Trim25 tripartite motif-containing 25 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Trim25provided by MGI
Official Full Name: tripartite motif-containing 25provided by MGI
Primary source: MGI:MGI:102749
See related: Ensembl:ENSMUSG00000000275 AllianceGenome:MGI:102749
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: EFP; Zfp147
Summary: Predicted to enable RIG-I binding activity; transcription coactivator activity; and ubiquitin protein ligase activity. Involved in defense response to other organism; negative regulation of viral entry into host cell; and regulation of protein localization. Acts upstream of or within cellular response to leukemia inhibitory factor. Located in cytoplasm and nucleus. Is expressed in several structures, including alimentary system; central nervous system; genitourinary system; respiratory system; and sensory organ. Orthologous to human TRIM25 (tripartite motif containing 25). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Ubiquitous expression in placenta adult (RPKM 10.1), adrenal adult (RPKM 9.4) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 11 C; 11 54.34 cM

Exon count:: 9

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	11	NC_000077.7 (88890194..88911119)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	11	NC_000077.6 (88999403..89020294)

Chromosome 11 - NC_000077.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

TRIM25 inhibition attenuates inflammation, senescence, and oxidative stress in microvascular endothelial cells induced by hyperglycemia.
Title: TRIM25 inhibition attenuates inflammation, senescence, and oxidative stress in microvascular endothelial cells induced by hyperglycemia.
Long noncoding RNA AVAN promotes antiviral innate immunity by interacting with TRIM25 and enhancing the transcription of FOXO3a.
Title: Long noncoding RNA AVAN promotes antiviral innate immunity by interacting with TRIM25 and enhancing the transcription of FOXO3a.
ATAD2 drives colorectal cancer progression by regulating TRIM25 expression via a positive feedback loop with E2F transcriptional factors.
Title: ATAD2 drives colorectal cancer progression by regulating TRIM25 expression via a positive feedback loop with E2F transcriptional factors.
OTUD5 cooperates with TRIM25 in transcriptional regulation and tumor progression via deubiquitination activity.
Title: OTUD5 cooperates with TRIM25 in transcriptional regulation and tumor progression via deubiquitination activity.
RNA Helicase LGP2 Negatively Regulates RIG-I Signaling by Preventing TRIM25-Mediated Caspase Activation and Recruitment Domain Ubiquitination.
Title: RNA Helicase LGP2 Negatively Regulates RIG-I Signaling by Preventing TRIM25-Mediated Caspase Activation and Recruitment Domain Ubiquitination.
Riplet, and not TRIM25, is required endogenously for the ubiquitination of RIG-I.
Title: RIPLET, and not TRIM25, is required for endogenous RIG-I-dependent antiviral responses.
The nucleotide-binding domain of NLRP12 interacts with the ubiquitin ligase TRIM25 to prevent TRIM25-mediated, Lys63-linked ubiquitination and activation of RIG-I.
Title: NLRP12 Regulates Anti-viral RIG-I Activation via Interaction with TRIM25.
TRIM25 as a key determinant of breast cancer metastasis.
Title: An Integrated Systems Biology Approach Identifies TRIM25 as a Key Determinant of Breast Cancer Metastasis.
Studies indicated that mice with disrupted Trim25 were viable and fertile, uterine response to oestrogen was greatly attenuated and the uteri were underdeveloped.
Title: The role of Trim25 in development, disease and RNA metabolism.
Two key residues (Asp(488) and Trp(621)) in the TRIM25 B30.2 domain are critical for binding to the RIG-I CARDs.
Title: Crystal structure of the TRIM25 B30.2 (PRYSPRY) domain: a key component of antiviral signalling.

Submit: New GeneRIF Correction See all GeneRIFs (11)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Gene trapped (1)
Targeted (3) 1 citation
Endonuclease-mediated (6)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Trim25 (e-PCR), detects polymorphism
- UniSTS:547383

RH125330 (e-PCR)
- UniSTS:162152

AA960166 (e-PCR)
- UniSTS:160141

Trim25 (e-PCR), detects polymorphism
- UniSTS:144821

UniSTS:257235 (e-PCR)
- UniSTS:257235

Trim25 (e-PCR), detects polymorphism
- UniSTS:257235

UniSTS:257236 (e-PCR)
- UniSTS:257236

Trim25 (e-PCR), detects polymorphism
- UniSTS:257236

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables RIG-I binding	ISO Inferred from Sequence Orthology more info
enables ligase activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables transcription coactivator activity	ISO Inferred from Sequence Orthology more info
enables transferase activity	IEA Inferred from Electronic Annotation more info
enables ubiquitin protein ligase activity	ISO Inferred from Sequence Orthology more info
enables ubiquitin-protein transferase activity	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in ERAD pathway	ISO Inferred from Sequence Orthology more info
involved_in antiviral innate immune response	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within cellular response to leukemia inhibitory factor	IEP Inferred from Expression Pattern more info	PubMed
involved_in cytoplasmic pattern recognition receptor signaling pathway	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within defense response to virus	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within immune system process	IEA Inferred from Electronic Annotation more info
involved_in innate immune response	IDA Inferred from Direct Assay more info	PubMed
involved_in innate immune response	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of viral entry into host cell	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of DNA-binding transcription factor activity	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of NF-kappaB transcription factor activity	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of canonical NF-kappaB signal transduction	ISO Inferred from Sequence Orthology more info
involved_in protein K48-linked ubiquitination	ISO Inferred from Sequence Orthology more info
involved_in protein monoubiquitination	ISO Inferred from Sequence Orthology more info
involved_in regulation of protein localization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of viral entry into host cell	ISO Inferred from Sequence Orthology more info
involved_in response to estrogen	ISO Inferred from Sequence Orthology more info
involved_in response to oxidative stress	ISO Inferred from Sequence Orthology more info
involved_in suppression of viral release by host	IDA Inferred from Direct Assay more info	PubMed
involved_in ubiquitin-dependent protein catabolic process	ISO Inferred from Sequence Orthology more info
involved_in viral release from host cell	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytoplasmic stress granule	ISO Inferred from Sequence Orthology more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in nuclear body	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: E3 ubiquitin/ISG15 ligase TRIM25

Names: RING-type E3 ubiquitin transferase TRIM25; estrogen-responsive finger protein; tripartite motif protein 25; tripartite motif-containing protein 25; ubiquitin/ISG15-conjugating enzyme TRIM25; zinc finger protein 147

NP_033572.2

EC 2.3.2.27

EC 6.3.2.n3

XP_017169965.1

EC 2.3.2.27

EC 6.3.2.n3

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_009546.2 → NP_033572.2 E3 ubiquitin/ISG15 ligase TRIM25

See identical proteins and their annotated locations for NP_033572.2

Status: VALIDATED

Source sequence(s)

AK169562, AL646096, BC028430

Consensus CDS

CCDS36278.1

UniProtKB/Swiss-Prot

Q5SU70, Q61510

UniProtKB/TrEMBL

Q3U3X7

Related

ENSMUSP00000103528.4, ENSMUST00000107896.10

Conserved Domains (3) summary

COG1842
Location:229 → 360

PspA; Phage shock protein A [Transcription, Signal transduction mechanisms]

cd13736
Location:464 → 632

SPRY_PRY_TRIM25; PRY/SPRY domain in tripartite motif-containing domain 25 (TRIM25)

cl17238
Location:13 → 53

RING; RING-finger (Really Interesting New Gene) domain, a specialized type of Zn-finger of 40 to 60 residues that binds two atoms of zinc; defined by the 'cross-brace' motif C-X2-C-X(9-39)-C-X(1-3)- H-X(2-3)-(N/C/H)-X2-C-X(4-48)C-X2-C; probably involved in ...

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000077.7 Reference GRCm39 C57BL/6J

Range

88890194..88911119

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_017314476.3 → XP_017169965.1 E3 ubiquitin/ISG15 ligase TRIM25 isoform X1

UniProtKB/TrEMBL

Q3TU94, Q5SU71

Conserved Domains (5) summary

COG1842
Location:229 → 360

PspA; Phage shock protein A [Transcription, Signal transduction mechanisms]

cd13736
Location:431 → 599

SPRY_PRY_TRIM25; PRY/SPRY domain in tripartite motif-containing domain 25 (TRIM25)

cd19776
Location:154 → 190

Bbox2_TRIM25_C-IV; B-box-type 2 zinc finger found in tripartite motif-containing protein 25 (TRIM25) and similar proteins

cd19842
Location:105 → 151

Bbox1_TRIM25-like_C-IV; B-box-type 1 zinc finger found in tripartite motif-containing proteins, TRIM25, TRIM47 and similar proteins

cl17238
Location:11 → 54

RING_Ubox; The superfamily of RING finger (Really Interesting New Gene) domain and U-box domain