Dgkb diacylglycerol kinase, beta [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Dgkbprovided by MGI
Official Full Name: diacylglycerol kinase, betaprovided by MGI
Primary source: MGI:MGI:2442474
See related: Ensembl:ENSMUSG00000036095 AllianceGenome:MGI:2442474
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: DGK; 90kda; DAGK2; DGK-beta; mKIAA0718; 6430574F24; C630029D13Rik
Summary: Enables diacylglycerol kinase activity. Involved in modulation of chemical synaptic transmission and regulation of postsynapse organization. Acts upstream of or within response to bacterium. Located in Schaffer collateral - CA1 synapse; glutamatergic synapse; and plasma membrane. Is expressed in anterior abdominal wall musculature; nervous system; retina; and urinary system. Orthologous to human DGKB (diacylglycerol kinase beta). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in frontal lobe adult (RPKM 12.9), cortex adult (RPKM 7.5) and 2 other tissues See more
Orthologs: human all
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Genomic context

Location:: 12 A3; 12 17.11 cM

Exon count:: 27

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	12	NC_000078.7 (37930169..38684238)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	12	NC_000078.6 (37880169..38634239)

Chromosome 12 - NC_000078.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Memantine, an NMDA receptor antagonist, improves working memory deficits in Diacylglycerol kinase (DGK) beta knockout mice. These findings may support the hypothesis that DGKbeta has a pivotal role in cognitive function.
Title: Memantine, an NMDA receptor antagonist, improves working memory deficits in DGKβ knockout mice.
These findings suggest that DGKbeta regulates the spine formation at distal dendrites in medium spiny neurons
Title: Involvement of diacylglycerol kinase β in the spine formation at distal dendrites of striatal medium spiny neurons.
This study demonistrated that Dgkb distribuate in mice cerebellum
Title: Differential distribution of phospholipase C beta isoforms and diaglycerol kinase-beta in rodents cerebella corroborates the division of unipolar brush cells into two major subtypes.
forced expression of diacylglycerol kinase beta abrogated DAG accumulation at the phagosome, leading to impaired respiratory burst.
Title: Diacylglycerol kinases terminate diacylglycerol signaling during the respiratory burst leading to heterogeneous phagosomal NADPH oxidase activation.
that DGKbeta KO mice showed attention-deficit and hyperactive phenotype, similar to ADHD.
Title: Diacylglycerol kinase β knockout mice exhibit attention-deficit behavior and an abnormal response on methylphenidate-induced hyperactivity.
Results demonstrate that membrane-localized DGKbeta regulates spine formation by regulation of lipids, contributing to the maintenance of neural networks in synaptic transmission of cognitive processes including memory.
Title: Essential role of neuron-enriched diacylglycerol kinase (DGK), DGKbeta in neurite spine formation, contributing to cognitive function.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Transposon induced (1) 1 citation
Gene trapped (1)
Endonuclease-mediated (2)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

26.MMHAP97FRA11.seq (e-PCR)
- UniSTS:123765

DGKB_V4 (e-PCR)
- UniSTS:277159

DGKB_583 (e-PCR)
- UniSTS:277159

Dgkb (e-PCR), detects polymorphism
- UniSTS:546732

px-27c2 (e-PCR)
- UniSTS:257498

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP-dependent diacylglycerol kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables ATP-dependent diacylglycerol kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables ATP-dependent diacylglycerol kinase activity	ISO Inferred from Sequence Orthology more info
enables calcium ion binding	IEA Inferred from Electronic Annotation more info
enables kinase activity	IEA Inferred from Electronic Annotation more info
enables lipid binding	ISO Inferred from Sequence Orthology more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables nucleotide binding	IEA Inferred from Electronic Annotation more info
enables transferase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in diacylglycerol metabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in diacylglycerol metabolic process	ISO Inferred from Sequence Orthology more info
involved_in glycerolipid metabolic process	ISO Inferred from Sequence Orthology more info
involved_in intracellular signal transduction	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within lipid metabolic process	IEA Inferred from Electronic Annotation more info
involved_in lipid phosphorylation	IBA Inferred from Biological aspect of Ancestor more info
involved_in lipid phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in modulation of chemical synaptic transmission	IDA Inferred from Direct Assay more info	PubMed
involved_in modulation of chemical synaptic transmission	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in phosphatidic acid biosynthetic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in phosphatidic acid biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in phosphatidic acid metabolic process	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within phosphorylation	IEA Inferred from Electronic Annotation more info
involved_in protein kinase C-activating G protein-coupled receptor signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in regulation of postsynapse organization	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of postsynapse organization	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within response to bacterium	IEP Inferred from Expression Pattern more info	PubMed
involved_in signal transduction	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
is_active_in Schaffer collateral - CA1 synapse	IDA Inferred from Direct Assay more info	PubMed
is_active_in Schaffer collateral - CA1 synapse	IMP Inferred from Mutant Phenotype more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
is_active_in extrinsic component of postsynaptic density membrane	ISO Inferred from Sequence Orthology more info
is_active_in glutamatergic synapse	IDA Inferred from Direct Assay more info	PubMed
is_active_in glutamatergic synapse	IMP Inferred from Mutant Phenotype more info	PubMed
is_active_in glutamatergic synapse	ISO Inferred from Sequence Orthology more info
located_in membrane	IEA Inferred from Electronic Annotation more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
located_in postsynaptic membrane	IEA Inferred from Electronic Annotation more info
located_in synapse	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: diacylglycerol kinase beta

Names: DAG kinase beta; diglyceride kinase beta

NP_001348615.1

EC 2.7.1.107

NP_001348617.1

EC 2.7.1.107

NP_848796.2

EC 2.7.1.107

XP_006515130.1

EC 2.7.1.107

XP_006515131.1

EC 2.7.1.107

XP_006515133.1

EC 2.7.1.107

XP_006515134.1

EC 2.7.1.107

XP_017170508.1

EC 2.7.1.107

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001361686.1 → NP_001348615.1 diacylglycerol kinase beta isoform 1

Status: VALIDATED

Source sequence(s)

AC116471, AC141479, AC157494, AC157821, AC166996, CT009742, CT025577

UniProtKB/Swiss-Prot

Q6NS52, Q80TT5

Conserved Domains (6) summary

smart00045
Location:580 → 760

DAGKa; Diacylglycerol kinase accessory domain (presumed)

smart00046
Location:438 → 559

DAGKc; Diacylglycerol kinase catalytic domain (presumed)

cd00029
Location:244 → 293

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

cd00051
Location:152 → 221

EFh; EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to ...

pfam00130
Location:309 → 358

C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)

pfam14513
Location:6 → 148

DAG_kinase_N; Diacylglycerol kinase N-terminus
NM_001361688.1 → NP_001348617.1 diacylglycerol kinase beta isoform 2

Status: VALIDATED

Source sequence(s)

AK139408, AK161933, AW493391, BC070461, BG088459

Consensus CDS

CCDS25889.1

Related

ENSMUSP00000152378.2, ENSMUST00000220990.2

Conserved Domains (6) summary

smart00045
Location:573 → 753

DAGKa; Diacylglycerol kinase accessory domain (presumed)

smart00046
Location:431 → 552

DAGKc; Diacylglycerol kinase catalytic domain (presumed)

cd00029
Location:237 → 286

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

cd00051
Location:145 → 214

EFh; EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to ...

pfam00130
Location:302 → 353

C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)

pfam14513
Location:7 → 141

DAG_kinase_N; Diacylglycerol kinase N-terminus
NM_178681.5 → NP_848796.2 diacylglycerol kinase beta isoform 2

See identical proteins and their annotated locations for NP_848796.2

Status: VALIDATED

Source sequence(s)

AK139408, AW493391, BG088459, CJ128305

Consensus CDS

CCDS25889.1

UniProtKB/Swiss-Prot

Q6NS52

Related

ENSMUSP00000037900.8, ENSMUST00000040500.9

Conserved Domains (6) summary

smart00045
Location:573 → 753

DAGKa; Diacylglycerol kinase accessory domain (presumed)

smart00046
Location:431 → 552

DAGKc; Diacylglycerol kinase catalytic domain (presumed)

cd00029
Location:237 → 286

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

cd00051
Location:145 → 214

EFh; EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to ...

pfam00130
Location:302 → 353

C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)

pfam14513
Location:7 → 141

DAG_kinase_N; Diacylglycerol kinase N-terminus

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000078.7 Reference GRCm39 C57BL/6J

Range

37930169..38684238

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006515068.4 → XP_006515131.1 diacylglycerol kinase beta isoform X1

See identical proteins and their annotated locations for XP_006515131.1

Conserved Domains (6) summary

smart00045
Location:581 → 761

DAGKa; Diacylglycerol kinase accessory domain (presumed)

smart00046
Location:439 → 560

DAGKc; Diacylglycerol kinase catalytic domain (presumed)

cd00029
Location:244 → 293

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

cd00051
Location:152 → 221

EFh; EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to ...

pfam00130
Location:309 → 358

C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)

pfam14513
Location:6 → 148

DAG_kinase_N; Diacylglycerol kinase N-terminus
XM_017315019.3 → XP_017170508.1 diacylglycerol kinase beta isoform X2

UniProtKB/Swiss-Prot

Q6NS52, Q80TT5

Conserved Domains (6) summary

smart00045
Location:580 → 760

DAGKa; Diacylglycerol kinase accessory domain (presumed)

smart00046
Location:438 → 559

DAGKc; Diacylglycerol kinase catalytic domain (presumed)

cd00029
Location:244 → 293

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

cd00051
Location:152 → 221

EFh; EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to ...

pfam00130
Location:309 → 358

C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)

pfam14513
Location:6 → 148

DAG_kinase_N; Diacylglycerol kinase N-terminus
XM_006515071.3 → XP_006515134.1 diacylglycerol kinase beta isoform X3

See identical proteins and their annotated locations for XP_006515134.1

Conserved Domains (6) summary

smart00045
Location:574 → 754

DAGKa; Diacylglycerol kinase accessory domain (presumed)

smart00046
Location:432 → 553

DAGKc; Diacylglycerol kinase catalytic domain (presumed)

cd00029
Location:237 → 286

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

cd00051
Location:145 → 214

EFh; EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to ...

pfam00130
Location:302 → 351

C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)

pfam14513
Location:6 → 141

DAG_kinase_N; Diacylglycerol kinase N-terminus
XM_006515070.4 → XP_006515133.1 diacylglycerol kinase beta isoform X3

See identical proteins and their annotated locations for XP_006515133.1

Conserved Domains (6) summary

smart00045
Location:574 → 754

DAGKa; Diacylglycerol kinase accessory domain (presumed)

smart00046
Location:432 → 553

DAGKc; Diacylglycerol kinase catalytic domain (presumed)

cd00029
Location:237 → 286

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

cd00051
Location:145 → 214

EFh; EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to ...

pfam00130
Location:302 → 351

C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)

pfam14513
Location:6 → 141

DAG_kinase_N; Diacylglycerol kinase N-terminus
XM_006515067.3 → XP_006515130.1 diacylglycerol kinase beta isoform X1

See identical proteins and their annotated locations for XP_006515130.1

Conserved Domains (6) summary

smart00045
Location:581 → 761

DAGKa; Diacylglycerol kinase accessory domain (presumed)

smart00046
Location:439 → 560

DAGKc; Diacylglycerol kinase catalytic domain (presumed)

cd00029
Location:244 → 293

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

cd00051
Location:152 → 221

EFh; EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to ...

pfam00130
Location:309 → 358

C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)

pfam14513
Location:6 → 148

DAG_kinase_N; Diacylglycerol kinase N-terminus