; transcriptional intermediary factor 1, beta; tripartite motif protein 28; tripartite motif-containing protein 28

NP_035718.2

EC 2.3.2.27

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_011588.3 → NP_035718.2 transcription intermediary factor 1-beta

See identical proteins and their annotated locations for NP_035718.2

Status: VALIDATED

Source sequence(s)

BC089337

Consensus CDS

CCDS20823.1

UniProtKB/Swiss-Prot

P70391, Q62318, Q8C283, Q99PN4

UniProtKB/TrEMBL

Q5EBP9

Related

ENSMUSP00000005705.8, ENSMUST00000005705.8

Conserved Domains (6) summary

smart00502
Location:253 → 379

BBC; B-Box C-terminal domain

smart00336
Location:149 → 185

BBOX; B-Box-type zinc finger

cd00021
Location:208 → 246

BBOX; B-Box-type zinc finger; zinc binding domain (CHC3H2); often present in combination with other motifs, like RING zinc finger, NHL motif, coiled-coil or RFP domain in functionally unrelated proteins, most likely mediating protein-protein interaction.

cd15623
Location:627 → 669

PHD_TIF1beta; PHD finger found in transcription intermediary factor 1-beta (TIF1-beta)

pfam14634
Location:65 → 123

zf-RING_5; zinc-RING finger domain

cl02556
Location:696 → 802

Bromodomain; Bromodomain. Bromodomains are found in many chromatin-associated proteins and in nuclear histone acetyltransferases. They interact specifically with acetylated lysine.